LOCUS       sequence-244794-       12598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the full-length human TET1 gene with a Flag 
            and HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y, 
            Agarwal S, Iyer LM, Liu DR, Aravind L, Rao A
  TITLE     Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in 
            mammalian DNA by MLL partner TET1.
  JOURNAL   Science. 2009 May 15;324(5929):930-5. Epub 2009 Apr 16.
  PUBMED    19372391
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2009-05-15"; volume: "324(5929)"; pages: "930-5. Epub 2009 
            Apr 16"
COMMENT     Sequence Label: FH-TET1-pEF
FEATURES             Location/Qualifiers
     source          1..12598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        163..1341
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          394..1332
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1289..1309
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1358..1377
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1358..1376
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1409..1418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1418..1441
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1454..1480
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1454..1480
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7919..7960
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7970..7987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8010..8027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8016..8240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8286..8714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8373..8392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8583..8604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8723..8743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8728..9057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8908..9043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8970..8989
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9124..9918
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9178..9197)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9788..9807
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10094..10215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10131..10150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10185..10204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10264..10280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10264..10280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10277..10299)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10288..10304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10312..10318,10319..10336,10337..10342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10357..10378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10495..10512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10655..11243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10735..10754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11414..12205,12206..12274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12037..12056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12275..12379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12454..12473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
       61 actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
      121 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
      181 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      241 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      301 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      361 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
      421 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
      481 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
      541 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
      601 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
      661 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
      721 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
      781 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
      841 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
      901 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
      961 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     1021 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     1081 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     1141 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     1201 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     1261 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     1321 cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga
     1381 cccaagctgg ctaggtaagc ttggtaccgc caccatggac tacaaggacg acgatgacaa
     1441 gctcgatgga ggatacccct acgacgtgcc cgactacgcc ggaggactcg actctcgatc
     1501 ccgccatgca aggccttcca gattagtcag gaaggaagat gtaaacaaaa aaaagaaaaa
     1561 cagccaacta cgaaagacaa ccaagggagc caacaaaaat gtggcatcag tcaagacttt
     1621 aagccctgga aaattaaagc aattaattca agaaagagat gttaagaaaa aaacagaacc
     1681 taaaccaccc gtgccagtca gaagccttct gacaagagct ggagcagcac gcatgaattt
