LOCUS       sequence-244665-       10696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For general lentiviral or direct expression of cytosolic jRGECO1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou
            S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
  TITLE     Wide-area all-optical neurophysiology in acute brain slices.
  JOURNAL   J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019.
  PUBMED    30952812
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019."
COMMENT     Sequence Label: SLF063: Lenti-fCAG-jRGECO1a
FEATURES             Location/Qualifiers
     source          1..10696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     495..517
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     579..598
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             731..748
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             752..784
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(788..796,797..811)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             839..895
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1591..1610
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2102..2690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2155..2175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2573..2584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2693..2709)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2694..2710)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3215..3395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3421..3438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3427..3651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3697..4125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3784..3803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3994..4015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4134..4154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4139..4468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4319..4454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4381..4400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4516..4563
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4582..4956
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5086..5207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5123..5142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5177..5196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5256..5272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5256..5272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5269..5291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5280..5296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5304..5310,5311..5328,5329..5334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5349..5370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5487..5504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5658..6246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5738..5757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6417..7208,7209..7277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7040..7059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7278..7382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7457..7476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7648..8027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8029..8227
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8182..8202
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8245..8425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8472..8597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9090..9323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9508..9552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9850..9967
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10083..10386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggg ggcgcgcccc cccccccccc ccccccccgc cggcgggggg
       61 cggggcgggg gggggggggg gggggggggg gggggggggg gggggggggc gggggggggg
      121 gggggggggg gggggggggg ggcgcggggg gggggggccg cctcgggccg gggagggctc
      181 gggggagggg cgcggcggtc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
      241 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
      301 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
      361 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
      421 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
      481 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
      541 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
      601 gtctcatcat tttggcaaag aattgatttg ataccgcggg cccgggatct cgaggatcgc
      661 caccatgctg cagaacgagc ttgctcttaa gttggctgga cttgatatta acaagactgg
      721 aggaggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      781 gggtcgggat ctgtacgacg atgacgataa ggatctcgca acaatggtcg actcatcacg
      841 tcgtaagtgg aataaggcag gtcacgcagt cagagctata ggtcggctga gctcacccgt
      901 ggtttccgag cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaaggggct
      961 gaggctgaag gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa
     1021 gcccgtgcag ctgcccggcg cctacatcgt ggacatcaag ttggacatcg tgtcccacaa
     1081 cgaggactac accatcgtgg aacagtgcga acgcgccgag ggccgccact ccaccggcgg
     1141 catggacgag ctgtacaagg gaggtacagg cgggagtctg gtgagcaagg gcgaggagga
     1201 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1261 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac
     1321 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1381 tcagttcatg tacggctcca aggcctacat taagcaccca gccgacatcc ccgactactt
     1441 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg
     1501 cattattcac gtgaaccagg actcctccct gcaggacggc gtattcatct acaaggtgaa
     1561 gctgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1621 ggaggctacg cgtgacgacc tgactgaaga gcagatcgca gaatttaaag aggctttctc
     1681 cctatttgac aaggacgggg atgggacgat aacaaccaag gagcttggga cggtgttccg
     1741 atcgctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg aagtagatgc
     1801 cgacggtgac ggcacattcg acttccctga gttcctgacg atgatggcaa gaaaaatgaa
     1861 tgacacagac agtgaagagg aaattcgcga agcgttccgc gtgtttgata aggacggcaa
     1921 tggctacatc ggcgcagcag agcttcgcca cgtgatgaca gaccttggag agaagttaac
     1981 agatgaggag gttgatgaaa tgatcagggt agcagacatc gatggggatg gtcaggtaaa
     2041 ctacgaagag tttgtacaaa tgatgacagc gaagtaggaa ttcgatatca agcttatcga
     2101 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2461 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     2701 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     2761 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     2821 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2881 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     2941 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     3001 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     3061 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     3121 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     3181 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     3241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3361 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     3421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3901 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3961 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4021 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4081 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4141 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4201 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4261 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4381 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4441 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4501 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     4561 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     4621 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     4681 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     4741 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     4801 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     4861 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     4921 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     4981 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5041 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5101 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5221 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5281 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5341 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5401 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5461 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7381 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     7441 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     7501 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     7561 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     7621 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     7681 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     7741 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     7801 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     7861 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     7921 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     7981 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     8041 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     8101 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     8161 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     8221 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     8281 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8341 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8401 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8461 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8521 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8581 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8641 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8701 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8761 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8821 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8881 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8941 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     9001 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     9061 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     9121 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     9181 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     9241 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     9301 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     9361 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9421 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9481 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9541 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9601 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9661 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9721 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     9781 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     9841 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9901 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9961 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta actgcaggaa
    10021 tctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    10081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    10141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    10201 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    10261 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    10321 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    10381 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
    10441 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
    10501 ggggggcgcg cgccaggcgg ggcggggggg gggggggggg gggggggggg gggggggggg
    10561 gggggggggg gggccaacag gggggggcgc gcccgaaggt ttctcttttt tggggggggg
    10621 gggggggggg ggcggcccaa aaaaaagaga gcgcgggggg gggggggggg ggcgcgcgcg
    10681 cgcccccccc cccccc
//
