LOCUS       sequence-244381-        2300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein 
            kinase II alpha (T305D/T306D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Chang JY, Nakahata Y, Hayano Y, Yasuda R
  TITLE     Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in 
            single dendritic spines.
  JOURNAL   Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z.
  PUBMED    31239443
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-25"; volume: "10(1):2784. doi"; pages: " 
            10.1038/s41467-019-10694-z"
COMMENT     Sequence Label: pCAG-mEGFP-CaMKIIa (T305D/T306D)
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(56..58,59..61,62..772)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(101..122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(362..381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     709..730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2257..2276)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgctagcg ccaccatggt
       61 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      241 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      481 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      541 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      661 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
      781 cagatctcga gctatggcta ccatcacctg cacccgattc acggaagagt accagctctt
      841 cgaggaactg ggaaagggag ccttctccgt ggtgcgcagg tgtgtgaagg tgctggctgg
      901 ccaggagtat gctgccaaga ttatcaacac caagaagctc tcagccagag atcaccagaa
      961 gttggaacgc gaggcccgca tctgccgctt gttgaagcac cccaatatcg tccgactcca
     1021 tgacagcatc tccgaggagg ggcaccacta ccttatcttc gatctggtca ctggtgggga
     1081 gctgttcgaa gacattgtgg cccgggagta ttacagtgag gctgatgcca gccactgtat
     1141 ccagcagatc ctggaggctg tgctacactg tcaccagatg ggggtggtgc atcgcgacct
     1201 gaagcctgag aatctgttgc tggcttcgaa gctcaagggt gctgcggtga agctggcaga
     1261 ctttggcctg gccatagagg ttgagggaga gcagcaggca tggtttgggt tcgcagggac
     1321 acctggatac ctctccccag aagtgctgcg gaaggaccca tacgggaagc ctgtggacct
     1381 gtgggcctgt ggcgtcatcc tgtatatctt gctggttggg tatcccccat tctgggatga
     1441 ggaccagcac cgcctgtacc agcagatcaa agctggtgcc tacgatttcc catcaccaga
     1501 atgggacacc gtcaccccgg aagccaagga tctgatcaat aagatgctga ccatcaaccc
     1561 gtccaaacgc atcacggccg ctgaggctct caagcacccc tggatctcgc accgctccac
     1621 tgtggcctcc tgcatgcaca gacaggagac cgtggactgc ctgaagaagt tcaatgccag
     1681 gaggaaactg aagggagcca tcctcgacga tatgctggcc accaggaact tctccggagg
     1741 gaagagtgga ggaaacaaga agaatgatgg cgtgaaggaa tcctctgaga gcaccaacac
     1801 caccatcgag gatgaagaca ccaaagtgcg caaacaggaa attatcaaag tgacagagca
     1861 gctgatcgaa gccataagca atggagactt tgaatcctac acgaagatgt gcgaccctgg
     1921 aatgacagcc tttgaaccgg aggccctggg gaacctggtc gagggcctgg actttcatcg
     1981 attctatttt gaaaacctgt ggtcccggaa cagcaagccc gtgcacacca ccatcctgaa
     2041 ccctcacatc cacctgatgg gtgacgagtc agcctgcatc gcctacatcc gcatcactca
     2101 gtacctggat gcgggtggca tcccccgcac ggcccagtca gaggagaccc gtgtctggca
     2161 ccgcagggat ggaaaatggc agatcgtcca cttccacaga tctggggcgc cctccgtcct
     2221 gccccattga gcggccgcga ctctagagtc gacggatctt tttccctctg ccaaaaatta
     2281 tggggacatc atgaagcccc
//
