LOCUS       sequence-244369-       11866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NOTCH2NLB full length under CAG promoter 
            with EGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 11866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP
FEATURES             Location/Qualifiers
     source          1..11866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3185..4201
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4125..4147
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4209..4228
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4264..4283
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4264..4282
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4297..4315
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4297..4314
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5181..5198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(5228..5802,5803..5805,5806..5814)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5395..5412)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5622..5641
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5815..5817,5818..5820,5821..6534)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5815..5817,5818..5820,5821..6531)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5860..5881)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6121..6140)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6468..6489
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6558..7146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6611..6631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7029..7040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7149..7165)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7150..7166)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7671..7851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7877..7894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7883..8107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8153..8581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8240..8259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8450..8471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8590..8610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8595..8924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8775..8910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8837..8856
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8972..9019
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9038..9412
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9542..9663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9579..9598)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9633..9652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9712..9728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9712..9728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9725..9747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9736..9752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9760..9766,9767..9784,9785..9790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9805..9826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9943..9960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10114..10702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10194..10213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10873..11664,11665..11733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11496..11515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11734..11838)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsggggg
     3061 gsggggsggg gsgrggsggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa
     3121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3181 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3241 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3301 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3361 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3421 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3481 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3541 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3601 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3661 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3721 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3781 ggcggggccg ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc
     3841 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     3901 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     3961 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     4021 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     4081 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4141 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     4201 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctcga
     4261 gcgtaatacg actcactata gggagaccca agcttgattt aggtgacact atagaataca
     4321 agctacttgt tctttttgca ggatccgacc gagagatgcc cgccctgcgc cccgctctgc
     4381 tgtgggcgct gctggcgctc tggctgtgct gcgcgacccc cgcgcatgca ttgcagtgtc
     4441 gagatggcta tgaaccctgt gtaaatgaag gaatgtgtgt tacctaccac aatggcacag
     4501 gatactgcaa atgtccagaa ggcttcttgg gggaatattg tcaacatcga gacccctgtg
     4561 agaagaaccg ctgccagaat ggtgggactt gtgtggccca ggccatgctg gggaaagcca
     4621 cgtgccgatg tgcctcaggg tttacaggag aggactgcca gtactcgaca tctcatccat
     4681 gctttgtgtc tcgaccttgc ctgaatggcg gcacatgcca tatgctcagc cgggatacct
     4741 atgagtgcac ctgtcaagtc gggtttacag gtaaggagtg ccaatggacc gatgcctgcc
     4801 tgtctcatcc ctgtgcaaat ggaagtacct gtaccactgt ggccaaccag ttctcctgca
     4861 aatgcctcac aggcttcaca gggcagaagt gtgagactga tgtcaatgag tgtgacattc
     4921 caggacactg ccagcatggt ggcatctgcc tcaacctgcc tggttcctac cagtgccagt
     4981 gccttcaggg cttcacaggc cagtactgtg acagcctgta tgtgccctgt gcaccctcgc
     5041 cttgtgtcaa tggaggcacc tgtcggcaga ctggtgactt cacttttgag tgcaactgcc
     5101 ttccagaaac agtgagaaga ggaacagagc tctgggaaag agacagggaa gtctggaatg
     5161 gaaaagaaca cgatgagaat caccaccatc atcaccacta gttaattaag ctagcgttaa
     5221 cgatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5281 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5341 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5401 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5461 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5521 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5581 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5641 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5701 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5761 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
     5821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     6001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     6061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     6121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     6301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     6361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaaattcg
     6541 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6961 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7081 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7141 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     7201 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7261 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7381 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     7441 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     7501 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     7561 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     7621 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     7681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     7861 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7921 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7981 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8041 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8101 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     8161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8581 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     8641 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     8701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     8761 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8821 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     8881 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     8941 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     9001 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     9061 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     9121 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     9181 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     9241 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     9301 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     9361 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     9421 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     9481 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     9541 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9661 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     9721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9841 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
