LOCUS       sequence-244413-        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a fluorecent protein with an RNA binding peptide: 
            YPet-PCP.Expression with constitutive E. coli RNAP promoter 
            (J23106), ribozyme RiboJ and RBS BBa_B0034..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, 
            Simmel FC
  TITLE     Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in
            a Cell-Free Gene Expression System.
  JOURNAL   Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 
            10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
  PUBMED    29564885
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.8b00526"; journalName: "Nano Lett"; date: 
            "2018-04-11- 11"; volume: "18"; issue: "4"; pages: "2650-2657"
COMMENT     Sequence Label: pSB1C3_YPet-PCP (y-plasmid)
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(65..653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(145..164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(836..930)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(951..1610)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1525..1544
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1772..1790
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1794..1837)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        1962..1996
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2085..2096
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    3280..3300
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3301..3358
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3382..3399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
       61 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      181 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      241 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      301 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      361 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      421 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      481 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      541 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      601 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      661 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      721 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      781 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
      841 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      901 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      961 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1021 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1081 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1141 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     1201 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1261 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1321 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1381 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1441 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1501 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1561 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1621 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1681 tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga
     1741 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     1801 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgagta agcccctcta
     1861 gaggaccacg catcgtgatg cctatgcgcg gtagtcccac cttgtccact agaatggaag
     1921 attggcacgt atcaagactt tggagtagta ccataacgcc gtttacggct agctcagtcc
     1981 taggtatagt gctagctagc gcagcgctca acgggtgtgc ttcccgttct gatgagtccg
     2041 tgaggacgaa agcgcctcta caaataattt tgtttaatca tgagaaagag gagaaaacta
     2101 gatgtctaaa ggtgaagaac tgtttacggg tgtcgtgccg attctggtcg agttggacgg
     2161 cgacgtgaac ggtcacaaat tcagcgtgag cggcgagggc gagggtgacg cgacgtacgg
     2221 taagctgact ctgaagctgc tgtgcaccac gggtaaattg ccggttccgt ggccgaccct
     2281 ggtcacgacg ctgggttatg gtgtacaatg ttttgcacgc tatccggacc acatgaaaca
     2341 gcacgatttc ttcaagagcg cgatgccgga aggctatgtt caggaacgta ccatcttttt
     2401 caaagatgat ggtaattaca aaacccgcgc agaagtgaag ttcgagggtg acaccctggt
     2461 gaaccgtatt gagctgaagg gtattgactt caaggaagat ggcaatattc tgggtcacaa
     2521 actggagtac aactataaca gccataacgt ctacatcacc gcggataagc aaaaaaatgg
     2581 tatcaaagca aatttcaaga ttcgccacaa catcgaagat ggcggcgtgc aactggccga
     2641 tcattatcag cagaataccc caatcggtga cggtccggtg ctgttgccgg ataaccacta
     2701 cctgagctat caaagcgcgt tgttcaaaga cccgaatgaa aaacgtgacc acatggttct
     2761 gctggaattt ctgaccgctg cgggcatcac tgaaggcatg aatgaactgt acaagacgcg
     2821 tggtggcggc ggttcgatga gcaagactat cgttttgtcc gtcggcgagg ctacccgtac
     2881 cttgaccgaa attcaatcca ccgcggaccg tcaaattttt gaggaaaaag tcggtcctct
     2941 ggtgggtcgt ctgcgtctga ccgcgagcct gcgccagaac ggtgccaaaa cggcataccg
     3001 tgttaatctg aaactggatc aggccgacgt tgtggacagc ggtctgccga aagtccgcta
     3061 cacccaggtg tggagccacg atgtgacgat cgttgcgaat agcaccgaag cgagccgcaa
     3121 gagcctgtac gacctgacca agagcctggt ggcaacgtcc caagttgaag atctggttgt
     3181 taacctggtg ccgctgggtc gttaaagcat gccggaggaa acacagaaaa aagcccgcac
     3241 ctgacagtgc gggctttttt tttcgaccaa aggggtgcat actagtagcg gccgctgcag
     3301 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     3361 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3481 cgcaggaa
//
