LOCUS       sequence-244384-        9163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal 3xFLAG tag. Gateway destination vector for lentivirus 
            production and stable transduction in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Agrotis A, Pengo N, Burden JJ, Ketteler R
  TITLE     Redundancy of human ATG4 protease isoforms in autophagy and 
            LC3/GABARAP processing revealed in cells.
  JOURNAL   Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.
  PUBMED    30661429
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2019 Jan 21. doi: 10.1080/15548627.2019.1569925."
COMMENT     Sequence Label: pLVpuro-CMV-N-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    899..920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(935..940,941..958,959..965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    973..989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     978..1000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     997..1013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     997..1013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1032..1052
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1034..1052
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1080..1306
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1307..1487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1534..1659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2152..2385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2570..2614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2912..2932
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3221..3424
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3375..3395
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3469..3487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3505..3514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3514..3579
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3583..3707
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3732..3737,3738..3755,3756..3762)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3816..4475
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3882..3901)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4817..5122
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5001..5020
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5163..5287)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5336..5453
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5502..6012
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5931..5949
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6034..6633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6034..6053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6530..6550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6761..6994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7066..7187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7103..7122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7157..7176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7227..7362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7383..7401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7411..7428)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7411..7427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7420..7442)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7569..8024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7656..7675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7866..7887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8050..8154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8155..8223,8224..9015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8373..8392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     1021 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     1081 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     1141 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     1201 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     1261 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     1321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2761 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2821 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
     2881 agactagcct cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata
     2941 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3001 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     3061 tttaaaatta tgttttaaaa tggactatca tatgccaagt acgcccccta ttgacgtcaa
     3121 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3181 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3241 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3301 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     3361 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3421 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     3481 ctatagggag acccaagctg gctagccacc atggactaca aagaccatga cggtgattat
     3541 aaagatcatg acatcgatta caaggatgac gatgacaagc ttacaagttt gtacaaaaaa
     3601 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     3661 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     3721 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     3781 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     3841 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     3901 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     3961 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     4021 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     4081 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     4141 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     4201 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     4261 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     4321 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     4381 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     4441 aacagtactg cgatgagtgg cagggcgggg cgtaaacgca tggatccggc ttactaaaag
     4501 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     4561 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     4621 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     4681 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     4741 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     4801 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     4861 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     4921 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     4981 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     5041 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     5101 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     5161 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5221 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5281 aagtggtgtg attcgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     5341 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     5401 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     5461 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
     5521 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     5581 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     5641 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     5701 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     5761 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     5821 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     5881 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     5941 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     6001 ctctctcccc agggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc
     6061 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6121 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6181 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6241 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6301 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6361 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6421 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6481 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6541 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6601 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     6661 accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca
     6721 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6781 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6961 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7021 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7201 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7381 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7441 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7501 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7561 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7621 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7681 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7741 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7801 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7861 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7921 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7981 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     8041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccg
//
