LOCUS       sequence-244614-       10878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral or direct expression of eTsChR in excitatory neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou
            S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
  TITLE     Wide-area all-optical neurophysiology in acute brain slices.
  JOURNAL   J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019.
  PUBMED    30952812
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019."
COMMENT     Sequence Label: SLF091: Lenti-CaMKIIa-eTsChR-eGFP
FEATURES             Location/Qualifiers
     source          1..10878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2365..3653
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             join(4614..4616,4617..5330)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4614..4616,4617..5327)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4656..4677)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4917..4936)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5264..5285
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5355..5943
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5408..5428)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5826..5837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5946..5962)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5947..5963)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6468..6648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6674..6691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6680..6904
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6950..7378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7037..7056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7247..7268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7387..7407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7552..7685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7612..7631
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7747..7794
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7813..8187
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8317..8438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8354..8373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8408..8427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8487..8503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8487..8503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8500..8522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8511..8527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8535..8541,8542..8559,8560..8565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8580..8601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8718..8735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8889..9477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8969..8988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9648..10439,10440..10508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10271..10290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10509..10613)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10688..10707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct ccatggggtt
     2401 cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca gtgacctgcc
     2461 cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa tcttaaattc
     2521 catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta gggcagatga
     2581 ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc cgcccagccc
     2641 tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc taccttgacg
     2701 ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca tggtccctct
     2761 gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg
     2821 tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg gtctgtttgg
     2881 tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa gcagggagag
     2941 ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc ccagaggaag
     3001 caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat tagcttcccc
     3061 tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc tcttcagagg
     3121 agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc agaatgtggc
     3181 acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca tcccgtctgt
     3241 taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca cttgtggact
     3301 aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg gaacagggtc
     3361 cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca gggcaaagag
     3421 gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg gggcaacggg
     3481 aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag gctcgcgagg
     3541 ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa gccggttctc
     3601 cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc agcgggggat
     3661 ccaccatgtt cgccatcaac cccgaatata tgaatgagac tgtgctgctg gacgaatgca
     3721 ccccaatcta cctggatatt ggaccactgt gggagcaggt ggtggctagg gtgactcagt
     3781 ggttcggagt gatcctgtct ctggtgtttc tgatctacta catctggaac acttacaagg
     3841 ctacctgtgg gtgggaggaa ctgtacgtgt gcacagtgga gttctgtaag atcatcatcg
     3901 aactgtactt cgagtatact ccccctgcca tgatcttcca gacaaatgga caagtgactc
     3961 cctggctgcg gtatgctgag tggctgctga catgccctgt gatcctgatt cacctgtcta
     4021 acattactgg gctgaatgac gattacagtg gccgcaccat gagcctgatc acatccgacc
     4081 tgggcggaat ttgtatggct gtgaccgccg ctctgagcaa gggatggctg aaagccctgt
     4141 tctttgtgat cgggtgcggc tacggagcca gcaccttcta caacgccgct tgtatctaca
     4201 ttgagtccta ctataccatg ccacagggaa tttgcaggag actggtgctg tggatggccg
     4261 gggtgttctt tacatcctgg ttcatgtttc ctggcctgtt cctggctgga ccagaaggaa
     4321 cccaggccct gtcttgggct ggaaccacaa tcggacatac agtggccgac ctgctgagta
     4381 agaacgcttg gggaatgatc gggcactttc tgagggtgga gatccacaag catatcatca
     4441 tccatggaga tgtgcggcgc cccgtgacag tgaaggctct ggggaggcag gtgagcgtga
     4501 attgtttcgt ggacaaagag gaagaggaag aggatgagag aatcgcggcc gccaagagca
     4561 ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac gtggtgagca
     4621 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4681 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4741 ccctgaagtt catttgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4801 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4861 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4921 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4981 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5041 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5101 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5161 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5221 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5281 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag gaattcgata
     5341 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5401 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5461 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5521 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5581 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5641 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5701 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5761 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5821 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5881 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5941 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6001 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6061 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6121 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6181 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     6241 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6301 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     6361 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     6421 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     6481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     6661 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6721 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6781 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6841 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6901 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7381 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7441 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7501 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7561 taactccgcc catcccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7621 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7681 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7741 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7801 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     7861 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     7921 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     7981 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     8041 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     8101 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     8161 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     8221 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8281 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8341 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8401 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8461 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8521 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8581 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8641 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8701 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8761 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8821 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8881 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8941 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9001 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9061 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9301 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9541 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9601 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9661 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9721 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9781 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9841 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9901 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9961 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10021 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10081 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10141 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10201 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10261 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10321 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10381 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10441 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10501 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10561 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10621 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    10681 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    10741 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    10801 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    10861 gggccagata tacgcgtt
//
