LOCUS       sequence-244463-        6516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pENTR_hEMSY
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(13..99)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     148..170
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     162..179
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     163..179
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    195..294
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             576..587
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      3370..3379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4267..4366)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4383..4402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4384..4402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4407..4423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4407..4423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4525..4544)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4536..5345
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4603..4622)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5492..6080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5981..6000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6234..6251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6410..6437)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
       61 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
      121 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
      181 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      241 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggcttcatgc
      301 ctgttgtgtg gccaaccctt ctggatctca gcagggatga atgcaaaaga attcttcgaa
      361 aattggaatt ggaggcatat gctggagtta tcagtgcact tcgggcacag ggggatctca
      421 ccaaggaaaa gaaagatctt cttggagaac tatcaaaagt tcttagcatc tcaacagaac
      481 gccaccgtgc tgaagttcgg agagcagtaa acgatgaacg gttaacaaca attgcacata
      541 atatgtctgg acctaatagc tcttcagaat ggtccattga aggtcgtcga ttggtaccac
      601 tgatgccccg gctcgttccc caaaccgcct ttactgtaac agctaatgct gttgctaatg
      661 cagctatcca gcataatgca tctcttccag tgcctgcaga aacaggaagc aaggaagtag
      721 tggtttgcta ttcctacaca agtaccacgt caaccccaac ctctacccct gttccaagtg
      781 gcagcatagc aacggttaag tctccaagac ctgccagtcc tgcctccaat gtagttgtct
      841 tgccaagtgg aagtactgtt tatgtcaaaa gtgtaagctg ttcagatgaa gatgaaaaac
      901 ccagaaaacg aaggcgaaca aactcttcca gctcctctcc tgttgttcta aaggaagttc
      961 caaaggccgt tgttccagtc tcaaagacga tcactgtgcc tgtgagtggt agtcccaaga
     1021 tgagcaacat catgcagagc attgccaact ccttaccacc ccacatgtct cctgtaaaaa
     1081 taaccttcac taaaccatca acacagacaa caaacacaac aacacagaag gttattatag
     1141 tcaccacatc accaagctca accttcgtgc ccaacattct ctccaaatcc cataactatg
     1201 cagcagtcac taagcttgta ccaacgtcag tcattgcttc tacaacccag aagccaccag
     1261 ttgttataac tgcttcacag tcctctctgg tcagtaatag cagcagtggc agcagcagtt
     1321 ctacaccatc acctattcct aatacagttg cagtaacagc tgtggtgtcc tctacaccat
     1381 ctgtggtcat gtcaacagta gcacaaggtg tatctacatc agcaatcaaa atggcatcaa
     1441 ccagacttcc ttcccccaaa agcttagtga gtgccccaac tcagattctt gcacagttcc
     1501 ctaaacaaca tcaacagtct cctaagcagc agttatatca agtgcaacag cagacacagc
     1561 aacaagtggc ccagccttct ccagtatctc atcagcaaca gcctcagcag tctcctttgc
     1621 cacctggtat taaacctacc atccaaatca aacaggagtc aggtgttaaa atcatcacac
     1681 aacaggttca accaagtaaa atcttaccca aaccagtgac agcaactcta cccaccagta
     1741 gcaattcccc tattatggtg gttagcagta atggtgcaat tatgacaact aaactggtaa
     1801 ccactcctac tggcacacaa gcaacctata cccggccaac agtgagccca tccattggtc
     1861 ggatggctgc aacccctgga gctgcaacct atgtgaaaac tacgagtggt agcatcatta
     1921 cagtagtacc caaatcatta gctaccttgg ggggcaagat aattagcagt aatatagttt
     1981 ctggaacgac taccaaaatc actacaatcc caatgacttc caagcccaac gtgattgttg
     2041 tacaaaagac tacaggaaaa ggaacgacca ttcaaggcct cccgggcaaa aatgttgtca
     2101 caacgttgct aaatgctgga ggagaaaaga ctattcagac agtgccaaca ggagcaaagc
     2161 cagctatcct tactgctaca agacccatca ccaaaatgat tgtaacgcag ccaaaaggaa
     2221 taggttctac agttcaacca gcagctaaaa tcatcccaac aaaaattgtt tatgggcagc
