LOCUS       sequence-244464-        7020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged Myosin-19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Lopez-Domenech G, Covill-Cooke C, Ivankovic D, Halff EF, Sheehan DF,
            Norkett R, Birsa N, Kittler JT
  TITLE     Miro proteins coordinate microtubule- and actin-dependent 
            mitochondrial transport and distribution.
  JOURNAL   EMBO J. 2018 Feb 1;37(3):321-336. doi: 10.15252/embj.201696380. Epub
            2018 Jan 8.
  PUBMED    29311115
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201696380"; journalName: "EMBO J"; date: "2018-02-1- 
            1"; volume: "37"; issue: "3"; pages: "321-336"
COMMENT     Sequence Label: GFP-Myo19
FEATURES             Location/Qualifiers
     source          1..7020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3808..3929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3845..3864)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3899..3918
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3936..4391)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4073..4094)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4285..4304
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4418..4522
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4524..4881
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4524..4715
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4547..4567)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4732..4867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4916..5710
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4916..5710
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4970..4989)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5580..5599
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5898..5917)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5942..5989
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6318..6906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6807..6826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatgctc cagcaggtca atggccacaa tccggggtct
     1381 gatggccaag ccagggagta cctcagagaa gacctgcagg agttcctggg tggggaggtc
     1441 ctgctgtaca aactggatga cctcaccagg gtgaatcctg tgacactaga gacagtcctg
     1501 aggtgcctgc aggcccggta catggcagac acattctaca ccaatgctgg ctgcaccctg
     1561 gtagccttga accccttcaa gcctgttcct cagctctact cgcccgagct aatgagagag
     1621 taccatgctg cgcctcagcc ccagaaactg aagccccatg tgttcactgt gggtgaacag
     1681 acctacagga atgtcaagag cctgattgaa ccagtcaacc agtctattgt tgtcagtgga
     1741 gagagtggtg ctggaaagac atggacgtct cgctgcctaa tgaagttcta tgctgtggtg
     1801 gccacctcac ctgcatcttg ggagagccac aagattgcag agaggataga acagaggatc
     1861 ctgaactcca accctgtcat ggaagctttt gggaatgcgt gtacactgag gaataacaac
     1921 agcagtcgct ttgggaagtt catccagctc cagctgaaca gggctcagca aatgactgga
     1981 gccgcagtcc agacctacct cctagagaaa actcgagtgg cctgccaggc ttccagtgag
     2041 aggaacttcc acatcttcta tcagatttgc aaaggagcca gtgaggacga gaggctccag
     2101 tggcaccttc ctgagggagc tgccttctcc tggctgccca acccagagag gagcttagaa
     2161 gaggattgtt ttgaggtgac cagagaggcc atgctccatt tgggcattga cacccctacc
     2221 cagaacaaca tctttaaggt cctagctgga ctgctgcacc ttggcaatat ccagtttgct
     2281 gcctccgagg atgaagccca gccctgccag ccgatggatg atgccaagta ctctgtcagg
     2341 acggcagcct cgctgctggg gctcccagag gacgtgctgc tggagatggt gcagattaga
     2401 accatcaggg caggcagaca gcagcaggtg ttccggaagc cctgcgcccg agccgagtgt
     2461 gacacccgta gagactgcct ggccaaactg atctatgcgc ggttgtttga ctggctggta
     2521 tcagtgatca acagcagcat ctgtgcagac accgactcgt ggaccacttt cataggcctg
     2581 ctggatgtgt atggatttga atcatttcct gacaacagtc tggaacagtt gtgcatcaac
     2641 tacgccaatg agaagctgca gctggaggcc tgtggcctcg tggagaccat ccatatcagt
     2701 gctgctggct tccccatccg ggtctctcac cgaaactttg tagaacgata caagttacta
     2761 agaaggcttc atccttgcac atcctctggc cccgacagcc catatcctgc caaagggctc
     2821 cctgaatggt gtccacacag cgaggaagcc acgcttgaac ctctcatcca ggacattctc
     2881 cacactctgc cggtcctaac tcaggcagca gccataactg gtgactcggc tgaggccatg
     2941 ccagccccca tgcactgtgg caggaccaag gtgttcatga ctgactctat gctggagctt
     3001 ctggaatgtg ggcgtgcccg ggtgctggag cagtgtgccc gctgcatcca gggtggctgg
     3061 aggcgacacc ggcaccgaga gcaggagcgg cagtggcggg ccgtcatgct catccaggca
     3121 gccattcgtt cctggttaac tcggaaacac atccagaggc tgcatgcagc tgccacagtc
     3181 atcaagcgtg catggcagaa gtggagaatc agaatggcct gccttgctgc taaagagctg
     3241 gatggtgtgg aagaaaaaca cttctctcaa gctccctgtt ccctgagcac ctcgccgctg
     3301 cagaccaggc tcctggaggc aataatccgc ctctggcccc tgggactggt cctggccaat
     3361 acggctatgg gtgtaggcag ctttcagagg aaattagtgg tctgggcttg cctccagctc
     3421 cccaggggca gccccagtag ctacactgtc cagacagcac aagaccaggc tggtgtcacg
     3481 tccatccgag cgctgcctca gggatcgata aagtttcact gcagaaagtc tccactgcgg
     3541 tatgctgaca tctgccctga accttcaccc tacagcatta caggctttaa tcagattctg
     3601 ctggaaagac acaggctgat ccacgtgacc tcttctgcct tcactgggct ggggtgagtc
     3661 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     3721 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     3781 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     3841 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3901 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     3961 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4021 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4081 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4141 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4201 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4261 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4321 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4381 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4441 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4501 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     4561 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4621 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4681 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4741 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4801 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4861 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     4921 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4981 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5041 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     5101 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5161 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5221 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5281 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5341 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5401 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     5461 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5521 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5581 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5641 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5701 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5761 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5821 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5881 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     5941 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     6001 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     6061 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     6121 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
