LOCUS       sequence-244438-        5122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAP-seq luciferase validation vector, which harbors an 4x UAS array
            upstream of the CP position (candidates are inserted at CP position)
            for the recruitement of GAL4-DBD tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pGL3_4xUAS_CP-candidate_luc+
FEATURES             Location/Qualifiers
     source          1..5122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    13..134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(24..43)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     78..97
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(382..399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(553..1141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(633..652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1312..2103,2104..2172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1935..1954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2173..2277)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2304..2759
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2391..2410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2601..2622
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2890..2938
                     /note="synthetic polyadenylation signal"
     misc_feature    2952..3043
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2992..3011
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    3084..3108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3201..3225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3436..3445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3442..5094
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3508..3526)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
       61 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      121 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      181 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
      241 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      301 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      361 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      961 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1621 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1921 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2281 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2341 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2401 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2461 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2521 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2581 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2641 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2701 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     2761 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2821 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg
     2881 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     2941 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     3001 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata ggtacctgag
     3061 ctcgctagcc tcgaggatat caaacaagtt tgtacaaaaa agcaggcttc gtcggagtac
     3121 tgtcctccga gcggagtact gtcctccgag gccgccgccc ggagtactgt cctccgagcg
     3181 gagtactgtc ctccgaggtg acccagcttt cttgtacaaa gtggtatatc aagatctgcg
     3241 atcgagctcg cccggggatc gagcgcagcg gtataaaagg gcgcggggtg gctgagagca
     3301 tcagttgtga atgaatgttc gagccgagca gacgtgccgc tgccttcgtt aatatccttt
     3361 gaataagcca actttgaatc acaagacgca taccaaaccc tgcaggaagc ttggcattcc
     3421 ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg cccggcgcca
     3481 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     3541 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac
     3601 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     3661 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     3721 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     3781 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg
     3841 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat
     3901 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     3961 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca
     4021 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga
     4081 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     4141 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     4201 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     4261 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc
     4321 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     4381 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg
     4441 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc
     4501 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     4561 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga
     4621 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     4681 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     4741 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa
     4801 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc
     4861 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     4921 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     4981 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     5041 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag
     5101 agtcggggcg gccggccgct tc
//
