LOCUS       sequence-244466-        6520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Miro1 with the transmembrane deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Lopez-Domenech G, Covill-Cooke C, Ivankovic D, Halff EF, Sheehan DF,
            Norkett R, Birsa N, Kittler JT
  TITLE     Miro proteins coordinate microtubule- and actin-dependent 
            mitochondrial transport and distribution.
  JOURNAL   EMBO J. 2018 Feb 1;37(3):321-336. doi: 10.15252/embj.201696380. Epub
            2018 Jan 8.
  PUBMED    29311115
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201696380"; journalName: "EMBO J"; date: "2018-02-1- 
            1"; volume: "37"; issue: "3"; pages: "321-336"
COMMENT     Sequence Label: myc-Miro1 (lacking TM)
FEATURES             Location/Qualifiers
     source          1..6520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2765..2774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2778..2912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2815..2834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2869..2888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2985..3342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2985..3176
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3008..3028)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3193..3328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3362..3379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3362..3378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3591..4046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3678..3697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3888..3909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4063..4082)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4217..4239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4292..4310)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4378..4482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4483..4551,4552..5343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4701..4720)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5514..6102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6003..6022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6256..6273
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(6426..6431,6432..6449,6450..6456)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6464..6480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6469..6491
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6488..6504
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6488..6504
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tagggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaaggcg gattccccgt
      721 gccaagagtg acgtaagtac cgcgtataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attggccacc atggagcaga agctgatctc cgaggaggac
      961 ctgggatcta tgaagaaaga cgtgcggatc ctgctggtgg gagaacctag agttgggaag
     1021 acatcactga ttatgtctct ggtcagtgaa gaatttccag aagaggttcc tccccgggca
     1081 gaagaaatca ccattccagc tgatgtcacc ccagagagag ttccaacaca cattgtagat
     1141 tactcagaag cagaacagag tgatgaacaa cttcatcaag aaatatctca ggctaatgtc
     1201 atctgtatag tgtatgccgt taacaacaag cattctattg ataaggtaac aagtcgatgg
     1261 attcctctca taaatgaaag aacagacaaa gacagcaggc tgcctttaat attggttggg
     1321 aacaaatctg atctggtgga atatagtagt atggagacca tccttcctat tatgaaccag
     1381 tatacagaaa tagaaacctg tgtggagtgt tcagcgaaaa acctgaagaa catatcagag
     1441 ctcttttatt acgcacagaa agctgttctt catcctacag ggcccctgta ctgcccagag
     1501 gagaaggaga tgaaaccagc ttgtataaaa gcccttactc gtatatttaa aatatctgat
     1561 caagataatg atggtactct caatgatgct gaactcaact tctttcagag gatttgtttc
     1621 aacactccat tagctcctca agctctggag gatgtcaaga atgtagtcag aaaacatata
     1681 agtgatggtg tggctgacag tgggttgacc ctgaaaggtt ttctcttttt acacacactt
     1741 tttatccaga gagggagaca cgaaactact tggactgtgc ttcgacgatt tggttatgat
     1801 gatgacctgg atttgacacc tgaatatttg ttccccctgc tgaaaatacc tcctgattgc
     1861 actactgaat taaatcatca tgcatattta tttctccaaa gcacctttga caagcatgat
     1921 ttggatagag actgtgcttt gtcacctgat gagcttaaag atttatttaa agttttccct
     1981 tacatacctt gggggccaga tgtgaataac acagtttgta ccaatgaaag aggctggata
     2041 acctaccagg gattcctttc ccagtggacg ctcacgactt atttagatgt acagcggtgc
     2101 ctggaatatt tgggctatct aggctattca atattgactg agcaagagtc tcaagcttca
     2161 gctgttacag tgacaagaga taaaaagata gacctgcaga aaaaacaaac tcaaagaaat
     2221 gtgttcagat gtaatgtaat tggagtgaaa aactgtggga aaagtggagt tcttcaggct
     2281 cttcttggaa gaaacttaat gaggcagaag aaaattcgtg aagatcataa atcctactat
     2341 gcgattaaca ctgtttatgt atatggacaa gagaaatact tgttgttgca tgatatctca
     2401 gaatcggaat ttctaactga agctgaaatc atttgtgatg ttgtatgcct ggtatatgat
     2461 gtcagcaatc ccaaatcctt tgaatactgt gccaggattt ttaagcaaca ctttatggac
     2521 agcagaatac cttgcttaat cgtagctgca aagtcagacc tgcatgaagt taaacaagaa
     2581 tacagtattt cacctactga tttctgcagg aaacacaaaa tgcctccacc acaagccttc
     2641 acttgcaata ctgctgatgc ccccagtaag gatatctttg ttaaattgac aacaatggcc
     2701 atgtatccgc acgtgacaca agctgacctc aagagctcca cgttttgatt cctgcagaag
     2761 cttggccgcc atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2881 aactcatcaa tgtatcttat catgtctgga tcgatcggga attaattcgg cgcagcacca
     2941 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     3001 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3061 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3241 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt aacagcttgg
     3361 cactggccgt cgttttacaa cgtggtgact gggaaaaccc tggcgttacc caacttaatc
     3421 gccttgcagc acatcccccc ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3481 gcccttccca acagttgcgt agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     3541 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttgcgattta gtgctttacg
     3781 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3901 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3961 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4021 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4081 cgcatagtta agccaactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     4141 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4201 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4261 cgcgcgaggc agtattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4321 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgatga cgccgggcaa
     4741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgccagcagc aatggcaaca
     5041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5641 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5881 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     5941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6181 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6241 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6301 ccgcctctcc ccgcgcgttg gccgattcat taatccagct ggcacgacag gtttcccgac
     6361 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     6421 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6481 tttcacacag gaaacagcta tgaccatgat tacgaattaa
//