     1741 ggataggact gaggttcttt ttcagaaccc agagtcctta acctgcaatg ggtttacaat
     1801 ggcgctacga agcacctctc ttagcaggcg actctcccaa cccccactgg tcgtagccaa
     1861 atccaaaaag gttccacttt ctaagggttt agaaaagcaa catgattgtg attataagat
     1921 actccctgct ttgggagtaa agcactcaga aaatgattcg gttccaatgc aagacaccca
     1981 agtccttcct gatatagaga ctctaattgg tgtacaaaat ccctctttac ttaaaggtaa
     2041 gagccaagag acaactcagt tttggtccca aagagttgag gattccaaga tcaatatccc
     2101 tacccacagt ggccctgcag ctgagatcct tcctgggcca ctggaaggga cacgctgtgg
     2161 tgaaggacta ttctctgaag agacattgaa tgataccagt ggttccccaa aaatgtttgc
     2221 tcaggacaca gtgtgtgctc cttttcccca aagagcaacc cccaaagtta cctctcaagg
     2281 aaaccccagc attcagttag aagagttggg ttcacgagta gaatctctta agttatctga
     2341 ttcttacctg gatcccatta aaagtgaaca tgattgctac cccacctcca gtcttaataa
     2401 ggttatacct gacttgaacc ttagaaactg cttggctctt ggtgggtcta cgtctcctac
     2461 ctctgtaata aaattcctct tggcaggctc aaaacaagcg acccttggtg ctaaaccaga
     2521 tcatcaagag gccttcgaag ctactgcaaa tcaacaggaa gtttctgata ccacctcttt
     2581 cctaggacag gcctttggtg ctatcccaca tcaatgggaa cttcctggtg ctgacccagt
     2641 tcatggtgag gccctgggtg agaccccaga tctaccagag attcctggtg ctattccagt
     2701 ccaaggagag gtctttggta ctattttaga ccaacaagaa actcttggta tgagtgggag
     2761 tgttgtccca gacttgcctg tcttccttcc tgttcctcca aatccaattg ctacctttaa
     2821 tgctccttcc aaatggcctg agccccaaag cactgtctca tatggacttg cagtccaggg
     2881 tgctatacag attttgcctt tgggctcagg acacactcct caatcatcat caaactcaga
     2941 gaaaaattca ttacctccag taatggctat aagcaatgta gaaaatgaga agcaggttca
     3001 tataagcttc ctgccagcta acactcaggg gttcccatta gcccctgaga gaggactctt
     3061 ccatgcttca ctgggtatag cccaactctc tcaggctggt cctagcaaat cagacagagg
     3121 gagctcccag gtcagtgtaa ccagcacagt tcatgttgtc aacaccacag tggtgactat
     3181 gccagtgcca atggtcagta cctcctcttc ttcctatacc actttgctac cgactttgga
     3241 aaagaagaaa agaaagcgat gtggggtctg tgaaccctgc cagcagaaga ccaactgtgg
     3301 tgaatgcact tactgcaaga acagaaagaa cagccatcag atctgtaaga aaagaaaatg
     3361 tgaggagctg aaaaagaaac catctgttgt tgtgcctctg gaggttataa aggaaaacaa
     3421 gaggccccag agggaaaaga agcccaaagt tttaaaggca gattttgaca acaaaccagt
     3481 aaatggcccc aagtcagaat ccatggacta cagtagatgt ggtcatgggg aagaacaaaa
     3541 attggaattg aacccacata ctgttgaaaa tgtaactaaa aatgaagaca gcatgacagg
     3601 catcgaggtg gagaagtgga cacaaaacaa gaaatcacag ttaactgatc acgtgaaagg
     3661 agattttagt gctaatgtcc cagaagctga aaaatcgaaa aactctgaag ttgacaagaa
     3721 acgaaccaaa tctccaaaat tgtttgtaca aaccgtaaga aatggcatta aacatgtaca
     3781 ctgtttacca gctgaaacaa atgtttcatt taaaaaattc aatattgaag aattcggcaa
     3841 gacattggaa aacaattctt ataaattcct aaaagacact gcaaaccata aaaacgctat
     3901 gagctctgtt gctactgata tgagttgtga tcatctcaag gggagaagta acgttttagt
     3961 attccagcag cctggcttta actgcagttc cattccacat tcttcacact ccatcataaa
     4021 tcatcatgct agtatacaca atgaaggtga tcaaccaaaa actcctgaga atataccaag
     4081 taaagaacca aaagatggat ctcccgttca accaagtctc ttatcgttaa tgaaagatag
     4141 gagattaaca ttggagcaag tggtagccat agaggccctg actcaactct cagaagcccc
     4201 atcagagaat tcctccccat caaagtcaga gaaggatgag gaatcagagc agagaacagc
     4261 cagtttgctt aatagctgca aagctatcct ctacactgta agaaaagacc tccaagaccc
     4321 aaacttacag ggagagccac caaaacttaa tcactgtcca tctttggaaa aacaaagttc
     4381 atgcaacacg gtggttttca atgggcaaac tactaccctt tccaactcac atatcaactc
     4441 agctactaac caagcatcca caaagtcaca tgaatattca aaagtcacaa attcattatc
     4501 tctttttata ccaaaatcaa attcatccaa gattgacacc aataaaagta ttgctcaagg
     4561 gataattact cttgacaatt gttccaatga tttgcatcag ttgccaccaa gaaataatga
     4621 agtggagtat tgcaaccagt tactggacag cagcaaaaaa ttggactcag atgatctatc
     4681 atgtcaggat gcaacccata cccaaattga ggaagatgtt gcaacacagt tgacacaact
     4741 tgcttcgata attaagatca attatataaa accagaggac aaaaaagttg aaagtacacc
     4801 aacaagcctt gtcacatgta atgtacagca aaaatacaat caggagaagg gcacaatgca
     4861 acagaaacca ccttcaagtg tacacaataa tcatggttca tcattaacaa aacaaaagaa
     4921 cccaacccag aaaaagacaa aatccacccc atcaagagat cggcggaaaa agaagcccac
     4981 agttgtaagt tatcaagaaa atgatcggca gaagtgggaa aagttgtcct atatgtatgg
     5041 cacaatatgc gacatttgga tagcatcgaa atttcaaaat tttgggcaat tttgtccaca
     5101 tgattttcct actgtatttg ggaaaatttc ttcctcgacc aaaatatgga aaccactggc
     5161 tcaaacgagg tccattatgc aacccaaaac agtatttcca ccactcactc agataaaatt
     5221 acagagatat cctgaatcag cagaggaaaa ggtgaaggtt gaaccattgg attcactcag
     5281 cttatttcat cttaaaacgg aatccaacgg gaaggcattc actgataaag cttataattc
     5341 tcaggtacag ttaacggtga atgccaatca gaaagcccat cctttgaccc agccctcctc
     5401 tccacctaac cagtgtgcta acgtgatggc aggcgatgac caaatacggt ttcagcaggt
     5461 tgttaaggag caactcatgc atcagagact gccaacattg cctggtatct ctcatgaaac
     5521 acccttaccg gagtcagcac taactctcag gaatgtaaat gtagtgtgtt caggtggaat
     5581 tacagtggtt tctaccaaaa gtgaagagga agtctgttca tccagttttg gaacatcaga
     5641 attttccaca gtggacagtg cacagaaaaa ttttaatgat tatgccatga acttctttac
     5701 taaccctaca aaaaacctag tgtctataac taaagattct gaactgccca cctgcagctg
     5761 tcttgatcga gttatacaaa aagacaaagg cccatattat acacaccttg gggcaggacc
     5821 aagtgttgct gctgtcaggg aaatcatgga gaataggtat ggtcaaaaag gaaacgcaat
