LOCUS       sequence-244399-       10155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human RET in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Xing S, Smanik PA, Oglesbee MJ, Trosko JE, Mazzaferri EL, Jhiang SM
  TITLE     Characterization of ret oncogenic activation in MEN2 inherited 
            cancer syndromes.
  JOURNAL   Endocrinology. 1996 May;137(5):1512-9. doi: 
            10.1210/endo.137.5.8612479.
  PUBMED    8612479
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1210/endo.137.5"; journalName: "Endocrinology"; date: "1996-05";
            volume: "137"; issue: "5"; pages: "1512-9"
COMMENT     Sequence Label: RET-WT
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5710..5728)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5711..5728)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5748..5765)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5754..5978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6024..6452
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6111..6130)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6321..6342
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6461..6481)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6466..6795
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6646..6781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6708..6727
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6862..7656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6916..6935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7526..7545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7830..7951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7867..7886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7921..7940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8000..8016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8000..8016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8013..8035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8024..8040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8048..8054,8055..8072,8073..8078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8093..8114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8231..8248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8402..8990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8482..8501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9161..9952,9953..10021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9784..9803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10022..10126)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttgccc
      901 cggaacgtgc gtcgcgcccc cagtgtccgt cgcgtccgcc gcgccccggg cggggatggg
      961 gcggccagac tgagcgccgc acccgccatc cagacccgcc ggccctagcc gcagtccctc
     1021 cagccgtggc cccagcgcgc acgggcgatg gcgaaggcga cgtccggtgc cgcggggctg
     1081 cgtctgctgt tgctgctgct gctgccgctg ctaggcaaag tggcattggg cctctacttc
     1141 tcgagggatg cttactggga gaagctgtat gtggaccagg cggccggcac gcccttgctg
     1201 tacgtccatg ccctgcggga cgcccctgag gaggtgccca gcttccgcct gggccagcat
     1261 ctctacggca cgtaccgcac acggctgcat gagaacaact ggatctgcat ccaggaggac
     1321 accggcctcc tctaccttaa ccggagcctg gaccatagct cctgggagaa gctcagtgtc
     1381 cgcaaccgcg gctttcccct gctcaccgtc tacctcaagg tcttcctgtc acccacatcc
     1441 cttcgtgagg gcgagtgcca gtggccaggc tgtgcccgcg tatacttctc cttcttcaac
     1501 acctcctttc cagcctgcag ctccctcaag ccccgggagc tctgcttccc agagacaagg
     1561 ccctccttcc gcattcggga gaaccgaccc ccaggcacct tccaccagtt ccgcctgctg
     1621 cctgtgcagt tcttgtgccc caacatcagc gtggcctaca ggctcctgga gggtgagggt
     1681 ctgcccttcc gctgcgcccc ggacagcctg gaggtgagca cgcgctgggc cctggaccgc
     1741 gagcagcggg agaagtacga gctggtggcc gtgtgcaccg tgcacgccgg cgcgcgcgag
     1801 gaggtggtga tggtgccctt cccggtgacc gtgtacgacg aggacgactc ggcgcccacc
     1861 ttccccgcgg gcgtcgacac cgccagcgcc gtggtggagt tcaagcggaa ggaggacacc
     1921 gtggtggcca cgctgcgtgt cttcgatgca gacgtggtac ctgcatcagg ggagctggtg
     1981 aggcggtaca caagcacgct gctccccggg gacacctggg cccagcagac cttccgggtg
     2041 gaacactggc ccaacgagac ctcggtccag gccaacggca gcttcgtgcg ggcgaccgta
     2101 catgactata ggctggttct caaccggaac ctctccatct cggagaaccg caccatgcag
     2161 ctggcggtgc tggtcaatga ctcagacttc cagggcccag gagcgggcgt cctcttgctc
     2221 cacttcaacg tgtcggtgct gccggtcagc ctgcacctgc ccagtaccta ctccctctcc
     2281 gtgagcagga gggctcgccg atttgcccag atcgggaaag tctgtgtgga aaactgccag
     2341 gcgttcagtg gcatcaacgt ccagtacaag ctgcattcct ctggtgccaa ctgcagcacg
     2401 ctaggggtgg tcacctcagc cgaggacacc tcggggatcc tgtttgtgaa tgacaccaag
     2461 gccctgcggc ggcccaagtg tgccgaactt cactacatgg tggtggccac cgaccagcag
     2521 acctctaggc aggcccaggc ccagctgctt gtaacagtgg aggggtcata tgtggccgag
     2581 gaggcgggct gccccctgtc ctgtgcagtc agcaagagac ggctggagtg tgaggagtgt
     2641 ggcggcctgg gctccccaac aggcaggtgt gagtggaggc aaggagatgg caaagggatc
     2701 accaggaact tctccacctg ctctcccagc accaagacct gccccgacgg ccactgcgat
     2761 gttgtggaga cccaagacat caacatttgc cctcaggact gcctccgggg cagcattgtt
     2821 gggggacacg agcctgggga gccccggggg attaaagctg gctatggcac ctgcaactgc
     2881 ttccctgagg aggagaagtg cttctgcgag cccgaagaca tccaggatcc actgtgcgac
     2941 gagctgtgcc gcacggtgat cgcagccgct gtcctcttct ccttcatcgt ctcggtgctg
     3001 ctgtctgcct tctgcatcca ctgctaccac aagtttgccc acaagccacc catctcctca
     3061 gctgagatga ccttccggag gcccgcccag gccttcccgg tcagctactc ctcttccggt
     3121 gcccgccggc cctcgctgga ctccatggag aaccaggtct ccgtggatgc cttcaagatc
     3181 ctggaggatc caaagtggga attccctcgg aagaacttgg ttcttggaaa aactctagga
