LOCUS       sequence-244363-        8819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentivector with tet-inducible DUXB (codon sequences 
            altered).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Chew GL, Campbell AE, De Neef E, Sutliff NA, Shadle SC, Tapscott SJ,
            Bradley RK
  TITLE     DUX4 Suppresses MHC Class I to Promote Cancer Immune Evasion and 
            Resistance to Checkpoint Blockade.
  JOURNAL   Dev Cell. 2019 Sep 9;50(5):658-671.e7. doi: 
            10.1016/j.devcel.2019.06.011. Epub 2019 Jul 18.
  PUBMED    31327741
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.06.011"; journalName: "Dev Cell"; date: 
            "2019-09-9- 9"; volume: "50"; issue: "5"; pages: "658-671.e7"
COMMENT     Sequence Label: pCW57.1-DUXB-CA
FEATURES             Location/Qualifiers
     source          1..8819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             52..96
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    309..426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        480..794
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    488..506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    524..542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    559..577
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    595..613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    631..649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    666..684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    702..720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     767..791
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(1860..1984)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2002..2019
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2029..2539
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2458..2476
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2549..3145
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2549..2568)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3045..3065
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3146..3199
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3200..3817,3818..3823,3824..3859,3860..3862,3863..3898,
                     3899..3901,3902..3937,3938..3946)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3986..4006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4404..4415)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4620..4853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4925..5046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4962..4981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5016..5035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5086..5221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5221..5292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5293..5361,5362..6153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5511..5530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6324..6912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6813..6832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7066..7083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7200..7221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7236..7241,7242..7259,7260..7266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7274..7290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7279..7301
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7298..7314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7298..7314
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7333..7353
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7335..7353
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7381..7607
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7608..7788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7835..7960
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8453..8686
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
       61 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
      121 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
      181 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
      241 cagacccacc tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc
      301 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      361 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      421 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcgaat cgatatgtcg
      481 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      541 gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      601 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      661 gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
      721 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
      781 accgtcagat cgcctggaga attggctagc ccaccatgaa tctggaaggc acgagtggag
      841 gaatcctgca aaaagagttt tggagaaatc gaatacagta caatcaatct caaaaagata
      901 tactccaatc ctggttccaa catgatccgt tccctgacaa ggctgctcgc gagcaactcg
      961 cgaaggagat tggggttccg gagagtaaca tccaggtctg gtttaaaaac tatcgcgtca
     1021 aacagcgcaa gctggattat aaatgcttca gcgagaagga ccaaactcag gggcatgacc
     1081 aatcccaaca cttgacccag gaatatctcc ctaaagaagc ccgccaaaaa caaaccttca
     1141 taacctggac tcagaagaat agacttgtac aagcattcga gcgaaaccca tttccagaca
     1201 ttgccacgcg caaaaagctc gccgagcaaa ccggactcca agagtccaga atccaaatgt
     1261 ggttccaaaa acaacgctct ctctacttga agaagtccag aatggaacct atgaatctgc
     1321 ttgtagatga cccgaacgag cgccccgatg cgacagtagg atggcaccct ataaatctgt
     1381 ttctgccgac ggatagtagc cactatttta gctgttctca ctcttcctca gggcacgaga
     1441 ctcttccccc agttttgccc agtacacagg ctccttggga tccttttaga tttcacgtat
     1501 cccaagggcc taatgtgatg attatgcagc caactcaagc agttcaagaa ggcgagaaat
     1561 cagaccaacc actgattatc cccaaccatc tgcttacact gcccattctc actaaggatc
     1621 ttgatacccc gacgccgttt tggcttcaat atcaagagga gcaccagaat cacaaggaac
     1681 acagtggaag cggcgttccc caagttaagt ctcacagcca acccgaaccc gagcataggg
     1741 aacaacagcc tcttaacctc ggtcagtttg atatctccaa cattcttcaa cgatgggacg
     1801 aaatctgtca agccttgttg gcggagtggg aaccgctgaa gggcacccat tgagtcgacc
     1861 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     1921 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     1981 tggtttagta atgaaccggt ccaccaccac caccaccact aaggatccgg ggttggggtt
     2041 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2101 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2161 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2221 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2281 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2341 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2401 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2461 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2521 caccgacctc tctccccagc aattcaccat gaccgagtac aagcccacgg tgcgcctcgc
     2581 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2641 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2701 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2761 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2821 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2881 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2941 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     3001 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     3061 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     3121 gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt
     3181 tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct
     3241 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     3301 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     3361 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     3421 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     3481 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     3541 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     3601 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     3661 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     3721 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     3781 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     3841 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     3901 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg
     3961 atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4021 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4081 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4141 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4201 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4261 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4321 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4381 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4441 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4501 ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag
     4561 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4621 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     4861 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4921 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5041 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5221 ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5281 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5341 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5401 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5461 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5521 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5581 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5641 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5701 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5761 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5821 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5881 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5941 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6001 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6061 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6121 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6181 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6241 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6961 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7021 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7081 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7141 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7201 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7261 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7321 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     7381 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     7441 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     7501 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     7561 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     7621 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7681 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7741 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     7801 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     7861 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7921 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7981 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8041 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8101 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8161 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8221 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     8281 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     8341 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8401 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8461 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8521 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8581 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8641 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8701 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8761 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaat
//
