LOCUS       sequence-24440-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: AF0837(1-127)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(476..494)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      490..537
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(664..683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagaaggaga tatacatatg cagattgttc ccgtttaacc tgagggataa cgagggtgag
       61 ataaagctaa tccccgaaac gctcgacgac ctctggcatc tccgcttcat aatcgagaag
      121 ggagatgtgg ttttcgcaac tacgaaaagg gcgagccaga gcagcgacaa gctgaggagc
      181 gataaggaga tggtcacggt gaggctcggc atcgaggtgg agaaggtgga gtttcacagg
      241 ttcgccaacc gtctcagggt ttctgggaag atagttgccg ggattgagga gtccggatac
      301 cacactctga atatcactgt gggcaaggag ctgagcataa ttaaaaagtg gaagccagag
      361 cagcttgaaa gactgaggag ggccgttgag gattccaacc tcgagcacca ccaccaccac
      421 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      481 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      541 aagngngatt ntttctnccn ntatnggccg aatggcacgc gccctgtngc gncgcnttaa
      601 ncgcgncggg tgtggtggnt tcntgcagcg ttgaccgtta catcggcnag cgccctacgc
      661 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cccggctttc cccgtcagct
      721 ctaatcgggg ctcccttagg ttcnatttag tnttncgcnc tcacccaaaa ctntagggat
      781 gtccnatggc ncccctgaaa cgtttcnctt gnntgaccng tttaatgact ttcatgaact
      841 ccct
//
