LOCUS       sequence-244688-        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
  TITLE     Disruption of the Scaffolding Function of mLST8 Selectively Inhibits
            mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor 
            Growth In Vivo.
  JOURNAL   Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 
            10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13.
  PUBMED    31085701
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-3658"; journalName: "Cancer Res"; date: 
            "2019-07-1- 1"; volume: "79"; issue: "13"; pages: "3178-3184"
COMMENT     Sequence Label: pLentiCMV-Blast mLST8-Flag Mut1
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    160..276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        299..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     803..827
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1867..1890
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1901..2489
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1954..1974)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2372..2383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2540..2560)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2545..2874
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2725..2860
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2922..2969
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2988..3386
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3473..3706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3778..3899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3815..3834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3869..3888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3939..4074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4094..4113)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4095..4113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4123..4140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4123..4139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4132..4154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4281..4736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4368..4387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4578..4599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4762..4866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4867..4935,4936..5727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5085..5104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5898..6486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6387..6406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6640..6657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6774..6795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6810..6815,6816..6833,6834..6840)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6848..6864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6853..6875
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6872..6888
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6872..6888
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6907..6927
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6909..6927
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6955..7181
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7182..7362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7409..7534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8027..8260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8445..8489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
       61 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
      121 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
      181 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      241 agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
      841 atccacgctg ttttgacctc catagaagac accgactcta gagccaccat gaacacctcc
      901 ccaggcacgg tgggcagtga cccggtcatc ctggcaaccg ccggctacga ccacaccgtg
      961 cgcttctggc aggcccacag cggcatctgc acccggacgg tgcagcacca ggactccgag
     1021 gtgctggcct tggaggtcac accggaccgc agcatgattg ctgctgcagg ttaccagcac
     1081 atccgcatgt atgatctcaa ctccaataac cctaacccca tcatcagcta cgacggcgtc
     1141 aacaagaaca tcgcgtctgt gggcttccac gaagacggcc gctggatgta cacgggcggc
     1201 gaggactgca cagccaggat ctgggacctc aggtcccgga acctgcagtg ccagcggatc
     1261 ttccaggtga acgcacccat taactgcgtg tgcctgcacc ccaaccaggc agagctcatc
     1321 gtgggtgacc agagcggggc tatccacatc tgggacttga aaacagacca caacgagcag
     1381 ctgatccctg agcccgaggt ctccatcacg tccgcccaca tcgatcccga cgccagctac
     1441 atggcagctg tcaatagcac cggaaactgc tatgtctgga atctgaccgg tggcattggt
     1501 gacgaggtga cccagctcat ccccaagact aagatccctg cccacacgcg ctacgccctg
     1561 cagtgtcgct tcagccccga ctccacgctc ctcgccacct gctcggctga tcagacgtgc
     1621 aagatctgga ggacgtccaa cttctccctg atgacggagc tgagcatcaa gagcggcaac
     1681 cccggggagt cctcccgcgg ctggatgtgg ggctgcgcct tctcggggga ctcccagtac
     1741 atcgtcactg cttcctcgga caacctggcc cggctctggt gtgtggagac tggagagatc
     1801 aagagagagt atggcggcca ccagaaggct gttgtctgcc tggccttcaa tgacagtgtg
     1861 ctgggcgact acaaagacga tgacgacaag taatgtcgac aatcaacctc tggattacaa
     1921 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1981 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2041 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2101 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2161 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2221 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2281 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     2341 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2401 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2461 tcggatctcc ctttgggccg cctccccgcc tggaattctg cagatatccg gttagtaatg
     2521 agtttggaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2581 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2641 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2701 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2761 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     2821 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2881 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     2941 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
     3001 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     3061 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     3121 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     3181 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     3241 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     3301 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     3361 cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt aagaccaatg
     3421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3481 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3721 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3901 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3961 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     4081 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4141 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4201 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4261 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4321 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4381 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4441 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4501 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4561 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4621 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4681 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4741 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4801 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4861 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5101 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5161 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5401 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5701 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6781 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6841 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6901 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6961 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     7021 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     7081 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7141 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7381 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7441 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7501 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7561 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7621 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7681 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7741 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7801 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7861 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8581 ctgtactttc tatagt
//
