LOCUS       sequence-244475-        3237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone encoding human MAP1LC3C wild-type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Agrotis A, Pengo N, Burden JJ, Ketteler R
  TITLE     Redundancy of human ATG4 protease isoforms in autophagy and 
            LC3/GABARAP processing revealed in cells.
  JOURNAL   Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.
  PUBMED    30661429
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2019 Jan 21. doi: 10.1080/15548627.2019.1569925."
COMMENT     Sequence Label: pDONR223 LC3C WT
FEATURES             Location/Qualifiers
     source          1..3237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3..21)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26..42)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             473..1264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1357..1945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1846..1865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2099..2116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2275..2302)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2394..2480)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2529..2551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2543..2560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2544..2560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2576..2675
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3123..3222)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
       61 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
      121 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
      181 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
      241 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      301 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
      361 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
      421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
      481 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      541 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      601 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      661 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      721 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
      781 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      841 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      901 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      961 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1021 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1081 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1141 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1201 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1261 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     1321 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1381 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1441 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1501 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1561 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1621 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1681 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1741 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1801 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1861 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1921 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1981 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2041 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2101 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2161 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2221 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     2281 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     2341 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     2401 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     2461 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     2521 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
     2581 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
     2641 ttttataatg ccaactttgt acaaaaaagc aggcttaatg ccgcctccac agaaaatccc
     2701 aagcgtcaga cccttcaagc agaggaaaag cttggcaatc agacaagagg aagttgctgg
     2761 aatccgggca aagttcccca acaaaatccc ggtggtagtg gagcgctacc ccagggagac
     2821 gttcctgccc ccgctggaca aaaccaagtt cctggtcccg caggagctga ccatgaccca
     2881 gttcctcagc atcatccgga gccgcatggt cctgagagcc acggaagcct tttacttgct
     2941 ggtgaacaac aagagcctgg tcagcatgag cgcaaccatg gcagagatct acagagacta
     3001 caaggatgag gatggcttcg tgtacatgac ctacgcctcc caggagacat ttggctgcct
     3061 ggagtcagca gcccccaggg atgggagcag ccttgaggac agaccctgca atcctctcta
     3121 atacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3181 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatat
//
