LOCUS       sequence-244407-       13951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from
            promoter PLhrtO, ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 13951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM534
FEATURES             Location/Qualifiers
     source          1..13951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             89..100
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             107..1549
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1562..2485
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2534..3616
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3631..4614
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4793..5905
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     RBS             6018..6029
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6036..7478
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7491..8414
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             8463..9545
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             9560..10543
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             10722..11834
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      11894..11980
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(12145..12733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12225..12244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(12821..12915)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(12946..13740)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13057..13076)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13667..13686
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13919..13937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagatgc tataaatgac acagtgtcat ttgacaaaat gacacagtgt catgatactg
       61 agcacatcag caggacgcac tgaccattaa agaggagaaa ggtaccatga ctaaaaaaat
      121 ttcattcatt attaacggcc aggttgaaat ctttcccgaa ggtgatgatt tagtgcaatc
      181 cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc atgtaaaaaa
      241 cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca attttctcta
      301 tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat acattcgtga
      361 cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca attggatatc
      421 tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac ttggttctcg
      481 ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt ttccgaaagg
      541 taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt ctatattacg
      601 cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc cttttaccgc
      661 taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa cgcgctcttt
      721 atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa ttatgcaaca
      781 tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg tagagcatgc
      841 gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca ttatcgataa
      901 tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt tttacgatca
      961 gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg aggaatttaa
     1021 gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg ccaaaaaaga
     1081 ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcttgtttg ctggattaaa
     1141 agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag gtgtggaatt
     1201 taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg agcaaatatt
     1261 gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg agtcatcatt
     1321 taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag caggaatgaa
     1381 taacatattt cgagttggtg gatctcatga cggaatgcga ccgttgcaac gattagtgac
     1441 atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg ttgaaataga
     1501 acagactcga ttcctggaag aagataagtt ccttgtattt gtcccataat aggtaaaaag
     1561 tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa
     1621 aaaaattcat gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat
     1681 tattattgcg tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt
     1741 atcgcggaat ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc
     1801 agggacaatt gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga
     1861 ttggttaact acacgaaaaa taaataactt cggtatgttg gcttcaagct tatctgcgcg
     1921 gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt
     1981 tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat
     2041 taatgaattg ccgaataatc tagattttga aggccataaa ttgggtgctg aagtctttgc
     2101 gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta
     2161 tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga
     2221 agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg
     2281 aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac
     2341 gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc
     2401 gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga
     2461 aaatcaagca atttctctgt cttaaaatct attgagatat tctatcactc aaatagcaat
     2521 ataaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc
     2581 tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt
     2641 tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta
     2701 tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat
     2761 tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat
     2821 gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt
     2881 attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa
     2941 gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt
     3001 aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc
     3061 agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat
     3121 aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg
     3181 gcacgatatt cataatatcg accattgctt atcatatata acatctgtag atcatgactc
     3241 aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa
     3301 tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca ataaagggca
     3361 gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg attacagtta
     3421 cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa aagacattga
     3481 tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag tagacgaaat
     3541 tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag aaaaacaacg
     3601 ttcgctatta tattagctaa ggagaaagaa atgaaatttg gattgttctt ccttaacttc
     3661 atcaattcaa caactgttca agaacaaagt atagttcgca tgcaggaaat aacggagtat
     3721 gttgataagt tgaattttga acagatttta gtgtatgaaa atcatttttc agataatggt
     3781 gttgtcggcg ctcctctgac tgtttctggt tttctgctcg gtttaacaga gaaaattaaa
     3841 attggttcat taaatcacat cattacaact catcatcctg tccgcatagc ggaggaagct
     3901 tgcttattgg atcagttaag tgaagggaga tttattttag ggtttagtga ttgcgaaaaa
     3961 aaagatgaaa tgcatttttt taatcgcccg gttgaatatc aacagcaact atttgaagag
     4021 tgttatgaaa tcattaacga tgctttaaca acaggctatt gtaatccaga taacgatttt
     4081 tatagcttcc ctaaaatatc tgtaaatccc catgcttata cgccaggcgg acctcggaaa
     4141 tatgtaacag caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc
     4201 atctttaagt gggatgattc taatgatgtt agatatgaat atgctgaaag atataaagcc
     4261 gttgcggata aatatgacgt tgacctatca gagatagacc atcagttaat gatattagtt
     4321 aactataacg aagatagtaa taaagctaaa caagagacgc gtgcatttat tagtgattat
     4381 gttcttgaaa tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat aattgcagaa
     4441 aacgctgtcg gaaattatac ggagtgtata actgcggcta agttggcaat tgaaaagtgt
     4501 ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg atttgatgag ccaaaaaaat
     4561 gtaatcaata ttgttgatga taatattaag aagtaccaca tggaatatac ctaatagatt
     4621 tcgagttgca gcgaggcggc aagtgaacga atccccagga gcatagataa ctatgtgact
     4681 ggggtgagtg aaagcagcca acaaagcagc agcttgaaag atgaagggta taaaagagta
     4741 tgacagcagt gctgccatac tttctaatat tatcttgagg agtaaaacag gtatgacttc
     4801 atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag
     4861 cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga
     4921 tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa
     4981 agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa
     5041 gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg
     5101 cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg
     5161 ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa tagaattagt
     5221 caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt
     5281 ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa
     5341 aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc
     5401 gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg
     5461 agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct
     5521 gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca
     5581 gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca
     5641 gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc
     5701 tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc
     5761 agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg
     5821 cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag
     5881 cttaaccgaa gcgtttgata gttgaaagct tgatatcgaa ttccttcacc tcgagatgct
     5941 ataaatgaca cagtgtcatt tgacaaaatg acacagtgtc atgatactga gcacatcagc
     6001 aggacgcact gaccattaaa gaggagaaag gtaccatgac taaaaaaatt tcattcatta
     6061 ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc attaattttg