     5881 aaggatagaa atagtagtgt acaccggtaa agaagggaaa agctctcatg ggtgtccaat
     5941 tgctaagtgg gttttaagaa gaagcagtga tgaagaaaaa gttctttgtt tggtccggca
     6001 gcgtacaggc caccactgtc caactgctgt gatggtggtg ctcatcatgg tgtgggatgg
     6061 catccctctt ccaatggccg accggctata cacagagctc acagagaatc taaagtcata
     6121 caatgggcac cctaccgaca gaagatgcac cctcaatgaa aatcgtacct gtacatgtca
     6181 aggaattgat ccagagactt gtggagcttc attctctttt ggctgttcat ggagtatgta
     6241 ctttaatggc tgtaagtttg gtagaagccc aagccccaga agatttagaa ttgatccaag
     6301 ctctccctta catgaaaaaa accttgaaga taacttacag agtttggcta cacgattagc
     6361 tccaatttat aagcagtatg ctccagtagc ttaccaaaat caggtggaat atgaaaatgt
     6421 tgcccgagaa tgtcggcttg gcagcaagga aggtcgtccc ttctctgggg tcactgcttg
     6481 cctggacttc tgtgctcatc cccacaggga cattcacaac atgaataatg gaagcactgt
     6541 ggtttgtacc ttaactcgag aagataaccg ctctttgggt gttattcctc aagatgagca
     6601 gctccatgtg ctacctcttt ataagctttc agacacagat gagtttggct ccaaggaagg
     6661 aatggaagcc aagatcaaat ctggggccat cgaggtcctg gcaccccgcc gcaaaaaaag
     6721 aacgtgtttc actcagcctg ttccccgttc tggaaagaag agggctgcga tgatgacaga
     6781 ggttcttgca cataagataa gggcagtgga aaagaaacct attccccgaa tcaagcggaa
     6841 gaataactca acaacaacaa acaacagtaa gccttcgtca ctgccaacct tagggagtaa
     6901 cactgagacc gtgcaacctg aagtaaaaag tgaaaccgaa ccccatttta tcttaaaaag
     6961 ttcagacaac actaaaactt attcgctgat gccatccgct cctcacccag tgaaagaggc
     7021 atctccaggc ttctcctggt ccccgaagac tgcttcagcc acaccagctc cactgaagaa
     7081 tgacgcaaca gcctcatgcg ggttttcaga aagaagcagc actccccact gtacgatgcc
     7141 ttcgggaaga ctcagtggtg ccaatgcagc tgctgctgat ggccctggca tttcacagct
     7201 tggcgaagtg gctcctctcc ccaccctgtc tgctcctgtg atggagcccc tcattaattc
     7261 tgagccttcc actggtgtga ctgagccgct aacgcctcat cagccaaacc accagccctc
     7321 cttcctcacc tctcctcaag accttgcctc ttctccaatg gaagaagatg agcagcattc
     7381 tgaagcagat gagcctccat cagacgaacc cctatctgat gaccccctgt cacctgctga
     7441 ggagaaattg ccccacattg atgagtattg gtcagacagt gagcacatct ttttggatgc
     7501 aaatattggt ggggtggcca tcgcacctgc tcacggctcg gttttgattg agtgtgcccg
     7561 gcgagagctg cacgctacca ctcctgttga gcaccccaac cgtaatcatc caacccgcct
     7621 ctcccttgtc ttttaccagc acaaaaacct aaataagccc caacatggtt ttgaactaaa
     7681 caagattaag tttgaggcta aagaagctaa gaataagaaa atgaaggcct cagagcaaaa
     7741 agaccaggca gctaatgaag gtccagaaca gtcctctgaa gtaaatgaat tgaaccaaat
     7801 tccttctcat aaagcattaa cattaaccca tgacaatgtt gtcaccgtgt ccccttatgc
     7861 tctcacacac gttgcggggc cctataacca ttgggtctga tctagagggc ccttcgaagg
     7921 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     7981 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8041 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8101 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8161 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8221 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8281 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8701 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8761 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8881 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8941 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9001 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9061 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     9121 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     9181 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     9241 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     9301 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     9361 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     9421 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     9481 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     9541 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     9601 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     9661 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     9721 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     9781 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9841 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     9901 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
     9961 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10081 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10201 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10261 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    11101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    11341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    11761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    12001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    12121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    12181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    12241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    12301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    12361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    12421 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    12481 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    12541 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggtt
//
