LOCUS       sequence-244598-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Tan BS, Yang MC, Singh S, Chou YC, Chen HY, Wang MY, Wang YC, Chen 
            RH
  TITLE     LncRNA NORAD is repressed by the YAP pathway and suppresses lung and
            breast cancer metastasis by sequestering S100P.
  JOURNAL   Oncogene. 2019 Jul;38(28):5612-5626. doi: 10.1038/s41388-019-0812-8.
            Epub 2019 Apr 9.
  PUBMED    30967631
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0812-8"; journalName: "Oncogene"; date: 
            "2019-07"; volume: "38"; issue: "28"; pages: "5612-5626"
COMMENT     Sequence Label: pLAS3W-S100P
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    114..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        408..711
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          989..2005
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1929..1951
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2013..2032
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2059..2077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2420..2993,2994..2996,2997..3005)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2586..2603)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2813..2832
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3006..3800
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3060..3079)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3670..3689
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3810..4398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3863..3883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4281..4292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4491..4724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4796..4917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4833..4852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4887..4906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4957..5092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5113..5131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5141..5158)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5141..5157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5150..5172)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5299..5754
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5386..5405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5596..5617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5780..5884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5885..5953,5954..6745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6103..6122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6916..7504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7405..7424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7658..7675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7792..7813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7828..7833,7834..7851,7852..7858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7866..7882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7871..7893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7890..7906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7890..7906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7925..7945
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7927..7945
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7973..8199
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8200..8380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8427..8552
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9045..9278
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9463..9507
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagttttaaa
      121 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      181 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tactagtgaa
      241 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      301 aaattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttctag ttattaatag
      361 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      421 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      481 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      541 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      601 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      661 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      721 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      781 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggsgcgcgcc
      841 rggsggggsg gggsggggsg rggggggggg sggggsgrgg sggrgaggkg cggcggcagc
      901 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      961 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1021 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1081 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1141 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1201 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1261 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1321 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1381 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1441 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1501 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     1561 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggrgg gctcgggggr
     1621 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1681 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     1741 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     1801 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1861 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     1921 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1981 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2041 tcattttggc aaagaattta atacgactca ctatagggag acccaagctg gctagcatga
     2101 cggaactaga gacagccatg ggcatgatca tagacgtctt ttcccgatat tcgggcagcg
     2161 agggcagcac gcagaccctg accaaggggg agctcaaggt gctgatggag aaggagctac
     2221 caggcttcct gcagagtgga aaagacaagg atgccgtgga taaattgctc aaggacctgg
     2281 acgccaatgg agatgcccag gtggacttca gtgagttcat cgtgttcgtg gctgcaatca
     2341 cgtctgcctg tcacaagtac tttgagaagg caggactcaa atgagaattc ctgcagggtt
     2401 taaactgtgt taagatccgc ccctctccct ccccccccct aacgttactg gccgaagccg
     2461 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2521 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2581 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2641 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2701 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     2761 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     2821 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     2881 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     2941 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3001 caaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3061 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3121 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3181 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3241 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3301 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3361 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3421 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     3481 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     3541 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     3601 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     3661 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     3721 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     3781 ttcttgacga gttcttctga agggtcgaca atcaacctct ggattacaaa atttgtgaaa
     3841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3961 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4021 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4081 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4141 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4201 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4261 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4321 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4381 tttgggccgc ctccccgcgg atccacgcgt gactctagat gtacctttaa gaccaatgac
     4441 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4501 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     4741 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     4801 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4861 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4921 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4981 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5041 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     5101 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     5161 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5221 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5281 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5341 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5401 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5461 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5521 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5581 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5641 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5701 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5761 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5821 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5881 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5941 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6001 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6061 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7681 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7921 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     7981 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     8041 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     8101 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     8161 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     8221 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8281 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8341 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8401 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8461 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8521 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8581 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8641 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8701 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8761 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8821 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8881 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     8941 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9001 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9061 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9121 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9181 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9241 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9301 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9361 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9421 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9481 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9541 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     9601 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9661 ctcccaaccc cgag
//
