LOCUS       sequence-244685-       10127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human G9a mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Wang L, Xu S, Lee JE, Baldridge A, Grullon S, Peng W, Ge K
  TITLE     Histone H3K9 methyltransferase G9a represses PPARgamma expression 
            and adipogenesis.
  JOURNAL   EMBO J. 2012 Nov 23. doi: 10.1038/emboj.2012.306.
  PUBMED    23178591
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2012 Nov 23. doi: 10.1038/emboj.2012.306."
COMMENT     Sequence Label: MSCVhygro-F-G9adSET
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4620..5119
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4655..4678)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5052..5071
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5137..6162
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5137..6162
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6520..7034
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7220..7226,7227..7244,7245..7250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7403..7420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7574..8162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7654..7673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8333..9124,9125..9193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8956..8975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9194..9298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9366..9384
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9422..9444)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9544..9563
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9757..9779
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9907..9926
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(10126..10127,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatcctggcc accatggact acaaggacga
     1441 cgatgacaag atggcggcgg cggcgggagc tgcagcggcg gcggccgccg agggggaggc
     1501 ccccgctgag atgggggcgc tgctgctgga gaaggaaacc agaggagcca ccgagagagt
     1561 tcatggctct ttgggggaca cccctcgtag tgaagaaacc ctgcccaagg ccaaccccga
     1621 ctccctggag cctgctggcc cctcatctcc agcctctgtc actgtcactg ttggtgatga
     1681 gggggctgac acccctgtag gggctacacc actcattggg gatgaatctg agaatcttga
     1741 gggagatggg gacctccgtg ggggccgcat cctgctgggc catgccacaa agtcattccc
     1801 ctcttccccc agcaaggggg gttcctgccc tagccgggcc aagatgtcaa tgacaggggc
     1861 gggaaaatca cctccatctg tccagagttt ggctatgagg ctactgagta tgccaggagc
     1921 ccagggagct gcagcagcag ggtctgaacc ccctccagcc accacgagcc cagagggaca
     1981 gcccaaggtc caccgagccc gcaaaaccat gtccaaacca ggaaatggac agcccccggt
     2041 ccctgagaag cggccccctg aaatacagca tttccgcatg agtgatgatg tccactcact
     2101 gggaaaggtg acctcagatc tggccaaaag gaggaagctg aactcaggag gtggcctgtc
     2161 ggaggagtta ggttctgccc ggcgttcagg agaagtgacc ctgacgaaag gggaccccgg
     2221 gtccctggag gagtgggaga cggtggtggg tgatgacttc agtctctact atgattccta
     2281 ctctgtggat gagcgcgtgg actccgacag caagtctgaa gttgaagctc taactgaaca
     2341 actaagtgaa gaggaggagg aggaagagga ggaagaagaa gaagaggaag aggaggagga
     2401 agaggaagaa gaagaggaag atgaggagtc agggaatcag tcagatagga gtggttccag
     2461 tggccggcgc aaggccaaga agaaatggcg aaaagacagc ccatgggtga agccgtctcg
     2521 gaaacggcgc aagcgggagc ctccgcgggc caaggagcca cgaggagtga atggtgtggg
     2581 ctcctcaggc cccagtgagt acatggaggc ccctctgggg tccctggagc tgcccagcga
     2641 ggggaccctc tcccccaacc acgctggggt gtccaatgac acatcttcgc tggagacaga
     2701 gcgagggttt gaggagttgc ccctgtgcag ctgccgcatg gaggcaccca agattgaccg
     2761 catcagcgag agggcggggc acaagtgcat ggccactgag agtgtggacg gagagctgtc
     2821 aggctgcaat gccgccatcc tcaagcggga gaccatgagg ccatccagcc gtgtggccct
     2881 gatggtgctc tgtgagaccc accgcgcccg catggtcaaa caccactgct gcccgggctg
     2941 cggctacttc tgcacggcgg gcaccttcct ggagtgccac cctgacttcc gtgtggccca
     3001 ccgcttccac aaggcctgtg tgtctcagct gaatgggatg gtcttctgtc cccactgtgg
     3061 ggaggatgct tctgaagctc aagaggtgac catcccccgg ggtgacgggg tgaccccacc
     3121 ggccggcact gcagctcctg cacccccacc cctgtcccag gatgtccccg ggagagcagg
     3181 cacttctcag cccagtgccc ggatgcgagg gcatggggaa ccccggcgcc cgccctgcga
     3241 tcccctggct gacaccattg acagctcagg gccctccctg accctgccca atgggggctg
     3301 cctttcagcc gtggggctgc cactggggcc aggccgggag gccctggaaa aggccctggt
     3361 catccaggag tcagagaggc ggaagaagct ccgtttccac cctcggcagt tgtacctgtc
     3421 cgtgaagcag ggcgagctgc agaaggtgat cctgatgctg ttggacaacc tggaccccaa
     3481 cttccagagc gaccagcaga gcaagcgcac gcccctgcat gcagccgccc agaagggctc
     3541 cgtggagatc tgccatgtgc tgctgcaggc tggagccaac ataaatgcag tggacaaaca
     3601 gcagcggacg ccactgatgg aggccgtggt gaacaaccac ctggaggtag cccgttacat
     3661 ggtgcagcgt ggtggctgtg tctatagcaa ggaggaggac ggttccacct gcctccacca
