LOCUS       sequence-244602-       10066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A
            and an antibiotic resistance cassette (zeocin). Used for selection 
            of endothelial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10066)
  TITLE     CDH5-GFP-AB
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CDH5-AbR-GFP
FEATURES             Location/Qualifiers
     source          1..10066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2361..2381
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3878..3902
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3923..4588
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             3923..4588
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             4730..4783
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(5184..5208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5261..5302
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5326..5914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5379..5399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5797..5808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5933..6432
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5968..5991)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6365..6384
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6446..6493
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6512..6910
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6997..7230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7302..7423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7339..7358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7393..7412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7463..7598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7618..7637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7619..7637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7647..7664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7647..7663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7656..7678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7805..8260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7892..7911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8102..8123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8286..8390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8391..8459,8460..9251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8609..8628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9422..10010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9911..9930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatgtc
     2341 gacgttaacg ctagtgatat caactttgta tagaaaagtt ggctccgaat tcgcccttca
     2401 aatgtcagag ggtccaagtc ctccctccct ccaagctcat ccatgcccat ggcctcagat
     2461 gccagccata agctgttggg ttccaaacct cgactccagg ctggactcac ccctgtctcc
     2521 cccaccagcc tgacacctcc acctgggtat ctaacgagca tctcaaactc aacctgcctg
     2581 agacagagga atcactatcc cctcctcctc caaaaatatc cttccatcac actccccatc
     2641 ttgtgctctg atttactaaa cggccctggg ccctctcttt ctcagggtct ctgcttgccc
     2701 agctatataa taaaacaagt ttgggacttc ccaaccattc acccatggaa aaacagaagc
     2761 aactcttcaa aggacagatt cccaggatct gccctgggag attccaaatc agttgatctg
     2821 gggtgagccc agtcctctgt agtttttaga agctcctcct atgtctctcc tggtcagcag
     2881 aatcttggcc cctcccttcc ccccagcctc ttggttcttc tgggctctga tccagcctca
     2941 gcgtcactgt cttccacgcc cctctttgat tctcgtttat gtcaaaagcc ttgtgaggat
     3001 gaggctgtga ttatccccat tttacagatg aggaaactgt ggctccagga tgacacaact
     3061 ggccagaggt cacatcagaa gcagagctgg gtcacttgac tccacccaat atccctaaat
     3121 gcaaacatcc cctacagacc gaggctggca ccttagagct ggagtccatg cccgctctga
     3181 ccaggagaag ccaacctggt cctccagagc caagagcttc tgtccctttc ccatctcctg
     3241 aagcctccct gtcaccttta aagtccattc ccacaaagac atcatgggat caccacagaa
     3301 aatcaagctc tggggctagg ctgaccccag ctagattttt ggctctttta taccccagct
     3361 gggtggacaa gcaccttaaa cccgctgagc ctcagcttcc cgggctataa aatgggggtg
     3421 atgacacctg cctgtagcat tccaaggagg gttaaatgtg atgctgcagc caagggtccc
     3481 cacagccagg ctctttgcag gtgctgggtt cagagtccca gagctgaggc cgggagtagg
     3541 ggttcaagtg gggtgcccca ggcagggtcc agtgccagcc ctctgtggag acagccatcc
     3601 ggggccgagg cagccgccca ccgcagggcc tgcctatctg cagccagccc agccctcaca
     3661 aaggaacaat aacaggaaac catcccaggg ggaagtgggc cagggccagc tggaaaacct
     3721 gaaggggagg cagccaggcc tccctcgcca gcggggtgtg gctcccctcc aaagacggtc
     3781 ggctgacagg ctccacagag ctccactcac gctcagccct ggacggacag gcagtccaac
     3841 ggaacagaaa catccctcag ccaagggcga attcgaccca agtttgtaca aaaaagcagg
     3901 ctccgcggcc gcccccttca ccatgcccgc catgaagatc gagtgccgca tcaccggcac
     3961 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcacccccg agcagggccg
     4021 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     4081 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     4141 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     4201 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     4261 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     4321 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     4381 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     4441 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     4501 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     4561 cgccttcaag acccccatcg ccttcgccag atctcgagat atcagccatg gcttcccgcc
     4621 ggcggtggcg gcgcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat
     4681 ggaccgtcac cctgcagcct gtgcttctgc taggatcaat gtgaccggtg agggcagagg
     4741 aagtcttcta acatgcggtg acgtggagga gaatcccggc ccttccggta tggccaaact
     4801 cacttctgca gtcccagtcc tcacagcaag ggatgttgca ggggctgtag agttctggac
     4861 tgacagatta ggattctcca gagactttgt tgaagatgat tttgctggtg ttgtcagaga
     4921 tgatgtcacc ctcttcatct cagcagttca ggaccaagtt gtccctgaca acacccttgc
     4981 ttgggtctgg gtcagaggcc tagatgagct ttatgcagaa tggtcagaag tagtcagcac
     5041 aaatttcagg gatgcctctg gcccagccat gacagaaatt ggtgaacaac cttggggaag
     5101 ggaatttgcc ctcagagacc ctgctggaaa ttgtgtccat tttgtagctg aggaacagga
     5161 ctagtcgaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatccagca
     5221 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     5281 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga tcgacaatca acctctggat
     5341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5701 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5881 acgagtcgga tctccctttg ggccgcctcc ccgcctggcg atggtaccta ccgggtaggg
     5941 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     6001 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     6061 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     6121 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     6181 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     6241 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     6301 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     6361 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     6421 ccgggccttt cgactctaga cacgtgttga caattaatca tcggcatagt atatcggcat
     6481 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc
     6541 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     6601 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     6661 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     6721 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     6781 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     6841 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     6901 ggagggctaa gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     6961 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7021 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7081 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7141 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7201 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7261 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7441 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7561 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7621 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7681 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7741 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7801 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7861 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7921 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7981 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8041 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8101 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8161 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8221 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8281 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8341 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8401 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8461 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8521 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8581 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8641 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8701 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8761 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8821 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8881 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8941 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9001 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9061 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9541 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctca
//
