LOCUS       sequence-244617-       11315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express p65-Clover under p65 promoter (With 
            puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Lane K, Van Valen D, DeFelice MM, Macklin DN, Kudo T, Jaimovich A, 
            Carr A, Meyer T, Pe'er D, Boutet SC, Covert MW
  TITLE     Measuring Signaling and RNA-Seq in the Same Cell Links Gene 
            Expression to Dynamic Patterns of NF-kappaB Activation.
  JOURNAL   Cell Syst. 2017 Apr 26;4(4):458-469.e5. doi: 
            10.1016/j.cels.2017.03.010. Epub 2017 Apr 5.
  PUBMED    28396000
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2017.03.010"; journalName: "Cell Syst"; date: 
            "2017-04-26- 26"; volume: "4"; issue: "4"; pages: "458-469.e5"
COMMENT     Sequence Label: pLenti_Puro_DEST mouse_p65promoter_mouse_p65-C...
FEATURES             Location/Qualifiers
     source          1..11315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1952..1976
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2005..2057
                     /label=MCS
                     /note="multiple cloning site"
     CDS             4613..5155
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     primer_bind     complement(5255..5276)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5863..5884
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5920..5944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5975..6563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6028..6048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6446..6457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6577..7076
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6612..6635)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7009..7028
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7097..7696
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7097..7116)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7593..7613
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7834..8067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8139..8260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8176..8195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8230..8249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8300..8435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8455..8474)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8456..8474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8484..8501)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8484..8500)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8493..8515)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8642..9097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8729..8748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8939..8960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9123..9227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9228..9296,9297..10088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9446..9465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10259..10847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10748..10767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11001..11018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11135..11156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11171..11176,11177..11194,11195..11201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11209..11225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11214..11236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11233..11249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11233..11249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11268..11288
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11270..11288
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa
     1981 aggaaccaat tcagtcgact ggatctcgag ctcaagcttc gaattctgca gtcgacggta
     2041 ccgcgggccc gggatccacc ggccggtcgc caccatgatt atcgactctt aactgatggg
     2101 ccatctctcc agcctgcaac catccttttt taaagagcat cttctgtagt tctggatggg
     2161 ttcaaactat gtagaggagg atgacgttga acgtctgacc ccatgagcgc tgggattact
     2221 ggagcatcct ggagtcagag atcaaatccc agcttcctgc aacgttatgc aaaaactact
     2281 gagttctcat ttctagcctt tactttttag taaggacgct ggtattcata ttaacttaaa
     2341 ttgtaactat atgtgttttt acaagtggtt catgtgagga ccaatgatgg ctgtgtgcca
     2401 ttaattggag attatattca ttctgtcgcc ttgacatccg ccctcgcaaa atattaaact
     2461 gctttttcta atctgcgcca agtgagaaac tgaaatgagt gggaggggcg taactatttt
     2521 cacttttgtc ttcctgtttt tttcagagcc tgggaaagca attcagaatt tccaatcttt
     2581 tccaattccc acatgatttc ctaatttaga gaaaataagt ctttctctga gtgctgaaac
     2641 ctcctgtggc ccgggaggcg cctcctcctt ctggcatagt ggaggcagcc tgatgtgttg
     2701 cgtcctccgg ccagagtccc cacactcaat ctgcacacct gcccttggct tagatggcaa
     2761 tttttgctcc ctacaaacgc tttcctcatt ggagcctgga ctcctgggag gccttgaagg
     2821 agatgttcac ggtgtgaccc tagctggttc cggagatccc taaagatcta aaccttagca
     2881 gacagcacct atgtgtgcag acgaagccct ggggctcttc cagcactcat ggctctcagg
     2941 gactcttaga ggtacaggtg tctgcctagt cctcggtctg cccctctacc ctaaggcctg
     3001 tcggatgcag cacgctcgct ccgcgaagca ctcctgcgcg cagccggatc taggttgggg
     3061 agcatgtggc ccgtagcacg cgggtcccca gaaggcccct gcacgcttag gaaaacgtgt
     3121 ggaattcagt cactggcctc cgcgggagca gcaggcctgg gcctctccct gcgcagggcg
     3181 aatggccgca gcctgccacc agccctggct cggctccagt cccggccagg gtgacatcac
     3241 caaactcctg agatcagagc ggggcgggga acgggacccg aaatccccta aaaacagagt
     3301 gagtaatcgc gggcccgccc tccaggcggg gccgggaacg ggaccgggac cccgagcgag
     3361 ccgcgctgcg gagcttgtag tcgggccggc ctcgggacaa acagcctcgg cgtcctcccg
     3421 ccgcgctgcg gtgtccccgc ccgcgcgccc gccgcacacc ttccgcgccc agcgtgagtc
     3481 gcgcacctgc tctcgtctag acctgaccat ggacgatctg tttcccctca tctttccctc
     3541 agagccagcc caggcttctg ggccttatgt ggagatcatc gaacagccga agcaacgggg
     3601 catgcgattc cgctataaat gcgaggggcg ctcagcgggc agtattcctg gcgagagaag
     3661 cacagatacc accaagacac accccaccat caagatcaat ggctacacag gaccaggaac
     3721 agttcgaatc tccctggtca ccaaggatcc acctcaccgg cctcatccac atgaacttgt
     3781 ggggaaggac tgccgggatg gctactatga ggctgacctc tgcccagacc gcagtatcca
     3841 tagcttccag aacctgggga tccagtgtgt gaagaagcga gacctggagc aagccattag
     3901 ccagcgaatc cagaccaaca ataacccctt tcacgttcct atagaggagc agcgcgggga
     3961 ctatgacttg aatgcagtgc gcctctgctt ccaggtgaca gtgcgggacc cagcaggcag
     4021 gcccctcctc ctgacccctg tcctctcaca tccgattttt gataaccggg cccccaacac
     4081 tgccgagctc aagatctgcc gagtaaaccg gaactctggg agctgcctcg gtggggatga
     4141 gatcttcttg ctgtgcgaca aggtgcagaa agaagacatt gaggtgtatt tcacgggacc
     4201 aggctgggag gcacgaggct ccttttctca agctgatgtg catcggcaag tggccattgt
