LOCUS       sequence-244621-        1959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff 
            MJ, Danev R, Sexton PM, Wootten D, Gellman SH
  TITLE     Structural and functional diversity among agonist-bound states of 
            the GLP-1 receptor.
  JOURNAL   Nat Chem Biol. 2022 Mar;18(3):256-263. doi: 
            10.1038/s41589-021-00945-w. Epub 2021 Dec 22.
  PUBMED    34937906
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00945-w"; journalName: "Nat Chem Biol"; date: 
            "2022-03"; volume: "18"; issue: "3"; pages: "256-263"
COMMENT     Sequence Label: nLuc-GLP-1R-His-FLAG
FEATURES             Location/Qualifiers
     source          1..1959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..579
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1915..1932
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1933..1956
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggg
       61 gatggtatgg tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac
      121 aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg
      181 tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc
      241 catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt
      301 tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca
      361 ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc
      421 atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa
      481 attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac
      541 ggagtgaccg gctggcggct gtgcgaacgt attctggcgg gtggtcctcg ccctcagggc
      601 gctaccgtgt ccctctggga aaccgtccag aagtggaggg agtaccgtag acaatgccag
      661 cgtagcttga ccgaggaccc tcctcccgcg acagacctct tctgtaacag gacgttcgat
      721 gaatacgcat gctggcctga tggagagccg ggctcattcg ttaacgtcag ctgtccgtgg
      781 tatctgcctt gggcttcctc tgttccccag ggtcatgtct accgcttctg tacggcagaa
      841 ggtctgtggc tccaaaaaga caacagttcg ttgccttggc gcgacctgtc cgaatgcgag
      901 gaatcaaaaa gaggcgaacg ctcgtctcca gaggaacaac tgctgttcct ttacattatc
      961 tacacggtag gatacgccct ttctttctcg gcgctcgtaa tcgcctcggc gatcctgctt
     1021 ggctttcgcc acctgcactg cactaggaac tacattcacc tgaatttgtt cgcttcattc
     1081 atcttgcgtg ccctctcagt tttcattaag gacgccgctc ttaagtggat gtacagcaca
     1141 gctgctcaac agcatcagtg ggacggtctg ttgtcctacc aggacagtct gagttgtcgt
     1201 ctggtcttcc tgctgatgca gtattgtgtc gcagcaaact actattggct cttggttgag
     1261 ggtgtctacc tgtacactct tttggccttc agcgtgtttt ctgagcagtg gatcttcaga
     1321 ttgtacgttt ccatcggctg gggagtgccc cttctctttg ttgtgccttg gggcatagtt
     1381 aagtaccttt acgaggatga aggttgctgg acaagaaatt ctaatatgaa ctactggctg
     1441 attatccgtc tgcctattct ctttgccatt ggtgtaaact ttttgatctt cgtaagggta
     1501 atctgcatag tcgtatctaa gttgaaggct aacttgatgt gtaaaaccga tatcaagtgt
     1561 cgcctggcca agtcaacact cactttgatc ccccttctgg gtacgcacga ggtaatcttt
     1621 gcgtttgtca tggacgaaca cgcccgtggt actctccgct tcatcaagtt gtttaccgag
     1681 ttgtccttta ccagtttcca aggactcatg gtggccattc tgtactgctt cgtcaataac
     1741 gaggtccagt tggagttccg caagtcatgg gaacgctggc gcctggaaca cctgcatatc
     1801 cagcgcgact cctctatgaa accgctgaag tgtccaacca gttcattgtc atccggagct
     1861 actgcgggat cttccatgta taccgccact tgtcaggctt catgttctct cgagcatcat
     1921 caccaccacc acgactataa ggacgatgat gacaagtaa
//