     2281 aagggaaaac gcaggttctt attaaaccca aaccagtgac ttttcaagcg acagttgtta
     2341 gtgaacaaac aagacagcta gtaacagaaa cattacagca agcatccagg gtagcagagg
     2401 ctggtaattc atctattcag gaaggaaaag aagaaccaca gaattataca gatagtagtt
     2461 cctcttctac agagtcctcc cagagttccc aagattccca gcctgtagtt catgtaattg
     2521 cttcccggcg tcaggattgg tcagaacatg agattgcaat ggagactagc cctaccataa
     2581 tttatcagga tgtatccagt gaatcacaat cagctacttc aacaatcaaa gctctgttag
     2641 aactccaaca gacaacagta aaggaaaaat tggaatctaa accaagacaa cccactattg
     2701 acctgagtca aatggcagtg cctattcaga tgacccagga aaagagacat tctcctgaga
     2761 gtccatcaat tgctgtggta gagtcagaac tagtagctga atacatcact actgaacgca
     2821 ctgatgaggg gacagaggtt gcttttcccc ttctagtcag ccatcgctcc cagccccaac
     2881 agccttccca gccccagcgg accctgctcc agcatgtggc tcagtcacag accgcaacac
     2941 agacttcggt ggtggtgaag tccatcccag catcttcccc tggagcaatc acccacatta
     3001 tgcagcaggc attaagcagt cacactgctt ttaccaaaca cagcgaggaa cttggaactg
     3061 aggagggcga ggttgaagag atggacactt tagaccctca gacaggtctg ttttaccgat
     3121 ctgccctgac tcagtcacag tcagctaaac agcagaaact tagccagccc ccgctggaac
     3181 agactcagct gcaagtgaaa actctgcagt gcttccagac taaacagaag cagaccatcc
     3241 acctgcaggc agaccagctc cagcacaaac tcccgcaaat gccccagctt tccatcaggc
     3301 atcaaaaact cacccctctc cagcaagaac aagcacagcc caagccagat gtacagcaca
     3361 cacagcatcc catggtggcc aaagacaggc agcttcctac cttaatggca cagcccccgc
     3421 aaactgtagt acaggtgctt gcagtgaaaa ccacgcagca gctccctaaa ctgcagcagg
     3481 ctccgaacca accaaaaatc tacgtgcaac cccaaacccc ccagagccaa atgtcgctcc
     3541 cagcttcttc agagaaacag acggcaagcc aggtggagca gccaattata acccaaggat
     3601 cctctgttac aaagataact tttgaggggc gccagcctcc cacagttaca aagataactg
     3661 gtggcagttc tgtgcctaag ctgacatcac cagttacaag catatctccc attcaggcct
     3721 ctgagaagac agcagtgtct gacattttga aaatgtcttt gatggaagct cagattgata
     3781 caaatgtaga acatatgata gtggatcccc caaagaaggc tcttgccact agcatgctca
     3841 ctggtgaagc aggatcatta ccctccaccc acatggtggt ggcagggatg gcgaattcca
     3901 ctccccagca acagaaatgt agagagtcct gttcgagtcc atccaccgtt ggctcttccc
     3961 taacgacaag gaaaattgat ccaccagcag tgcctgcgac aggccagttc atgcgtattc
     4021 agaatgtagg ccaaaagaaa gctgaagaga gtccagcaga aattatcatc caggctattc
     4081 ctcagtatgc tattccttgt cactccagct ccaatgtggt ggtggagccc agtgggcttc
     4141 ttgagctaaa caacttcact agtcaacagc tggatgatga ggagacagca atggagcagg
     4201 acatagacag tagcacggag gatggaactg aacccagccc ttctcagagc tctgctgaac
     4261 ggtccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     4321 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     4381 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     4441 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     4501 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     4561 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     4621 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     4681 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     4741 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     4801 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     4861 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     4921 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     4981 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     5041 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     5101 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     5161 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     5221 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     5281 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     5341 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     5401 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     5461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6121 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6181 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6241 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6301 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6361 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     6421 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     6481 ccaccctccg ggccgttgct tcacaacgtt caaatc
//