     4261 gttccggact cctccgtacg ccgaccccag cctccaggct cctgttcgag tctccatgca
     4321 gctacggcgg ccttctgatc gcgagctcag tgagcccatg gagttccagt acttgccaga
     4381 cacagatgat cgccaccgga ttgaagagaa gcgcaaaagg acctatgaga ccttcaagag
     4441 tatcatgaag aagagtcctt tcaatggacc aactgaaccc cggcctccaa cccggcgtat
     4501 tgctgtgcct acccgaaact caacttctgt ccccaagcca gccccgcagc cctacacctt
     4561 cccagcatcc ctcagcacca tcaactttga tgagttttcc cccatgctgt taccatcagg
     4621 gcagatctca aaccaggccc tggccttagc accgtcctct gccccagtcc ttgcccagac
     4681 catggtccct tcctcagcca tggtacctct ggctcagccc ccagctcctg ccccagttct
     4741 aaccccgggt cctccccagt ccctgtctgc acctgttcca aagagcaccc aggctgggga
     4801 aggcacgctg tcggaagccc tgctgcacct gcagtttgat gctgatgaag acttgggggc
     4861 cttgcttggc aacagcacag acccaggagt gttcacagac ctggcatctg tggacaactc
     4921 agagtttcag cagctcctga accagggtgt gtccatgtct cactccacag ctgagcccat
     4981 gctgatggag taccctgaag ctataactcg cctggtgaca gggtcccaga ggccccctga
     5041 cccagctccc acacccctgg ggacctcggg gcttcccaat ggtctctccg gagatgaaga
     5101 cttctcctcc attgcggaca tggacttctc tgctcttttg agtcagatca gctccaccca
     5161 tgggcatggc accggcagca ccggcagcgg cagctcatcc ggaactagta tggtgagcaa
     5221 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5281 cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
     5341 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5401 cttcggctac ggcgtggcct gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5461 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt tcaaggacga
     5521 cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5581 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5641 caacttcaac agccacaacg tctatatcac ggccgacaag cagaagaacg gcatcaaggc
     5701 taacttcaag atccgccaca acgttgagga cggcagcgtg cagctcgccg accactacca
     5761 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcca
     5821 tcagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5881 cgtgaccgcc gcctaagcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag
     5941 tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
     6001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6361 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6481 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6541 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa
     6601 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     6661 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     6721 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     6781 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     6841 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     6901 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     6961 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     7021 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     7081 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7141 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7201 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7261 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7321 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7381 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7441 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7501 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7561 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7621 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7681 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     7741 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     7801 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     7861 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7921 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7981 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8041 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8101 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8161 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8221 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8401 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     8461 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8521 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8581 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8641 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8701 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8761 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8821 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8881 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8941 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9001 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9061 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     9121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9241 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9301 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9361 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9421 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9481 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9541 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9601 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9661 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9721 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9781 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9841 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10081 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10141 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10201 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10261 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10321 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10381 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10441 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10501 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10561 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10621 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10681 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10741 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10801 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10861 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10921 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10981 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11041 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    11101 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11161 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11221 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    11281 actaaaggga acaaaagctg gagctgcaag cttaa
//
