LOCUS       sequence-244355-       10592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding glutathione 
            S-transferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_GST
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3916..4569
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4527..4549
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     terminator      4617..4804
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4672..4696)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4879..6235
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4879..5222
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5223..6032
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5290..5309)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6038..6235
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6330..6347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6330..6346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6339..6361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9241..10335
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa
     3961 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc
     4021 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt
     4081 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata
     4141 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt
     4201 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt
     4261 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat
     4321 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg
     4381 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa
     4441 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc
     4501 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat
     4561 cctccaaaat cggatctggt tccgcgttga ggcgcgccag atctacttct aaataagcga
     4621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     4681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     4741 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta
     4801 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg
     4861 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     4921 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     4981 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     5041 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     5101 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     5161 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     5221 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg
     5281 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     5341 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     5401 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     5461 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     5521 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     5581 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     5641 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     5701 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     5761 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     5821 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     5881 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     5941 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     6001 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt
     6061 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     6121 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     6181 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     6241 gattcgatac taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta
     6301 tatacgaagt tatgcggccg cgcgaattca ctggccgtcg ttttacaacg tcgtgactgg
     6361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6481 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6541 ccatcgtccg gatcaatgaa gtagatgagt ttactacctg tatatacttt tttttttgtc
     6601 aaagtcactt ttatggcatt tccatgttga aggattagcc acagcactaa attttcagcg
     6661 tatgattgct tttaaatatt taattttcat cgttttttaa ttaatattca aaacgattta
     6721 atgccttcta catctgaagt caagctcata gactcgggtt gggtagctaa aactacatct
     6781 aacacgaagg ggtatagcct tcttaaagta tactcatgta taaacgtttt gggttcatat
     6841 ttttgggcca cgtggtttat aaaaattctt aactacacca ctcggtatcc cgcacccgtc
     6901 tacgtttcta cgatttcgag ctaatattga gtaaagtgat tagcaaaaaa aataaaagta
     6961 ctcgttgtcg gagatcaaga atttttctat tatctcatct aaaccacttt ctaaaagcga
     7021 aaaacaaaat cgtaatatgc agcttgaatg ggcttccata gtttgaaaga aaaaccctag
     7081 cagtactggc aagggagaca ttccttttac ctggctaatc gccatcttcc agcaggcgca
     7141 ccattgcccc tgtttcacta tccaggttac ggatatagtt catgacaata tttacattgg
     7201 tccagccacc agcttgcatg atctccggta ttgaaactcc agcgcgggcc atatctcgcg
     7261 cggctccgac acgggcactg tgtccagacc aggccaggta tctctgacca gagtcatcct
     7321 tagcgccgta aatcaatcga tgagttgctt caaaaatccc ttccagggcg cgagttgata
     7381 gctggctggt ggcagatggc gcggcaacac cattttttct gacccggcaa aacaggtagt
     7441 tattcggatc atcagctaca ccagagacgg aaatccatcg ctcgaccagt ttagttaccc
     7501 ccaggctaag tgccttctct acacctgcgg tgctaaccag cgttttcgtt ctgccaatat
     7561 ggattaacat tctcccaccg tcagtacgtg agatatcttt aaccctgatc ctggcaattt
     7621 cggctatacg taacagggtg ttataagcaa tccccagaaa tgccagatta cgtatatcct
     7681 ggcagcgatc gctattttcc atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg
     7741 ctagagcctg ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa
     7801 ccagtgaaac agcattgctg tcacttggtc gtggcagccc ggaccgacga tgaagcatgt
     7861 ttagctggcc caaatgttgc tggatagttt ttactgccag accgcgcgcc tgaagatata
     7921 gaagataatc gcgaacatct tcaggttctg cgggaaacca tttccggtta ttcaacttgc
     7981 accatgccgc ccacgaccgg caaacggaca gaagcatttt ccaggtatgc tcagaaaacg
     8041 cctggcgatc cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga
     8101 ccggtaactt ttagattaac atttgtttaa atatgaaatt aggcaatgta acatactgca
     8161 ggcaaatttt ggtgtacggt aagtaaattg gacatatgat ttaacaaagc gactataagt
     8221 cagaaagtga gaatgagatt gaaataatta attcaactta ttcaattgat gatatgccag
     8281 gattcctctt cctttatctt caatgtttcg attaacaggt ccattatccg gtttttagtt
     8341 tcgctgcaca ttgccgaatg acaatcggat tcctttaagc tttgcttttg ccctgttttc
     8401 tctccccagt aatcgttggg tgactgaacc atccagtagc cgagagttac gtttttcctg
     8461 atttaataaa tatccatatc tatttgtctc gtggtgtttt acagcaacta accgaaatta
     8521 ctattttgca tcatccaacc ataaagagac aacaataatt ctagaaaaca tataacaagt
     8581 gtttttttct atttacgtct catacgcaaa gtccacttta ccatgttttg gaaacttgta
     8641 cccaaccatt catttttcga ttttgtaatg aggctctttt cttctaacaa tcattgaggt
     8701 cattatcatg tgtcctcacc tattgtttaa attgaggctt gcgctattcc caaaattgtg
     8761 ggtattattt tcatatatca gtatctgcaa tttcggttca actccggaag ctcggtaccc
     8821 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
     8881 ttttttttct ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg
     8941 atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg
     9001 aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga
     9061 gaagcgttca tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg
     9121 acctattgtt ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct
     9181 tttacatttc agcaatatat atatatatat atatatatat ttcaaggata taccattgta
     9241 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca
     9301 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat
     9361 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat
     9421 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct
     9481 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt
     9541 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct
     9601 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgttagaga attagtggga
     9661 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa
     9721 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa
     9781 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga
     9841 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat ttcctacatt gaaagttcaa
     9901 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt
     9961 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca
    10021 ggctccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca
    10081 tttggtttgt acgaaccatg ccatggttcc gctccagatt tgccaaagaa taaggtcaac
    10141 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa
    10201 gaaggtaaag ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt
    10261 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag
    10321 aaaatccttg cttaaaaaga ttctcttttt ttgtgatatt tgtacataaa ctttataaat
    10381 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga
    10441 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa
    10501 tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta
    10561 acattaatat tgacaaggag gagggcatca ca
//
