LOCUS       sequence-244692-        8596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L 
            and W272F/W274F mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
  TITLE     Disruption of the Scaffolding Function of mLST8 Selectively Inhibits
            mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor 
            Growth In Vivo.
  JOURNAL   Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 
            10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13.
  PUBMED    31085701
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-3658"; journalName: "Cancer Res"; date: 
            "2019-07-1- 1"; volume: "79"; issue: "13"; pages: "3178-3184"
COMMENT     Sequence Label: pLentiCMV-Blast mLST8-Flag Mut1-2
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    116..232
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        255..558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        559..762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     713..733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     759..783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1823..1846
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1857..2445
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1910..1930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2328..2339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2496..2516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2501..2830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2681..2816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2878..2925
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2944..3342
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3429..3662
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3734..3855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3771..3790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3825..3844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3895..4030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4050..4069)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4051..4069)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4079..4096)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4079..4095)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4088..4110)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4237..4692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4324..4343)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4534..4555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4718..4822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4823..4891,4892..5683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5041..5060)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5854..6442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6343..6362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6596..6613
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6730..6751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6766..6771,6772..6789,6790..6796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6804..6820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6809..6831
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6828..6844
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6828..6844
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6863..6883
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6865..6883
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6911..7137
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7138..7318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7365..7490
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7983..8216
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8401..8445
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
       61 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
      121 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      181 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgataa
      241 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      301 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      361 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      421 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      481 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      541 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      601 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      661 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      721 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      781 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagagc
      841 caccatgaac acctccccag gcacggtggg cagtgacccg gtcatcctgg caaccgccgg
      901 ctacgaccac accgtgcgct tctggcaggc ccacagcggc atctgcaccc ggacggtgca
      961 gcaccaggac tccgaggtgc tggccttgga ggtcacaccg gaccgcagca tgattgctgc
     1021 tgcaggttac cagcacatcc gcatgtatga tctcaactcc aataacccta accccatcat
     1081 cagctacgac ggcgtcaaca agaacatcgc gtctgtgggc ttccacgaag acggccgctg
     1141 gatgtacacg ggcggcgagg actgcacagc caggatctgg gacctcaggt cccggaacct
     1201 gcagtgccag cggatcttcc aggtgaacgc acccattaac tgcgtgtgcc tgcaccccaa
     1261 ccaggcagag ctcatcgtgg gtgaccagag cggggctatc cacatctggg acttgaaaac
     1321 agaccacaac gagcagctga tccctgagcc cgaggtctcc atcacgtccg cccacatcga
     1381 tcccgacgcc agctacatgg cagctgtcaa tagcaccgga aactgctatg tctggaatct
     1441 gaccggtggc attggtgacg aggtgaccca gctcatcccc aagactaaga tccctgccca
     1501 cacgcgctac gccctgcagt gtcgcttcag ccccgactcc acgctcctcg ccacctgctc
     1561 ggctgatcag acgtgcaaga tctggaggac gtccaacttc tccctgatga cggagctgag
     1621 catcaagagc ggcaaccccg gggagtcctc ccgcggcttc atgttcggct gcgccttctc
     1681 gggggactcc cagtacatcg tcactgcttc ctcggacaac ctggcccggc tctggtgtgt
     1741 ggagactgga gagatcaaga gagagtatgg cggccaccag aaggctgttg tctgcctggc
     1801 cttcaatgac agtgtgctgg gcgactacaa agacgatgac gacaagtaat gtcgacaatc
     1861 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1921 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1981 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2041 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2101 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2161 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2221 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     2281 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2341 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2401 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctgcaga
     2461 tatccggtta gtaatgagtt tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2761 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2821 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     2881 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     2941 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     3001 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     3061 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     3121 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     3181 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     3241 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     3301 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacaatt cgagctcggt
     3361 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3421 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     3481 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3541 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3601 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3661 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     3721 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3841 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     3901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4021 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4081 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4141 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4201 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4441 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4681 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6541 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6601 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6661 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6721 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6781 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6841 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     6901 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     6961 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     7021 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     7081 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7321 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7381 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7441 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7501 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7561 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7621 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7681 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7741 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7801 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7861 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7921 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7981 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8041 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8101 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8161 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8221 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8281 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8341 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8401 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8461 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8521 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8581 ttatcgtttc agaccc
//
