LOCUS       sequence-244474-        6072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Zhao B, Ye X, Yu J, Li L, Li W, Li S, Yu J, Lin JD, Wang CY, 
            Chinnaiyan AM, Lai ZC, Guan KL
  TITLE     TEAD mediates YAP-dependent gene induction and growth control.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1962-71.
  PUBMED    18579750
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1962-71."
COMMENT     Sequence Label: pCMX-Gal4-TEAD1
FEATURES             Location/Qualifiers
     source          1..6072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        269..648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        649..852
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     803..823
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     897..916
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        897..915
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             948..1388
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          2758..2823
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2953..2973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3245..3379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3282..3301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3336..3355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3837..3971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4036..4053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4207..4795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4287..4306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4966..5757,5758..5826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5589..5608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5827..5931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5999..6017
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(6055..6072,1..5))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
       61 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      121 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
      181 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      241 ggcctcttcg ctattacgcc agcgcgttga cattgattat tgactagtta ttaatagtaa
      301 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      361 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      421 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      481 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      541 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      601 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      661 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      721 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      781 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      841 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      901 acgactcact atagggagac ccaagcttga agcaagcctc ctgaaagatg aagctactgt
      961 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa
     1021 aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca
     1081 aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg
     1141 aacagctatt tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt
     1201 ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag
     1261 atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc
     1321 atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga
     1381 ctgtatcgcc ggaattcgaa aggatgagtg actctgcaga taagccaatt gacaatgatg
     1441 cagaaggggt ctggagcccc gacatcgagc aaagctttca ggaggccctg gctatctatc
     1501 caccatgtgg gaggaggaaa atcatcttat cagacgaagg caaaatgtat ggtaggaatg
     1561 aattgatagc cagatacatc aaactcagga caggcaagac gaggaccaga aaacaggtgt
     1621 ctagtcacat tcaggttctt gccagaagga aatctcgtga ttttcattcc aagctaaagg
     1681 atcagactgc aaaggataag gccctgcagc acatggcggc catgtcctca gcccagatcg
     1741 tctcggccac tgccattcat aacaagctgg ggctgcctgg gattccacgc ccgaccttcc
     1801 caggggcgcc ggggttctgg ccgggaatga ttcaaacagg gcagccagga tcctcacaag
     1861 acgtcaagcc ttttgtgcag caggcctacc ccatccagcc agcggtcaca gcccccattc
     1921 cagggtttga gcctgcatcg gccccagctc cctcagtccc tgcctggcaa ggtcgctcca
     1981 ttggcacaac caagcttcgc ctggtggaat tttcagcttt tctcgagcag cagcgagacc
     2041 cagactcgta caacaaacac ctcttcgtgc acattgggca tgccaaccat tcttacagtg
     2101 acccattgct tgaatcagtg gacattcgtc agatttatga caaatttcct gaaaagaaag
     2161 gtggcttaaa ggaactgttt ggaaagggcc ctcaaaatgc cttcttcctc gtaaaattct
     2221 gggctgattt aaactgcaat attcaagatg atgctggggc tttttatggt gtaaccagtc
     2281 agtacgagag ttctgaaaat atgacagtca cctgttccac caaagtttgc tcctttggga
     2341 agcaagtagt agaaaaagta gagacggagt atgcaaggtt tgagaatggc cgatttgtat
     2401 accgaataaa ccgctcccca atgtgtgaat atatgatcaa cttcatccac aagctcaaac
     2461 acttaccaga gaaatatatg atgaacagtg ttttggaaaa cttcacaatt ttattggtgg
     2521 taacaaacag ggatacacaa gaaactctac tctgcatggc ctgtgtgttt gaagtttcaa
     2581 atagtgaaca cggagcacaa catcatattt acaggcttgt aaaggactga gtcgacggta
     2641 ccagatatca ggatcctggc cagctagcta ggtagctaga ggatctttgt gaaggaacct
     2701 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     2761 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     2821 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     2881 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     2941 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     3001 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     3061 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg
     3121 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3181 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3241 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3301 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3361 atcttatcat gtctggatca gcttcagaag atggcggagg gcctccaaca cagtaatttt
     3421 cctcccgact cttaaaatag aaaatgtcaa gtcagttaag caggaagtga ctaactgacc
     3481 gcagctggcc gtgcgacatc ctcttttaat tagttgctag gcaactgccc tccagagggc
     3541 agtgtggttt tgcaagagga agcaaaaagc ctctccaccc aggcctagaa tgtttccacc
     3601 caatcattac tatgacaaca gctgtttttt ttagtattaa gcagaggccg ggggcccctg
     3661 gcctccgctt actctggaga aaaagaagag aggcattgta gaggcttcca gaggcaactt
     3721 gtcaaaacag gactgcttct atttctgtca cactgtctgg ccctgtcaca aggtccagca
     3781 cctccatacc ccctttaata agcagtttgg gaacgggtgc gggtcttact ccgcccatcc
     3841 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3901 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3961 tttttggagg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4861 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4921 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4981 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5041 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5101 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5161 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5221 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5281 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5341 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5401 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5461 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5521 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5581 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5641 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5701 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5761 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5821 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5881 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5941 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6001 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     6061 acacatgcag ct
//
