LOCUS       sequence-244689-        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stable expression_ sgRNA resistant Flag tagging mLST8 
            W272F/W274F mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
  TITLE     Disruption of the Scaffolding Function of mLST8 Selectively Inhibits
            mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor 
            Growth In Vivo.
  JOURNAL   Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 
            10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13.
  PUBMED    31085701
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-3658"; journalName: "Cancer Res"; date: 
            "2019-07-1- 1"; volume: "79"; issue: "13"; pages: "3178-3184"
COMMENT     Sequence Label: pLentiCMV-Blast mLST8-Flag Mut2
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    147..263
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        286..589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        590..793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     744..764
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     790..814
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1854..1877
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1888..2476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1941..1961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2359..2370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2527..2547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2532..2861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2712..2847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2909..2956
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2975..3373
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3460..3693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3765..3886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3802..3821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3856..3875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3926..4061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4081..4100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4082..4100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4110..4127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4110..4126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4268..4723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4355..4374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4565..4586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4749..4853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4854..4922,4923..5714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5072..5091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5885..6473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6374..6393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6627..6644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6761..6782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6797..6802,6803..6820,6821..6827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6835..6851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6840..6862
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6859..6875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6859..6875
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6894..6914
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6896..6914
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6942..7168
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7169..7349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7396..7521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8014..8247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8432..8476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
       61 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      121 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
      181 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      241 caaattacaa aattcaaaat tttatcgata agcttgggag ttccgcgtta cataacttac
      301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      601 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      661 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      721 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      781 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      841 tgacctccat agaagacacc gactctagag ccaccatgaa cacctcccca ggcacggtgg
      901 gcagtgaccc ggtcatcctg gcaaccgccg gctacgacca caccgtgcgc ttctggcagg
      961 cccacagcgg catctgcacc cggacggtgc agcaccagga ctcccaggtg aatgccttgg
     1021 aggtcacacc ggaccgcagc atgattgctg ctgcaggtta ccagcacatc cgcatgtatg
     1081 atctcaactc caataaccct aaccccatca tcagctacga cggcgtcaac aagaacatcg
     1141 cgtctgtggg cttccacgaa gacggccgct ggatgtacac gggcggcgag gactgcacag
     1201 ccaggatctg ggacctcagg tcccggaacc tgcagtgcca gcggatcttc caggtgaacg
     1261 cacccattaa ctgcgtgtgc ctgcacccca accaggcaga gctcatcgtg ggtgaccaga
     1321 gcggggctat ccacatctgg gacttgaaaa cagaccacaa cgagcagctg atccctgagc
     1381 ccgaggtctc catcacgtcc gcccacatcg atcccgacgc cagctacatg gcagctgtca
     1441 atagcaccgg aaactgctat gtctggaatc tgaccggtgg cattggtgac gaggtgaccc
     1501 agctcatccc caagactaag atccctgccc acacgcgcta cgccctgcag tgtcgcttca
     1561 gccccgactc cacgctcctc gccacctgct cggctgatca gacgtgcaag atctggagga
     1621 cgtccaactt ctccctgatg acggagctga gcatcaagag cggcaacccc ggggagtcct
     1681 cccgcggctt catgttcggc tgcgccttct cgggggactc ccagtacatc gtcactgctt
     1741 cctcggacaa cctggcccgg ctctggtgtg tggagactgg agagatcaag agagagtatg
     1801 gcggccacca gaaggctgtt gtctgcctgg ccttcaatga cagtgtgctg ggcgactaca
     1861 aagacgatga cgacaagtaa tgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     1921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2461 tgggccgcct ccccgcctgg aattctgcag atatccggtt agtaatgagt ttggaattaa
     2521 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2581 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2641 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2701 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2761 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     2821 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     2881 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     2941 catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga
     3001 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     3061 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     3121 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     3181 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     3241 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     3301 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     3361 gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     3421 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
     3481 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3661 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     3721 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3781 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3841 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3901 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4081 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4141 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4201 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4261 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4681 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4741 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4801 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4861 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4921 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4981 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5041 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5101 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5161 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5221 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5281 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5341 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5401 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5461 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5521 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5581 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5641 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5701 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5761 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5821 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6781 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6841 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6901 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6961 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7021 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7081 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7141 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7321 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7381 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7441 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7501 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7561 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7621 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7681 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7741 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7801 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7861 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7921 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7981 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8041 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8101 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8161 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8221 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8281 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8341 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8401 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8461 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8521 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8581 agtgaataga gttagg
//
