LOCUS       sequence-24438-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  TITLE     T4 Lysozyme Plasmids - Tray 5
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P22 Lysozyme WT
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             479..496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(549..567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     gap             589..618
                     /estimated_length=30
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgatgcaa atcagcagta acggaatcac
       61 cagattaaaa cgtgaagaag gtgagagact aaaagcctat tcagatagca gggggatacc
      121 aaccattggg gttgggcata ccggaaaagt ggatggtaat tctgtcgcat cagggatgac
      181 aatcaccgcc gaaaaatctt ctgaactgct taaagaggat ttgcagtggg ttgaagatgc
      241 gataagtagt cttgttcgcg tcccgctaaa tcagaaccag tatgatgcgc tatgtagcct
      301 gatattcaac ataggtaaat cagcatttgc cggctctacc gttcttcgcc agttgaattt
      361 aaagaattac caggcagcag cagatgcttt cctgttatgg aaaaaagctg gtaaagaccc
      421 tgatattctc cttccacgga ggcggcgaga aagagcgctg ttcttatcgt gactcgagca
      481 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaann nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnan gancnnnnnn ccggattggc gaatgggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgncctancg nccnntnctt t
//
