LOCUS       sequence-24451-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: AF0837(242-344)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             369..386
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(439..457)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      453..500
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgcgtcattc cctctgaata ttttgtttac tttaagaagg agatatacat atgggggaag
       61 ttcgtcttgc tgaggaggcg gagtatatag acaggctgct tgaagggata gcgaagggag
      121 agagagtcgc ttacggtctg gatgaggtca gggaggcgca caattacagg gcgattgagg
      181 ttttgctcgt tgcggatgag tttctgcttg aggagaggga gaagtgggat gttgacggac
      241 tgctcaggga agttgaggag tcgggcggaa aggtggtgat aatgagcacc gaattcgagc
      301 cgggtaagag gctgatgagc ctcggtggaa tagctgccct cctgagattc aacgtaaaag
      361 gtctcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      421 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      481 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      541 gccctgtagc ggcgcattaa gcgcggcgtn cgtttnttta acccncagtt angaccgcta
      601 cacttgcang cctacgccct cttcgtcctt tcgctttctt cccttccttt ctcgccacgt
      661 tcgccggctt tccccgtcaa gctctaatcg gggctccctt taggttccga ttagtgctta
      721 cggcactcga ccccaaaaac ttgatanggt gtggttccgt antggcatcg cctganaang
      781 ntttcgcctt gacntgatcn ngtcttatat gatctgtcaa tgaaanctca cctttngcnt
      841 tcttgt
//