     3721 cgcagccaaa atcgggaact tggagatggt cagcctgctg ctgagcacag gacaggtgga
     3781 cgtcaacgcc caggacagtg gggggtggac gcccatcatc tgggctgcag agcacaagca
     3841 catcgaggtg atccgcatgc tactgacgcg gggcgccgac gtcaccctca ctgacaacga
     3901 ggagaacatc tgcctgcact gggcctcctt cacgggcagc gccgccatcg ccgaagtcct
     3961 tctgaatgcg cgctgtgacc tccatgctgt caactaccat ggggacaccc ccctgcacat
     4021 cgcagctcgg gagagctacc atgactgcgt gctgttattc ctgtcacgtg gggccaaccc
     4081 tgagctgcgg aacaaagagg gggacacagc atgggacctg actcccgagc gctccgacgt
     4141 gtggtttgcg cttcaactca accgcaagct ccgacttggg gtgggaaatc gggccatccg
     4201 cacagagaag atcatctgcc gggacgtggc tcggggctat gagaacgtgc ccattccctg
     4261 tgtcaacggt gtggatgggg agccctgccc tgaggattac aagtacatct cagagaactg
     4321 cgagacgtcc accatgaaca tcgatcgcaa catcacccac ctgcagcact gcacgtgtgt
     4381 ggacgactgc tctagctcca actgcctgtg cggccagctc agcatccggt gctggtatga
     4441 caaggatggg cgattgctcc aggaatttaa caagattgag cctccgctga ttttcgagtg
     4501 taaccaggcg tgctcatgct ggagaaactg caagaaccgg gtcgtacaga gtggcatcaa
     4561 ggtgcggcta cagctctacc gaacagccaa gatgtgactc gaggttaacg aattctaccg
     4621 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4681 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4741 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4801 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4861 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     4921 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     4981 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5041 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5101 ctcatctccg ggcctttcga cctgcatccc gccaccatga aaaagcctga actcaccgcg
     5161 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5221 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5281 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     5341 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     5401 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     5461 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     5521 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     5581 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     5641 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     5701 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     5761 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     5821 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     5881 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     5941 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6001 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     6061 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6121 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac
     6181 aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc
     6241 gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg
     6301 gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc
     6361 agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat
     6421 cgccgcccgt gcctgccgat tgggtgcaga tccgtcgacc tgcagccaag cttatcgata
     6481 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     6541 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa
     6601 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat
     6661 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     6721 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     6781 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     6841 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     6901 tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     6961 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     7021 cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga
     7081 gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata
     7141 ctcctgaaat agttcattat ggacagcgca gaaagagctg gggagaattg tgaaattgtt
     7201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9301 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9361 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9421 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9481 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9541 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9601 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9661 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9721 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9781 cggcgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     9841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     9901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     9961 ggccacgatg cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt
    10021 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
    10081 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaa
//
