LOCUS       sequence-244603-       10651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral or direct expression of cytosolic jRGECO1a in 
            excitatory neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou
            S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
  TITLE     Wide-area all-optical neurophysiology in acute brain slices.
  JOURNAL   J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019.
  PUBMED    30952812
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019."
COMMENT     Sequence Label: SLF060: Lenti-CaMKIIa-jRGECO1a
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1768..3056
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3138..3155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3159..3191
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3195..3203,3204..3218)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             3246..3302
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     3998..4017
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4509..5097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4562..4582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4980..4991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5100..5116)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5101..5117)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5622..5802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5828..5845)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5834..6058
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6104..6532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6191..6210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6401..6422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6541..6561)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6546..6875
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6726..6861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6788..6807
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6923..6970
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6989..7363
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7493..7614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7530..7549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7584..7603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7663..7679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7663..7679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7676..7698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7687..7703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7711..7717,7718..7735,7736..7741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7756..7777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7894..7911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8065..8653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8145..8164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8824..9615,9616..9684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9447..9466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9685..9789)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9864..9883)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10055..10434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10436..10634
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10589..10609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaacat tatggcctta ggtcacttca tctccatggg
     1801 gttcttcttc tgattttcta gaaaatgaga tgggggtgca gagagcttcc tcagtgacct
     1861 gcccagggtc acatcagaaa tgtcagagct agaacttgaa ctcagattac taatcttaaa
     1921 ttccatgcct tgggggcatg caagtacgat atacagaagg agtgaactca ttagggcaga
     1981 tgaccaatga gtttaggaaa gaagagtcca gggcagggta catctacacc acccgcccag
     2041 ccctgggtga gtccagccac gttcacctca ttatagttgc ctctctccag tcctaccttg
     2101 acgggaagca caagcagaaa ctgggacagg agccccagga gaccaaatct tcatggtccc
     2161 tctgggagga tgggtgggga gagctgtggc agaggcctca ggaggggccc tgctgctcag
     2221 tggtgacaga taggggtgag aaagcagaca gagtcattcc gtcagcattc tgggtctgtt
     2281 tggtacttct tctcacgcta aggtggcggt gtgatatgca caatggctaa aaagcaggga
     2341 gagctggaaa gaaacaagga cagagacaga ggccaagtca accagaccaa ttcccagagg
     2401 aagcaaagaa accattacag agactacaag ggggaaggga aggagagatg aattagcttc
     2461 ccctgtaaac cttagaaccc agctgttgcc agggcaacgg ggcaatacct gtctcttcag
     2521 aggagatgaa gttgccaggg taactacatc ctgtctttct caaggaccat cccagaatgt
     2581 ggcacccact agccgttacc atagcaactg cctctttgcc ccacttaatc ccatcccgtc
     2641 tgttaaaagg gccctatagt tggaggtggg ggaggtagga agagcgatga tcacttgtgg
     2701 actaagtttg ttcgcatccc cttctccaac cccctcagta catcaccctg ggggaacagg
     2761 gtccacttgc tcctgggccc acacagtcct gcagtattgt gtatataagg ccagggcaaa
     2821 gaggagcagg ttttaaagtg aaaggcaggc aggtgttggg gaggcagtta ccggggcaac
     2881 gggaacaggg cgtttcggag gtggttgcca tggggacctg gatgctgacg aaggctcgcg
     2941 aggctgtgag cagccacagt gccctgctca gaagccccaa gctcgtcagt caagccggtt
     3001 ctccgtttgc actcaggagc acgggcaggc gagtggcccc tagttctggg ggcagcgggg
     3061 gatccgccac catgctgcag aacgagcttg ctcttaagtt ggctggactt gatattaaca
     3121 agactggagg aggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
     3181 agcaaatggg tcgggatctg tacgacgatg acgataagga tctcgcaaca atggtcgact
     3241 catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt cggctgagct
     3301 cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga
     3361 aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg
     3421 ccaagaagcc cgtgcagctg cccggcgcct acatcgtgga catcaagttg gacatcgtgt
     3481 cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc cgccactcca
     3541 ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg agcaagggcg
     3601 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     3661 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct
     3721 ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     3781 tgtcccctca gttcatgtac ggctccaagg cctacattaa gcacccagcc gacatccccg
     3841 actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg
     3901 acggcggcat tattcacgtg aaccaggact cctccctgca ggacggcgta ttcatctaca
     3961 aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     4021 tgggctggga ggctacgcgt gacgacctga ctgaagagca gatcgcagaa tttaaagagg
     4081 ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag cttgggacgg
     4141 tgttccgatc gctggggcag aaccccacag aagcagagct gcaggacatg atcaatgaag
     4201 tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg atggcaagaa
     4261 aaatgaatga cacagacagt gaagaggaaa ttcgcgaagc gttccgcgtg tttgataagg
     4321 acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac cttggagaga
     4381 agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat ggggatggtc
     4441 aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtaggaattc gatatcaagc
     4501 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4561 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4621 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4681 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4741 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4801 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4861 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4921 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4981 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5041 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5101 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     5161 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     5221 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5281 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     5341 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     5401 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5461 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     5521 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     5581 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     5641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5761 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     5821 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5881 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5941 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6001 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6061 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6541 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6781 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6901 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     6961 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     7021 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     7081 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     7141 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     7201 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     7261 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     7321 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     7381 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7441 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7621 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9781 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9841 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9901 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     9961 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
    10021 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
    10081 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
    10141 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    10201 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
    10261 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
    10321 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
    10381 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
    10441 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
    10501 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
    10561 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
    10621 gtctatataa gcagcgcgtt ttgcctgtac t
//
