LOCUS       sequence-244402-        5655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal myc-tagged mouse Pex19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Covill-Cooke C, Toncheva VS, Drew J, Birsa N, Lopez-Domenech G, 
            Kittler JT
  TITLE     Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, 
            Miro1 and Miro2.
  JOURNAL   EMBO Rep. 2020 Feb 5;21(2):e49865. doi: 10.15252/embr.201949865. 
            Epub 2020 Jan 2.
  PUBMED    31894645
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2020-02-5"; volume: "21(2):e49865. doi"; pages: " 
            10.15252/embr.201949865. Epub 2020 Jan 2"
COMMENT     Sequence Label: myc-Pex19
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      968..977
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1900..1909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1913..2047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1950..1969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2004..2023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2120..2477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2120..2311
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2143..2163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2328..2463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2497..2514)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2497..2513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2726..3181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2813..2832)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3023..3044
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3198..3217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3352..3374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3427..3445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3513..3617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3618..3686,3687..4478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3836..3855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4649..5237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5138..5157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5391..5408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(5561..5566,5567..5584,5585..5591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5599..5615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5604..5626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5623..5639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5623..5639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tagggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaaggcg gattccccgt
      721 gccaagagtg acgtaagtac cgcgtataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attggccacc atggagcaga agctgatctc cgaggaggac
      961 ctgggatcca tggcggctgc tgaggaaggt tgcggtgttg gggtcgaaga cgaccgggaa
     1021 ctggaggagc ttctggaaag tgctcttgat gatttcgaca aagccaaacc ctccccagaa
     1081 catgctccga ccatctcggc tcccgacgct tcaggacccc agaagagagc gccaggagat
     1141 actgccaaag atgctctctt cgcctcccaa gagaaatttt tccaggaact gtttgacagt
     1201 gagctggctt cccaagctac tgcggagttt gagaaggcaa tgaaggagct ggctgaggaa
     1261 gagccccatc tggtggagca gttccagaag ctctcagagg cagctgggag agtgggcagc
     1321 gatgcaagtt ctcagcaaga gtttacttct tgcctaaagg agacgttaag tggcctggcc
     1381 aaaaacgcca ctgagctgca gaactcgggc atgtctgaag aggagctgat gaaagccatg
     1441 gaagggctgg gcatggatga gggggatggg gaagcgagca ttctccccat catgcagagc
     1501 atcatgcaga acctcctgtc taaggatgtg ctgtacccat ccctgaagga gatcacagaa
     1561 aagtatccag aatggctcca gagtcaccag gactccactc ctcccgagca gtttgagaag
     1621 taccagcagc agcacagcgt catggtcaaa atctgtgagc agtttgaggc cgagacgccc
     1681 acagacagcg aggctactca gagggctcgc tttgaggcca tgctagatct catgcagcag
     1741 ttacaggcct tgggccatcc tccaaaagag ctggctgggg agatgcctcc tggcctcaac
     1801 tttgacctgg atgctctcaa tctgtcgggc cccccaggtg ctaatggcga acagtgtctg
     1861 atcatggaat tcctgcagaa gcttgtcgac cctgcagggg ccgccatggc ccaacttgtt
     1921 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1981 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2041 ctggatcgat cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag
     2101 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     2161 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2221 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2281 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2401 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2461 gcctaggctt ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt tacaacgtgg
     2521 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccccttcgc
     2581 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgtagcct
     2641 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2701 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2761 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2821 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2881 gggctccctt tagggttgcg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2941 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3001 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3061 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3121 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3181 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca actccgctat
     3241 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     3301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagtat tcttgaagac
     3421 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3481 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3601 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3661 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3721 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3781 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3841 gtggcgcggt attatcccgt gatgacgccg ggcaagagca actcggtcgc cgcatacact
     3901 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3961 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4021 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4081 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4141 agcgtgacac cacgatgcca gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4201 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4261 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4321 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4381 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4441 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4501 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5041 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5221 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5341 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5401 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5461 ttcattaatc cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5521 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
     5581 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5641 atgattacga attaa
//
