LOCUS       sequence-244680-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian 
            cells, Dox inducible in TetR cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, 
            Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley 
            JF, Morris JR
  TITLE     The deSUMOylase SENP2 coordinates homologous recombination and 
            nonhomologous end joining by independent mechanisms.
  JOURNAL   Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. 
            Epub 2019 Feb 22.
  PUBMED    30796017
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-03-1"; volume: "33(5-6):333-347. doi"; pages: " 
            10.1101/gad.321125.118. Epub 2019 Feb 22"
COMMENT     Sequence Label: FLAG-SENP2 CCm
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..52
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     103..127
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             227..250
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tnncgcaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
       61 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
      121 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      181 atccagcctc cggactctag cgtttaaact taagcttggt accatggact acaaggacga
      241 cgatgacaag atcgattaca gatggctggt taggattctc ggcaccattt tccgtttctg
      301 cgaccggtcg gtgccccctg cccgggccct cctgaagagg cggcgctcag acagcactct
      361 gttttctaca gtggacactg atgaaatacc agccaaaaga ccaagattag attgctttat
      421 tcaccaagtg aaaaacagtc tctacaatgc tgccagctta tttggattcc cattccagct
      481 gaccacaaag cccatggtaa cttctgcttg taatggaaca cggaatgtgg ccccttcagg
      541 agaggtattt tcgaactctt catcttgtga actgacaggt tctggatcct ggaacaacat
      601 gctgaaactg ggtaataaat ctcctaatgg aataagtgac tatccaaaga tcagagtgac
      661 agttacccga gatcagccac gcagagtcct gccttccttt ggttttactt tgaactcaga
      721 aggctgtaat agaagaccag gtggccgtcg ccatagcaaa ggtaatccag agagttcttt
      781 aatgtggaaa cctcaggaac aggctgtaac agagatgatt tctgaagaga gtggcaaggg
      841 tctgaggcgt ccccattgtg gaaactctgt ctgtcctgta acttcaaatt atcacagttc
      901 tcaaagaagt cagatggaca cattaaagac caaaggctgg ggggaagagc aaaatcacgg
      961 agtcaaaaca actcagtttg ttccaaaaca atatagactt gttgaaacaa ggggacctct
     1021 atgttcattg agaagtgaaa agaggtgttc aaaggggaaa attactgata cagagacgat
     1081 ggtcggaatc agatttgaaa atgaaagtag gaggggatac caactggagc ctgacctatc
     1141 anaagaagtg tcggcccgac tccgcctggg cagtggaagc aatggcttac tcaggaggaa
     1201 agtgtcaata attgagacaa aggaaaagaa ttgctcaggc aangagaggg acagannaac
     1261 ggacgatctc cttgaactta cngagganat gggaaaagga aatcagtaat gccctaggcc
     1321 atggcccaan ggatgaaatc ctaagtagtg ctttcaaatt gnnaattact cgaggaaann
     1381 tacaaacatt aaagaactat cnctggnctc atggnnnaag t
//
