LOCUS       sequence-244415-       11184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NOTCH2NL delta EGF repeats under CAG 
            promoter with EGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-de...
FEATURES             Location/Qualifiers
     source          1..11184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    93..210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        255..558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        560..837
                     /label=chicken beta-actin promoter
     intron          838..1854
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1778..1800
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1862..1881
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1917..1936
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1917..1935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1950..1968
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1950..1967
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2161..2178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(2199..2773,2774..2776,2777..2785)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2366..2383)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2593..2612
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2786..2788,2789..2791,2792..3505)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2786..2788,2789..2791,2792..3502)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2831..2852)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3092..3111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3439..3460
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3529..4117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3582..3602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4000..4011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4120..4136)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4121..4137)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4642..4822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4848..4865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4854..5078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5124..5552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5211..5230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5421..5442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5561..5581)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5566..5895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5746..5881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5808..5827
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5943..5990
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6009..6383
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6513..6634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6550..6569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6604..6623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6683..6699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6683..6699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6696..6718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6707..6723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6731..6737,6738..6755,6756..6761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6776..6797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6914..6931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7085..7673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7165..7184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7844..8635,8636..8704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8467..8486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8705..8809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8884..8903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9075..9454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9456..9654
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9609..9629
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9672..9852
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9899..10024
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10517..10750
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10935..10979
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
       61 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      121 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      181 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      241 gtttggttaa tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      301 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      361 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      421 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      481 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      541 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      601 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      661 ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs
      721 ggggsgrggc ggagaggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      781 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      841 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      901 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      961 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     1021 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     1081 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     1141 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1201 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1261 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1321 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1381 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1441 ccgcctcggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc
     1501 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1561 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1621 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1681 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1741 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1801 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1861 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct cgagcgtaat
     1921 acgactcact atagggagac ccaagcttga tttaggtgac actatagaat acaagctact
     1981 tgttcttttt gcaggatcgc caccatgccc gccctgcgcc ccgctctgct gtgggcgctg
     2041 ctggcgctct ggctgtgctg cgcgaccccc gcgcatgcaa ccggtgaaac agtgagaaga
     2101 ggaacagagc tctgggaaag agacagggaa gtctggaatg gaaaagaaca cgatgagaat
     2161 caccaccatc atcaccacta gctagcgtta acgatccgcc cctctccctc ccccccccct
     2221 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     2281 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     2341 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     2401 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     2461 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     2521 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     2581 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     2641 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     2701 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     2761 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt
     2821 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2881 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2941 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3001 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3061 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3121 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3181 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3241 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3301 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3361 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3421 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3481 cggcatggac gagctgtaca agtaaaattc gatatcaagc ttatcgataa tcaacctctg
     3541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3901 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4081 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     4141 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     4201 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     4261 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4321 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     4381 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     4441 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     4501 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     4561 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     4621 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4801 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5041 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5101 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5461 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5521 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5641 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5701 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5821 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5881 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5941 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     6001 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     6061 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     6121 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     6181 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     6241 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     6301 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     6361 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     6421 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6481 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6601 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6661 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6721 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6781 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6841 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7681 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7741 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7801 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7861 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7921 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7981 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8041 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8101 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8161 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8221 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8281 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8341 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8401 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8461 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8521 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8581 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8641 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8701 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8761 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8821 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8881 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8941 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     9001 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     9061 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     9121 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     9181 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     9241 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     9301 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     9361 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     9421 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     9481 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     9541 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     9601 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     9661 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9721 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9781 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9841 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9901 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9961 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    10021 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    10081 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    10141 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    10201 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    10261 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    10321 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
    10381 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10441 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10501 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10561 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    10621 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    10681 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    10741 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10801 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10861 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10921 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10981 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    11041 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    11101 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    11161 ccgaaggaat agaagaagaa ggtg
//
