LOCUS       sequence-24470-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: Ta1098(243-339)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             351..368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(421..439)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      435..482
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cgnnattccc tctagaatat tttgtttaac tttaagaagg agatatacat atgagtaatg
       61 aaccnntngc ccgtgacaag gagatagtgg atgagtttct ggtcgcngtg aaaaaagata
      121 tgggggtcta tgggagggac cagactgaga gtgctctgca gatgggggcc ctgtcagacc
      181 tcataataac cgatgaaatg ttcagaacag aggatggcag aagatcactg agcattgctc
      241 aaacggttgg gacgagaata catatagtga gcgtgagcaa tgatccgggg cagatagtga
      301 agaaattcgg tggatttgca ggtattttgc gctacagggt gcagctcgag caccaccacc
      361 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      421 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      481 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      541 aaccgcggcg gntgtggtgg ttacccgcan cgtgaccgct acacttgccn gctccctagc
      601 gcccgctccc tntngctttc ntcccttcct ttctcgccac gntcgccggc tttcccgcna
      661 natncntaat cgggggcntg cctttagggt ccgatttagt gcttacnggc actcgacccc
      721 aaaacctgat aggtgatgnt cncgtagtgg gcntcgcctg naacgttttc gcctttgagt
      781 ggagcacgtc ttanntggnn ctgtcaaang ganacctcac ctntncgtct tcttgataat
      841 gattgcgatc gcttgtaaat ggcnttnaat tacnattaat
//
