LOCUS       sequence-244542-        2657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Slot A, empty (step 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Santos-Moreno J, Schaerli Y
  TITLE     A Framework for the Modular and Combinatorial Assembly of Synthetic 
            Gene Circuits.
  JOURNAL   ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 
            10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
  PUBMED    31185158
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00174"; journalName: "ACS Synth Biol"; date: 
            "2019-07-19- 19"; volume: "8"; issue: "7"; pages: "1691-1697"
COMMENT     Sequence Label: pTU-A-013
FEATURES             Location/Qualifiers
     source          1..2657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      654..740
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      832..859
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(988..1779)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDDGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1780..1871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1919..2657)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgctc ctaggatgca gtgactcgca catttccccg aaaagtgcca
      181 cctgaattca taggatagat tctggaaact ttaccgtccg agctcaggct taccttactc
      241 gagcaataaa cagttgatag ggcttctccg ttaccatggt tcagccaaaa aacttaagac
      301 cgccggtctt gtccactacc ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc
      361 tcttctcaat tcttctgacc tgtaacgaat aatagatagt aaagtagtct ccgattgagt
      421 tttctctgcc gagtcccacc cagttctgtg atttcagtaa gttggtaatt gatacactgt
      481 tgcgagaact gctgcctggt agtagatagg ttgttattga gtaagaaggt aaagtgaacg
      541 aaatccctga aactgagact gtagaaaata agctttgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgccagg catcaaataa
      661 aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      721 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg
      781 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca
      841 tcctgacgga tggccttttt gcgtttcaga tctaccggta aaccagcaat agacataagc
      901 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc
      961 ccctcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
     1021 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     1081 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1141 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1201 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1261 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1321 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1381 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1441 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1501 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1561 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     1621 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg tcatccactg cggagccgta
     1681 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     1741 tagttgagtc gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta
     1801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1861 aaataaacaa atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc
     1921 gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg
     1981 aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga
     2041 gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa
     2101 tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg
     2161 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
     2221 tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
     2281 aggtcgtttg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
     2341 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
     2401 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
     2461 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
     2521 cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
     2581 ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc
     2641 aagaagatcc tttgatc
//
