LOCUS       sequence-244600-        6139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Tan BS, Yang MC, Singh S, Chou YC, Chen HY, Wang MY, Wang YC, Chen 
            RH
  TITLE     LncRNA NORAD is repressed by the YAP pathway and suppresses lung and
            breast cancer metastasis by sequestering S100P.
  JOURNAL   Oncogene. 2019 Jul;38(28):5612-5626. doi: 10.1038/s41388-019-0812-8.
            Epub 2019 Apr 9.
  PUBMED    30967631
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0812-8"; journalName: "Oncogene"; date: 
            "2019-07"; volume: "38"; issue: "28"; pages: "5612-5626"
COMMENT     Sequence Label: RK5F-YAP8SA
FEATURES             Location/Qualifiers
     source          1..6139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2404..2427
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2439..2448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2452..2586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2489..2508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2543..2562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2655..3012
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2655..2846
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2678..2698)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2863..2998
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3032..3049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3032..3048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3041..3063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3261..3716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3348..3367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3558..3579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3733..3752)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3852..3874
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3912..3930)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3998..4102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4103..4171,4172..4963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4321..4340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5134..5722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5623..5642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5876..5893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6010..6031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6046..6051,6052..6069,6070..6076)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6084..6100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6089..6111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6108..6124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6108..6124
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcatggat cccgggcagc agccgccgcc tcaaccggcc
      961 ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc gccgtccgga
     1021 cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc cgccgggcat
     1081 cagatcgtgc acgtccgcgg ggacgcggag accgacctgg aggcgctctt caacgccgtc
     1141 atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg gaagctgccc
     1201 gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccgc tactgatgca
     1261 ggcactgcag gagccctgac tccacagcat gttcgagctc atgccgctcc agctgctctg
     1321 cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc tggcccagca
     1381 gctacaccca cagctcagca tcttcgacag gctgcttttg agatacctga tgatgtacct
     1441 ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt cttaaatcac
     1501 atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca gatgaacgtc
     1561 acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc aggtcctctt
     1621 cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat aaaccataag
     1681 aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat gaaccagaga
     1741 atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag cccacaggga
     1801 ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca gcaactgcag
     1861 atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcagga gttagccctg
     1921 cgtagccagt taccaacact ggagcaggat ggtgggactc aaaatccagt gtcttctccc
     1981 gggatgtctc aggaattgag aacaatgacg accaatagct cagatccttt ccttaacagt
     2041 ggcacctatc actctcgaga tgaggctaca gacagtggac taagcatgag cagctacagt
     2101 gtccctcgaa ccccagatga cttcctgaac agtgtggatg agatggatac aggtgatact
     2161 atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc cagactacct tgaagccatt
     2221 cctgggacaa atgtggacct tggaacactg gaaggagatg gaatgaacat agaaggagag
     2281 gagctgatgc caagtctgca ggaagctttg agttctgaca tccttaatga catggagtct
     2341 gttttggctg ccaccaagct agataaagaa agctttctta catggttatc tagagtcgac
     2401 atggactaca aggacgacga cgacaagtga taagcttggc cgccatggcc caacttgttt
     2461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2581 tggatcggga attaattcgg cgcagcacca tggcctgaaa taacctctga aagaggaact
     2641 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2761 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2821 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2881 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2941 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3001 ggcttttgca aaaagctgtt aacagcttgg cactggccgt cgttttacaa cgtcgtgact
     3061 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3121 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3181 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3241 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3301 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3361 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3421 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     3481 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3541 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3601 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3661 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3721 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3781 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3841 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3901 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3961 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4021 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4081 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4141 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4201 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4261 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4321 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4381 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4441 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4501 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4561 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4621 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4681 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4741 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4801 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4861 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4921 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4981 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5041 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5101 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5161 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5221 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5281 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5341 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5401 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5461 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5521 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5581 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5641 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5701 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5761 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5821 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5881 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5941 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6001 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6061 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6121 tgacatgatt acgaattaa
//
