LOCUS       sequence-244514-        9846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for cell size.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Berenson DF, Zatulovskiy E, Xie S, Skotheim JM
  TITLE     Constitutive expression of a fluorescent protein reports the size of
            live human cells.
  JOURNAL   Mol Biol Cell. 2019 Nov 15;30(24):2985-2995. doi: 
            10.1091/mbc.E19-03-0171. Epub 2019 Oct 10.
  PUBMED    31599704
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E19-03-0171"; journalName: "Mol Biol Cell"; date: 
            "2019-11-15- 15"; volume: "30"; issue: "24"; pages: "2985-2995"
COMMENT     Sequence Label: CSII-prEF1a-E2-Crimson-NLS
FEATURES             Location/Qualifiers
     source          1..9846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3774..4448
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     4172..4191
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4452..4472
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4541..5129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4594..4614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5012..5023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5648..5828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5854..5871)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5860..6084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6130..6558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6217..6236)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6427..6448
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6567..6587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6572..6901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6752..6887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6814..6833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6949..6996
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7015..7389
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7519..7640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7556..7575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7610..7629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7689..7705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7689..7705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7702..7724)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7713..7729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7737..7743,7744..7761,7762..7767))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7782..7803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7920..7937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8091..8679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8171..8190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8850..9641,9642..9710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9473..9492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9711..9815)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttca gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ctcgagaatt cggatggata
     3781 gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag ggctccgtga
     3841 acggccacga gttcgagatc gagggcgtgg gcgagggcaa gccctacgag ggcacccaga
     3901 ccgccaagct gcaagtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     3961 cccagttctt ctacggctcc aaggcgtaca tcaagcaccc cgccgacatc cccgactacc
     4021 tcaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4081 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccctcatc taccacgtga
     4141 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actctgggct
     4201 gggagccctc cactgagcgc aactaccccc gcgacggcgt gctgaagggc gagaaccaca
     4261 tggcgctgaa gctgaagggc ggcggccact acctgtgtga gttcaagtcc atctacatgg
     4321 ccaagaagcc cgtgaagctg cccggctacc actacgtgga ctacaagctc gacatcacct
     4381 cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc cgccaccacc
     4441 tgttccagcc tccaaaaaag aagagaaagg tagaagaccc cggggatcca ccggtcgcca
     4501 cctagtctag agttaacatc gagggatcaa gcttatcgat aatcaacctc tggattacaa
     4561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4921 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     4981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5041 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5101 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgagacctag aaaaacatgg
     5161 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     5221 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     5281 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5341 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     5401 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     5461 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     5521 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     5581 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     5641 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5821 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5881 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5941 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6001 tggggggtgg gttggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6061 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6121 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6541 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6901 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6961 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     7021 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     7081 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7141 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7201 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     7261 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     7321 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     7381 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7441 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7501 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7621 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7681 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7741 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7801 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7981 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8041 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8521 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9841 gacgtc
//
