LOCUS       sequence-244356-        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Flag-Daxx in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Tang J, Agrawal T, Cheng Q, Qu L, Brewer MD, Chen J, Yang X
  TITLE     Phosphorylation of Daxx by ATM contributes to DNA damage-induced p53
            activation.
  JOURNAL   PLoS One. 2013;8(2):e55813. doi: 10.1371/journal.pone.0055813. Epub 
            2013 Feb 6.
  PUBMED    23405218
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e55813. doi"; pages: " 
            10.1371/journal.pone.0055813. Epub 2013 Feb 6"
COMMENT     Sequence Label: pBabe-Flag-Daxx-Puro
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1386..1395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1395..1418
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3656..3676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3661..3990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3841..3976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4000..4599
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4000..4019)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4496..4516
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4820..5289
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5419..5436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5590..6178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5670..5689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6338..7129,7130..7198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6961..6980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7199..7303)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcgccgc catggactac aaggacgacg atgacaaggg atccgccacc gctaacagca
     1441 tcatcgtgct ggatgatgat gacgaagatg aagcagctgc tcagccaggg ccctcccacc
     1501 cactccccaa tgcggcctca cctggggcag aagcccctag ctcctctgag cctcatgggg
     1561 ccagaggaag cagtagttcg ggcggcaaga aatgctacaa gctggagaat gagaagctgt
     1621 tcgaagagtt ccttgaactt tgtaagatgc agacagcaga ccaccctgag gtggtcccat
     1681 tcctctataa ccggcagcaa cgtgcccact ctctgttttt ggcctcggcg gagttctgca
     1741 acatcctctc tagggtcctg tctcgggccc ggagccggcc agccaagctc tatgtctaca
     1801 tcaatgagct ctgcactgtt ctcaaggccc actcagccaa aaagaagctg aacttggccc
     1861 ctgccgccac cacctccaat gagccctctg ggaataaccc tcccacacac ctctccttgg
     1921 accccacaaa tgctgaaaac actgcctctc agtctccaag gacccgtggt tcccggcggc
     1981 agatccagcg tttggagcag ctgctggcgc tctatgtggc agagatccgg cggctgcagg
     2041 aaaaggagtt ggatctctca gaattggatg acccagactc cgcatacctg caggaggcac
     2101 ggttgaagcg taagctgatc cgcctctttg ggcgactatg tgagctgaaa gactgctctt
     2161 cactgaccgg ccgtgtcata gagcagcgca tcccctaccg tggcacccgc tacccagagg
     2221 ttaacaggcg cattgagcgg ctcatcaaca agccagggcc tgataccttc cctgactatg
     2281 gggatgtgct tcgggctgta gagaaggcag ctgcccgaca cagccttggc ctcccccgac
     2341 agcagctcca gctcatggct caggatgcct tccgagatgt gggcatcagg ttacaggagc
     2401 gacgtcacct cgatctcatc tacaactttg gctgccacct cacagatgac tataggccag
     2461 gcgttgaccc tgcactatca gatcctgtgt tggcccggcg ccttcgggaa aaccggagtt
     2521 tggccatgag tcggctggat gaggtcatct ccaaatacgc aatgttgcaa gacaaaagtg
     2581 aggagggcga gagaaaaaag agaagagctc ggctccaagg cacctcttcc cactctgcag
     2641 acacccccga agcctccttg gattctggtg agggccctag tggaatggca tcccaggggt
     2701 gcccttctgc ctccagagct gagacagatg acgaagacga tgaggagagt gatgaggaag
     2761 aggaggagga ggaggaagaa gaagaggagg aggccacaga ttctgaagag gaggaggatc
     2821 tggaacagat gcaggagggt caggaggatg atgaagagga ggacgaagag gaagaagcag
     2881 cagcaggtaa agatggagac aagagcccca tgtcctcact acagatctcc aatgaaaaga
     2941 acctggaacc tggcaaacag atcagcagat cttcagggga gcagcaaaac aaaggacgca
     3001 tagtgtcacc atcgttactg tcagaagaac ccctggcccc ctccagcata gatgctgaaa
     3061 gcaatggaga acagcctgag gagctgaccc tggaggaaga aagccctgtg tctcagctct
     3121 ttgagctaga gattgaagct ttgcccctgg ataccccttc ctctgtggag acggacattt
     3181 cctcttccag gaagcaatca gaggagccct tcaccactgt cttagagaat ggagcaggca
     3241 tggtctcttc tacttccttc aatggaggcg tctctcctca caactgggga gattctggtc
     3301 ccccctgcaa aaaatctcgg aaggagaaga agcaaacagg atcagggcca ttaggaaaca
     3361 gctatgtgga aaggcaaagg tcagtgcatg agaagaatgg gaaaaagata tgtaccctgc
     3421 ccagcccacc ttcccccttg gcttccttgg ccccagttgc tgattcctcc acgagggtgg
     3481 actctcccag ccatggcctg gtgaccagct ccctctgcat cccttctcca gcccggctgt
     3541 cccaaacccc ccattcacag cctcctcggc ctggtacttg caagacaagt gtggccacac
     3601 aatgcgatcc agaagagatc atcgtgcttt cagactctga ttaggtcgac cctgtggaat
     3661 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3721 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3781 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3841 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3901 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3961 ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta caagcccacg
     4021 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     4081 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     4141 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4201 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4261 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4321 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4381 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4441 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4501 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4561 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     4621 cgcccgaccg aaaggagcgc acgaccccat gcatcgataa aataaaagat tttatttagt
     4681 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     4741 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
     4801 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     4861 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     4921 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     4981 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     5041 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     5101 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     5161 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     5221 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     5281 ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     5341 taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat
     6301 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6361 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6421 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6481 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6541 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6601 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6661 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6721 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6781 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6841 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6901 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6961 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7021 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7081 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7141 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7201 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7261 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7321 tgccacctgc agc
//
