LOCUS       sequence-244423-       11792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NOTCH2NL delta C terminus under CAG 
            promoter with EGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-del...
FEATURES             Location/Qualifiers
     source          1..11792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        288..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          871..1887
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1811..1833
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1895..1914
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1950..1969
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1950..1968
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1983..2001
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1983..2000
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2801..2818
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(2840..3414,3415..3417,3418..3426)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3007..3024)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3234..3253
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3427..3429,3430..3432,3433..4146)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3427..3429,3430..3432,3433..4143)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3472..3493)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3733..3752)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4080..4101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4170..4758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4223..4243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4641..4652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4761..4777)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4762..4778)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5283..5463
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5489..5506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5495..5719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5765..6193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5852..5871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6062..6083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6202..6222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6207..6536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6387..6522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6449..6468
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6584..6631
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6650..7024
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7154..7275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7191..7210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7245..7264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7324..7340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7324..7340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7337..7359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7348..7364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7372..7378,7379..7396,7397..7402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7417..7438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7555..7572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7726..8314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7806..7825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8485..9276,9277..9345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9108..9127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9346..9450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9525..9544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9716..10095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10097..10295
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10250..10270
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10313..10493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10540..10665
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11158..11391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11576..11620
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccagtttggt taatccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      601 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      661 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
      721 ccrggsgggg sggggsgggg sggggggsgg ggsggggsgr ggsggrgrgg kgcggcggca
      781 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      841 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      901 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      961 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1021 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
     1081 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     1141 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1201 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     1261 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1321 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     1381 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     1441 tggcggcagg tgggggtgcc gggcggggcg gggccgccyc gggccggggr gggctcgggg
     1501 grggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     1561 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     1621 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     1681 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     1741 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     1801 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     1861 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1921 catcattttg gcaaagaatt gctcgagcgt aatacgactc actataggga gacccaagct
     1981 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cggttaccac
     2041 catgcccgcc ctgcgccccg ctctgctgtg ggcgctgctg gcgctctggc tgtgctgcgc
     2101 gacccccgcg catgcattgc agtgtcgaga tggctatgaa ccctgtgtaa atgaaggaat
     2161 gtgtgttacc taccacaatg gcacaggata ctgcaaatgt ccagaaggct tcttggggga
     2221 atattgtcaa catcgagacc cctgtgagaa gaaccgctgc cagaatggtg ggacttgtgt
     2281 ggcccaggcc atgctgggga aagccacgtg ccgatgtgcc tcagggttta caggagagga
     2341 ctgccagtac tcgacatctc atccatgctt tgtgtctcga ccttgcctga atggcggcac
     2401 atgccatatg ctcagccggg atacctatga gtgcacctgt caagtcgggt ttacaggtaa
     2461 ggagtgccaa tggaccgatg cctgcctgtc tcatccctgt gcaaatggaa gtacctgtac
     2521 cactgtggcc aaccagttct cctgcaaatg cctcacaggc ttcacagggc agaagtgtga
     2581 gactgatgtc aatgagtgtg acattccagg acactgccag catggtggca tctgcctcaa
     2641 cctgcctggt tcctaccagt gccagtgcct tcagggcttc acaggccagt actgtgacag
     2701 cctgtatgtg ccctgtgcac cctcgccttg tgtcaatgga ggcacctgtc ggcagactgg
     2761 tgacttcact tttgagtgca actgccttcc agaactagcg caccaccatc atcaccacta
     2821 gtctagcgtt aacgatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     2881 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2941 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     3001 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     3061 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     3121 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     3181 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3241 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3301 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3361 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     3421 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3481 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3541 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3601 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3661 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3721 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3781 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3841 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3901 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3961 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4021 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4141 aagtaaaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4201 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4261 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4321 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4381 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4441 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4501 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4561 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4621 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4681 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4741 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     4801 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4861 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4921 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4981 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5041 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     5101 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     5161 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     5221 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     5281 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5341 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5401 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5461 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5761 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6181 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6541 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6601 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     6661 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     6721 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     6781 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     6841 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     6901 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     6961 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7021 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7081 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7141 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7261 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7321 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7381 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7441 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7561 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7621 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7921 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8161 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9481 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9541 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     9601 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     9661 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     9721 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9781 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9841 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9901 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9961 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    10021 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    10081 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    10141 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    10201 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    10261 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
    10321 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10381 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10441 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10501 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10561 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10621 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    10681 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    10741 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10801 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10861 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10921 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10981 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    11041 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    11101 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    11161 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    11221 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11281 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    11341 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    11401 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    11461 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    11521 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    11581 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    11641 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    11701 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    11761 gtttcagacc cacctcccaa ccccgagggg ac
//