     3241 gaaggcgaat ttggaaaagt ggtcaaggca acggccttcc atctgaaagg cagagcaggg
     3301 tacaccacgg tggccgtgaa gatgctgaaa gagaacgcct ccccgagtga gcttcgagac
     3361 ctgctgtcag agttcaacgt cctgaagcag gtcaaccacc cacatgtcat caaattgtat
     3421 ggggcctgca gccaggatgg cccgctcctc ctcatcgtgg agtacgccaa atacggctcc
     3481 ctgcggggct tcctccgcga gagccgcaaa gtggggcctg gctacttggg cagtggaggc
     3541 agccgcaact ccagctccct ggaccacccg gatgagcggg ccctcaccat gggcgacctc
     3601 atctcatttg cctggcagat ctcacagggg atgcagtatc tggccgagat gaagctcgtt
     3661 catcgggact tggcagccag aaacatcctg gtagctgagg ggcggaagat gaagatttcg
     3721 gatttcggct tgtcccgaga tgtttatgaa gaggattcct acgtgaagag gagccagggt
     3781 cggattccag ttaaatggat ggcaattgaa tccctttttg atcatatcta caccacgcaa
     3841 agtgatgtat ggtcttttgg tgtcctgctg tgggagatcg tgaccctagg gggaaacccc
     3901 tatcctggga ttcctcctga gcggctcttc aaccttctga agaccggcca ccggatggag
     3961 aggccagaca actgcagcga ggagatgtac cgcctgatgc tgcaatgctg gaagcaggag
     4021 ccggacaaaa ggccggtgtt tgcggacatc agcaaagacc tggagaagat gatggttaag
     4081 aggagagact acttggacct tgcggcgtcc actccatctg actccctgat ttatgacgac
     4141 ggcctctcag aggaggagac accgctggtg gactgtaata atgcccccct ccctcgagcc
     4201 ctcccttcca catggattga aaacaaactc tatggcatgt cagacccgaa ctggcctgga
     4261 gagagtcctg taccactcac gagagctgat ggcactaaca ctgggtttcc aagatatcca
     4321 aatgatagtg tatatgctaa ctggatgctt tcaccctcag cggcaaaatt aatggacacg
     4381 tttgatagtt aacatttctt tgtgaaaggt aatggactca caaggggaag aaacatgctg
     4441 agaatggaaa gtctaccggc cctttctttg tgaacgtcac attggccgag ccgtgttcag
     4501 ttcccaggtg gcagactcgt ttttggtagt ttgttttaac ttccaaggtg gttttacttc
     4561 tgatagccgg tgattttccc tcctagcaga catgccacac cgggtaagag ctctgagtct
     4621 tagtggttaa gcattccttt ctcttcagtg cccagcagca cccagtgttg gtctgtgtcc
     4681 atcagtgacc accaacattc tgtgttcaca tgtgtgggtc caacacttac tacctggtgt
     4741 atgaaattgg acctgaactg ttggattttt ctagttgccg ccaaacaagg caaaaaaatt
     4801 taaacatgaa gcacacacac aaaaaaggca gtaggaaaaa tgctggccct gatgacctgt
     4861 ccttattcag aatgagagac tgcggggggg gcctgggggt agtgtcaatg cccctccagg
     4921 gctggagggg aagaggggcc ccgaggatgg gcctgggctc agcattcgag atcttgagaa
     4981 tgattttttt ttaatcatgc aacctttcct taggaagaca tttggttttc atcatgatta
     5041 agatgattcc tagatttagc acaatggaga gattccatgc catctttact atgtggatgg
     5101 tggtatcagg gaagagggct cacaagacac atttgtcccc cgggcccacc acatcatcct
     5161 cacgtgttcg gtactgagca gccactaccc ctgatgagaa cagtatgaag aaagggggct
     5221 gttggagtcc cagaattgct gacagcagag gctttgctgc tgtgaatccc acctgccacc
     5281 agcctgcagc acaccccaca gccaagtaga ggcgaaagca gtggctcatc ctacctgtta
     5341 ggagcaggta gggcttgtac tcactttaat ttgaatctta tcaacttact cataaaggga
     5401 caggctagct agctgtgtta gaagtagcaa tgacaatgac caaggactgc tacacctctg
     5461 attacaattc tgatgtgaaa aagatggtgt ttggctctta tagagcctgt gtgaaaggcc
     5521 catggatcag ctcttcctgt gtttgtaatt taatgctgct acaagatgtt tctgtttctt
     5581 agattctgac catgactcat aagcttggta ccgagctcgg atccactagt aacggccgcc
     5641 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     5701 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     5761 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5821 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5881 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5941 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6001 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6061 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6121 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6181 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6241 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6301 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6361 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6421 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6541 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6601 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6661 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6721 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6781 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6841 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6901 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6961 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7021 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7081 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7141 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7201 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7261 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7321 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7381 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7441 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7501 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7561 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7621 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     7681 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     7741 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     7801 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     7861 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7921 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     7981 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8041 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8821 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10141 aagtgccacc tgacg
//
