LOCUS       sequence-244431-       12217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NOTCH2NLB full length under CAG promoter 
            with mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, 
            Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen 
            P
  TITLE     Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through 
            Delta/Notch Regulation.
  JOURNAL   Cell. 2018 May 31;173(6):1370-1384.e16. doi: 
            10.1016/j.cell.2018.03.067. Epub 2018 May 31.
  PUBMED    29856955
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.03.067"; journalName: "Cell"; date: 
            "2018-05-31- 31"; volume: "173"; issue: "6"; pages: "1370-1384.e16"
COMMENT     Sequence Label: pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry
FEATURES             Location/Qualifiers
     source          1..12217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3185..4201
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4125..4147
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4209..4228
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4264..4283
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4264..4282
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4297..4315
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4297..4314
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5173..5190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5215..5244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5254..5283
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5293..5322
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5332..5361
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5371..5400
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5434..5463
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(5518..6092,6093..6095,6096..6104)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5685..5702)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5912..5931
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6136..6846
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6281..6299)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6549..6568
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6906..7494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6959..6979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7377..7388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7497..7513)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8022..8202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8228..8245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8234..8458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8504..8932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8591..8610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8801..8822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8941..8961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8946..9275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9126..9261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9188..9207
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9323..9370
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9389..9763
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9893..10014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9930..9949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9984..10003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10063..10079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10063..10079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10076..10098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10087..10103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10111..10117,10118..10135,10136..10141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10156..10177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10294..10311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10465..11053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10545..10564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11224..12015,12016..12084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11847..11866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12085..12189)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsggggg
     3061 gsggggsggg gsgrggsggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa
     3121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3181 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3241 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3301 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3361 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3421 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3481 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3541 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3601 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3661 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3721 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3781 ggcggggccg ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc
     3841 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     3901 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     3961 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     4021 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     4081 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4141 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     4201 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctcga
     4261 gcgtaatacg actcactata gggagaccca agcttgattt aggtgacact atagaataca
     4321 agctacttgt tctttttgca gaccaccatg cccgccctgc gccccgctct gctgtgggcg
     4381 ctgctggcgc tctggctgtg ctgcgcgacc cccgcgcatg cattgcagtg tcgagatggc
     4441 tatgaaccct gtgtaaatga aggaatgtgt gttacctacc acaatggcac aggatactgc
     4501 aaatgtccag aaggcttctt gggggaatat tgtcaacatc gagacccctg tgagaagaac
     4561 cgctgccaga atggtgggac ttgtgtggcc caggccatgc tggggaaagc cacgtgccga
     4621 tgtgcctcag ggtttacagg agaggactgc cagtactcga catctcatcc atgctttgtg
     4681 tctcgacctt gcctgaatgg cggcacatgc catatgctca gccgggatac ctatgagtgc
     4741 acctgtcaag tcgggtttac aggtaaggag tgccaatgga ccgatgcctg cctgtctcat
     4801 ccctgtgcaa atggaagtac ctgtaccact gtggccaacc agttctcctg caaatgcctc
     4861 acaggcttca cagggcagaa gtgtgagact gatgtcaatg agtgtgacat tccaggacac
     4921 tgccagcatg gtggcatctg cctcaacctg cctggttcct accagtgcca gtgccttcag
     4981 ggcttcacag gccagtactg tgacagcctg tatgtgccct gtgcaccctc gccttgtgtc
     5041 aatggaggca cctgtcggca gactggtgac ttcacttttg agtgcaactg ccttccagaa
     5101 acagtgagaa gaggaacaga gctctgggaa agagacaggg aagtctggaa tggaaaagaa
     5161 cacgatgaga atcaccacca tcatcaccac ggatcccatc gatttaaagc tatggagcaa
     5221 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
     5281 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
     5341 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
     5401 aatgaaatgg agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac
     5461 ttgaatttag agatctagaa ttagatgtgt gttacctacg ctagcgttaa cgatccgccc
     5521 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     5581 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     5641 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     5701 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     5761 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     5821 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     5881 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     5941 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac
     6001 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6061 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccggctgg agacgtggag
     6121 gagaaccctg gacctatggt gagcaagggc gaggaggata acatggccat catcaaggag
     6181 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6241 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     6301 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     6361 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     6421 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     6481 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6541 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6601 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6661 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6721 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6781 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6841 aagtaagcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta
     6901 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7501 accgtcgacc tcgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7561 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7621 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7681 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7741 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7801 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7861 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7921 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7981 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8161 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8221 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8401 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8461 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     8521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     8941 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9001 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9181 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9301 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9361 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     9421 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     9481 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     9541 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     9601 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     9661 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     9721 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     9781 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9841 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     9901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10021 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12181 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
