LOCUS       sequence-244524-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone encoding human GABARAPL2 G116A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Agrotis A, Pengo N, Burden JJ, Ketteler R
  TITLE     Redundancy of human ATG4 protease isoforms in autophagy and 
            LC3/GABARAP processing revealed in cells.
  JOURNAL   Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.
  PUBMED    30661429
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2019 Jan 21. doi: 10.1080/15548627.2019.1569925."
COMMENT     Sequence Label: pDONR223 GABARAPL2 G116A
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             155..946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1039..1627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1528..1547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1781..1798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1957..1984)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2076..2162)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2211..2233
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2225..2242
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2226..2242
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2258..2357
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2715..2814)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2831..2850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2832..2850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2855..2871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2855..2871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
       61 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
      121 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
      181 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
      241 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
      301 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
      361 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
      421 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
      481 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
      541 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
      601 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
      661 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
      721 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
      781 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
      841 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
      901 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
      961 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     1021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1141 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1321 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1381 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1441 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1501 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1561 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1621 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1681 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1741 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1801 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1861 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     1921 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     1981 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     2041 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     2101 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     2161 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     2221 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     2281 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     2341 gtacaaaaaa gcaggcttaa tgaagtggat gttcaaggag gaccactcgc tggaacacag
     2401 atgcgtggag tccgcgaaga ttcgagcgaa atatcccgac agggttccgg tgattgtgga
     2461 aaaggtctca ggctctcaga ttgttgacat tgacaaacgg aagtacttgg ttccatctga
     2521 tatcactgtg gctcagttca tgtggatcat caggaaaagg atccagcttc cttctgaaaa
     2581 ggcgatcttc ctgtttgtgg ataagacagt cccacagtcc agcctaacta tgggacagct
     2641 ttacgagaag gaaaaagatg aagatggatt cttatatgtg gcctacagcg gagagaacac
     2701 ttttgccttc taatacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2761 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2821 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2881 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     2941 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     3001 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     3061 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     3121 cagcggtggt aacggcgcag tggcggt
//
