LOCUS       sequence-244678-        1331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian 
            cells, Dox inducible in TetR cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, 
            Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley 
            JF, Morris JR
  TITLE     The deSUMOylase SENP2 coordinates homologous recombination and 
            nonhomologous end joining by independent mechanisms.
  JOURNAL   Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. 
            Epub 2019 Feb 22.
  PUBMED    30796017
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-03-1"; volume: "33(5-6):333-347. doi"; pages: " 
            10.1101/gad.321125.118. Epub 2019 Feb 22"
COMMENT     Sequence Label: FLAG-SENP2 NPm
FEATURES             Location/Qualifiers
     source          1..1331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..49
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     100..124
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             224..247
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgcaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
       61 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
      121 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      181 cagcctccgg actctagcgt ttaaacttaa gcttggtacc atggactaca aggacgacga
      241 tgacaagatc gatgctgcca gcttatttgg attcccattc cagctgacca caaagcccat
      301 ggtaacttct gcttgtaatg gaacacggaa tgtggcccct tcaggagagg tattttcgaa
      361 ctcttcatct tgtgaactga caggttctgg atcctggaac aacatgctga aactgggtaa
      421 taaatctcct aatggaataa gtgactatcc aaagatcaga gtgacagtta cccgagatca
      481 gccacgcaga gtcctgcctt cctttggttt tactttgaac tcagaaggct gtaatagaag
      541 accaggtggc cgtcgccata gcaaaggtaa tccagagagt tctttaatgt ggaaacctca
      601 ggaacaggct gtaacagaga tgatttctga agagagtggc aagggtctga ggcgtcccca
      661 ttgtactgtg gaggagggtg ttcaaaaaga ggaaagagag aagtaccgaa agttattgga
      721 acgacttaaa gaaagtggtc atggaaactc tgtctgtcct gtaacttcaa attatcacag
      781 ttctcaaaga agtcagatgg acacattaaa gaccaaaggc tggggggaag agcaaaatca
      841 cggagtcaaa acaactcagt ttgttccaaa acaatataga cttgttgaaa caaggggacc
      901 tctatgttca ttgagaagtg aaaagaggtg ttcaaagggg aaaattactg atacagagac
      961 gatggtcgga atcagatttg aaaatgaaag taggagggga taccaactgg agcctgacct
     1021 atcagaanaa gtgtcggccc gagcccgcgc gggcagtgga agcaatggct tactcaggag
     1081 gaaagtgtca ataattgnna caaaggaaaa naattgctca ggcaaanaga ggganngaag
     1141 aacggacgat ctccttgaac ttancgagga nntggaaaag gaaatcagta atgccctagg
     1201 cctggcccnn nggatgaaat cctaagtann gctttcaaat tgnnaattac tncaggaaac
     1261 ttcaaacttt aagaacnttc ctggnncnng nnngaannct taatttttac tgaannnnng
     1321 ggggaaagaa a
//
