LOCUS       sequence-24443-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: AF0837(128-241)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             403..420
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(473..491)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      487..534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(657..676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcgacattc cctctagaat attttgttta ctttaagaag gagatataca tatgcgcccc
       61 gaaatcgtgc tgctgaccat tgaggaaggt tacgctgttg caggagttct gaggcagtgg
      121 ggtgttgagg agatttttga ggagaggatg ggctacggaa agggtatggg ggatagcaga
      181 aaagagttct tcggagaggt tgcggcaaag cttgagagct tcgattttaa atacctcatc
      241 gttgctggac cgggattcgc caaaaacgat tttctcgact ttttgaagga gaggtatcct
      301 gagatggcta agaatgccgt tgtggtggac gtttcctccg tcggctcaag gggcttcatt
      361 gaaattttga agagaagggt cgttgacaaa atagttctcg agcaccacca ccaccaccac
      421 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      481 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      541 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttangcgcgg
      601 cgggtgtggn ggttacgcgc agcctgaccg ctaccttgcc agcgccctac cgcccgctcc
      661 tttcgctttc ttcccttccn ttcncgccac gttcgccggc tttcccgcan nacgnctncg
      721 gggccncgcc ttcagggttc cgattagtgc ttacgcactc gacccaaaaa cttgatangg
      781 tgatgntcac gtatggcatc gcctgaaacg tttcgccttg cttgatcngt cttaantgan
      841 ctgtcaatgg nnacnacctt ccgnatctga taaga
//
