LOCUS       sequence-244674-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox 
            inducible in TetR cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, 
            Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley 
            JF, Morris JR
  TITLE     The deSUMOylase SENP2 coordinates homologous recombination and 
            nonhomologous end joining by independent mechanisms.
  JOURNAL   Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. 
            Epub 2019 Feb 22.
  PUBMED    30796017
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-03-1"; volume: "33(5-6):333-347. doi"; pages: " 
            10.1101/gad.321125.118. Epub 2019 Feb 22"
COMMENT     Sequence Label: FLAG-SENP2 WT
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..48
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    71..89
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     99..123
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             223..246
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
       61 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
      121 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      181 agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa ggacgacgat
      241 gacaagatcg attacagatg gctggttagg attctcggca ccattttccg tttctgcgac
      301 cggtcggtgc cccctgcccg ggccctcctg aagaggcggc gctcagacag cactctgttt
      361 tctacagtgg acactgatga aataccagcc aaaagaccaa gattagattg ctttattcac
      421 caagtgaaaa acagtctcta caatgctgcc agcttatttg gattcccatt ccagctgacc
      481 acaaagccca tggtaacttc tgcttgtaat ggaacacgga atgtggcccc ttcaggagag
      541 gtattttcga actcttcatc ttgtgaactg acaggttctg gatcctggaa caacatgctg
      601 aaactgggta ataaatctcc taatggaata agtgactatc caaagatcag agtgacagtt
      661 acccgagatc agccacgcag agtcctgcct tcctttggtt ttactttgaa ctcagaaggc
      721 tgtaatagaa gaccaggtgg ccgtcgccat agcaaaggta atccagagag ttctttaatg
      781 tggaaacctc aggaacaggc tgtaacagag atgatttctg aagagagtgg caagggtctg
      841 aggcgtcccc attgtactgt ggaggagggt gttcaaaaag aggaaagaga gaagtaccga
      901 aagttattgg aacgacttaa agaaagtggt catggaaact ctgtctgtcc tgtaacttca
      961 nattatcaca gttctcaaag aagtcagatg ganacattaa cgaccaaagg ttggtgggaa
     1021 gagcatantc accgagtcat accanctcnn tttgctccaa acantataca ctnttgaaa
//
