LOCUS       sequence-244436-        2844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector to clone and measure the expression of promoter 
            candidates (spike-in) by determining the abundance of transcripts 
            originating from the candidate in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pSTAP-seq_human_spike-in
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    393..514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     484..503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(636..655)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          638..770
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    896..987
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(928..947)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    1001..1049
                     /note="synthetic polyadenylation signal"
     CDS             join(1154..1222,1223..2014)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2214..2802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2703..2722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      121 gtgagcgagg aagcggaaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
      181 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
      241 gttgaaggct ctcagggccc ctctcaaagc ttgagggcat cggtcggatc cttatcgatt
      301 ttaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      361 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgctcgaag cggccggccg
      541 aattctctag atcaatctaa ttcaacaaga attgggacaa ctccagtgaa gagttcttct
      601 cctttgctag ccatgcttaa gcctgcaggg tggacacctg tggagagaaa ggcaaagtgg
      661 atgtcagtaa gaccaatagg tgcctatcag aaacgcaaga gtcttctctg tctcgacaag
      721 cccagtttct attggtctcc ttaaacctgt cttgtaacct tgatacttac ctgcccagtg
      781 cctcacgacc gggccatggc cgaattcgtc gacgaaatcg atatcaccgg tgcatgctct
      841 aagatctcga gcccgggcta gcacgcgtaa gagctcggta ccggccagtt aggccagaga
      901 aatgttctgg cacctgcact tgcactgggg acagcctatt ttgctagttt gttttgtttc
      961 gttttgtttt gatggagagc gtatgttagt actatcgatt cacacaaaaa accaacacac
     1021 agatgtaatg aaaataaaga tattttattg cggcctgtcc aatacctccc gtaccttaat
     1081 attacttact tatccttgag agacgtacta gtaaccctga taaatgcttc aataatattg
     1141 aaaaaggacg agtatgagca tccaacattt tcgtgtcgca ctcattccct tctttgcggc
     1201 attttgcttg cctgtttttg cacaccccga aacgctggtg aaagtaaaag atgctgaaga
     1261 tcaactgggt gcaagagtgg gctatatcga actggatctc aatagcggca agatccttga
     1321 gtctttccgc cccgaagaac gattcccgat gatgagcact ttcaaagtac tgctatgtgg
     1381 cgcggtgttg tcccgtatag acgccgggca agagcagctt ggtcgccgta tacactactc
     1441 acaaaacgac ttggttgagt actcgccggt cacggaaaag catcttacgg atggcatgac
     1501 ggtaagagaa ttgtgtagtg ctgccattac catgagcgac aacaccgcgg ccaacttact
     1561 tctgacaacg atcggaggcc ctaaggagct gactgcattt cttcataata tgggtgatca
     1621 tgtgacccgg cttgaccgct gggaaccaga gttgaacgaa gccataccga acgacgagcg
     1681 tgataccacg atgccagtag caatggccac aactcttcgg aaactactca ctggcgaact
     1741 tcttactcta gcatcacgac agcagctcat agactggatg gaggcggaca aagtagcagg
     1801 accacttctt cgctcggccc tccctgctgg ctggttcatt gctgacaaat cgggggccgg
     1861 tgaacgcggc tctcgcggca tcattgctgc gctggggcct gatggtaagc cctcacgaat
     1921 cgtagtgatc tacacgacgg ggagtcaggc cactatggac gaacgaaata ggcagatcgc
     1981 tgagatcggt gcctcactga tcaagcactg gtaaccactg cagtggttta gcatttgcgg
     2041 ccgctgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2821 tttttacggt tcctggcctt ttgc
//