     6121 gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac attattgatt
     6181 gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat acggtagggc
     6241 aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac ttaaaaaaat
     6301 atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct atgattttat
     6361 gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc catatcatgg
     6421 atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac
     6481 atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc gcaattttaa
     6541 ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct aatgcattag
     6601 cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta tctgttatat
     6661 attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat gcggatgtta
     6721 ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg ccatcttatg
     6781 ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat cctgttgatt
     6841 tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag cgagcttgtt
     6901 tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag ttagcgttga
     6961 tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat tttgatgaaa
     7021 aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg
     7081 atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt aatcaaccac
     7141 ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg ccttatgttc
     7201 aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt aaatatcgag
     7261 atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat aacatatttc
     7321 gagttggtgg atctcatgac ggaatgcgac cgttgcaacg attagtgaca tatatttctc
     7381 atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa cagactcgat
     7441 tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt atggaaaatg
     7501 aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa aaaattcatg
     7561 tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt attattgcgt
     7621 ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta tcgcggaatg
     7681 gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca gggacaattg
     7741 atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat tggttaacta
     7801 cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg atagcttatg
     7861 caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa
     7921 gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt aatgaattgc
     7981 cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg agagattgtc
     8041 ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat cttgatatac
     8101 cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa gttatcacat
     8161 tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga agttcgcatg
     8221 acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg aaagccgcta
     8281 tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg tcatttgaac
     8341 atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa
     8401 tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata taaggactct
     8461 ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct caaacagagg
     8521 taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt gataccgtat
     8581 ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat gtcgctgctg
     8641 catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt gttcttccca
     8701 cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac
     8761 gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta ttcggcacag
     8821 atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag aatggcatga
     8881 cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta aaagtaaacc
     8941 ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga
     9001 ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata aatactaacg
     9061 aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg cacgatattc
     9121 ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca attaaagcga
     9181 aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat gctacgacta
     9241 tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag tggcgtgact
     9301 ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac gaaatcaatc
     9361 ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat gctacaggaa
     9421 tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt attgcttcca
     9481 tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt tcgctattat
     9541 attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca tcaattcaac
     9601 aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg ttgataagtt
     9661 gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc
     9721 tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa ttggttcatt
     9781 aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt gcttattgga
     9841 tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat
     9901 gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt gttatgaaat
     9961 cattaacgat gctttaacaa caggctattg taatccagat aacgattttt atagcttccc
    10021 taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat atgtaacagc
    10081 aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca tctttaagtg
    10141 ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg ttgcggataa
    10201 atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta actataacga
    10261 agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg ttcttgaaat
    10321 gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa acgctgtcgg
    10381 aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag
    10441 tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg taatcaatat
    10501 tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt cgagttgcag
    10561 cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg gggtgagtga
    10621 aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat gacagcagtg
    10681 ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca tatgttgata
    10741 aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc gatccattag
    10801 tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta
    10861 atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa gtagatgaca
    10921 atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag tttactcgct
    10981 tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc acgaatggtt
    11041 taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc tctgtgagtt
    11101 atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc aatttgggac
    11161 cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg gtggaattgt
    11221 tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa acattgaata
    11281 gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg ccatacttta
    11341 tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga gataaaagcc
    11401 tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg
    11461 atttcaatca tcttttattt gatactttca atctcagtga tattagtcag atccgagata
    11521 tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag cgtaaacatg
    11581 ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct gtacctgatg
    11641 gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca gcatttattg
    11701 ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc gtgctcgttg
    11761 aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc ttaaccgaag
    11821 cgtttgatag ttgaaagctt gatatcgaat tcctgcagcc cgggggatcc catggtacgc
    11881 gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
    11941 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg
    12001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    12061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    12121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    12181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    12241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    12301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    12541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    12601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12781 aaactcacgt taagggattt tggtcatgac tagtgcttgg attctcacca ataaaaaacg
    12841 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
    12901 ctatcaacag gagtccaagc gagctctcga accccagagt cccgctcaga agaactcgtc
    12961 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
    13021 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
    13081 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
    13141 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
    13201 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
    13261 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
    13321 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
    13381 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
    13441 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
    13501 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
    13561 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
    13621 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
    13681 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
    13741 gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct
    13801 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
    13861 cccagctggc aattccgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    13921 taggcgtatc acgaggccct ttcgtcttca c
//
