LOCUS       sequence-244520-        4443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pENTR_Trf2
FEATURES             Location/Qualifiers
     source          1..4443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1982..2081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2098..2117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2099..2117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2122..2138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2240..2259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2251..3060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2318..2337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3207..3795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3696..3715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3949..3966
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4125..4152)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4244..4330)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4379..4401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4393..4410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4394..4410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4426..4443,1..82)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtaca aaaaagcagg cttccaaaac gatatggtca gcataccggt ggccaatttg
      121 aacggcggcc ttaaagcggc cagcagcggt tccggagttg gtgtagtgac ctccggcggc
      181 gtcgtatcct cggcggtgct ggccaatgcc ccacgtgtat acctaacgcc ctcctccaca
      241 ttcatgacca atcgccaaat ggcgggagtg gcgtccaccg gaagaatgag cggacaagtc
      301 gtcggtggtt catctggcac agcctcaaca gccggtaccg ttcgctattt ttcgcaattc
      361 agcaaaatgc aaacggccgg cggacccagt ttgcaaagga aacttgccaa cggggatacc
      421 attgttctgg caaccggcag taaaaatatg ttccttacta gcagcgaaaa taaggctaat
      481 ctgccgaccg tcgccagcaa tggcaatggt ctgatcacag ccaaaatgga tctattggag
      541 gaggaggtca tgcagtcgat aactgtgatt gatgacgatg acgaggagaa aaaggaagtc
      601 gctgaagacg aagaagagag cagcaataat gccaaaccca ttgaccttca ccagccaatt
      661 gctgataatg agcacgagct ggacattgtg ataaacaatg tggtctgctc gtttagcgtc
      721 ggctgtcacc tgaagctgcg cgaaattgcg ctgcagggat caaatgtgga gtaccgtcgc
      781 gagaacggaa tggtgacaat gaagctccgc catccataca ccactgcctc gatttggtcg
      841 tcggggagga ttacctgcac tggtgccact tccgagtcaa tggccaaggt cgctgcacgc
      901 cgctatgcca gatgccttgg caagctggga tttcccactc gtttcctcaa ctttcgcatt
      961 gtcaacgtac tgggcacctg cagcatgccg tgggccatca agatcgtcaa cttttcggag
     1021 cgccatcgcg agaacgccag ctacgagccc gagctgcatc ctggagtgac ttacaagatg
     1081 cgcgatcccg atccaaaggc caccctgaag atcttctcca ccggcagcgt aacagtcacc
     1141 gcggctagcg taaaccacgt tgaatcggct atccagcaca tatacccgct ggtctttgat
     1201 ttccgcaagc aacgttcggc ggaggagctg cagcatctgc gtcaaaagca gcgtctgcaa
     1261 gccggcggtg atccccacga actggagaaa aatgtcttgg ccgataacaa gactgcttcc
     1321 cttgataata tctttgttaa tacaacagcg gcgcactcta aatcatcgtc gaatgaccaa
     1381 acatcagcac ccgcgaccat cttgtccagc acagttgata gtatgcctcg tctgaaacag
     1441 atggtcaact atcaccagat gatgaagcaa acccaggaag agcgccgcca tataatgttt
     1501 aatggggaga aggccaatcc ggcctccacc tcatcggcgg cagcagctcc ctccacctcc
     1561 tcctcatcgt cctcatccgg ggataatatc tgtgccaatg cccgtcgccg ggccaccgaa
     1621 tgctgggcaa ccaagctgca gaataagcgt ccacgttaca atgatcccgg taccacgggc
     1681 acaattaacg ccgcatcgtc cacagcatca gccgccacat cttcgttagc ctcccaggcg
     1741 acacatctgc gtaatcctct caagacggcc gcgttggcca atgcacgtat gttaggcgcc
     1801 aaggtgacaa catgcacacg caacagcata atcgtgcagc agccgcaacg tatccaaatg
     1861 cagcagcagc agcaacagct gcaaccgcag cagcaacaga ccagcttctc gcccagcgaa
     1921 ttcgatgttg atgacttgat cgaggaggag gagaacaacg aattggatat gcccttctag
     1981 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2041 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2101 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2161 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2221 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2281 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2341 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2401 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2461 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2521 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2581 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2641 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2701 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2761 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2821 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2881 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2941 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3001 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3061 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3121 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3841 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3901 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3961 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4021 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4081 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4141 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4201 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4261 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4321 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4381 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
     4441 ttt
//
