LOCUS       sequence-244818-        2598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P16
FEATURES             Location/Qualifiers
     source          1..2598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(963..997)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1068..1727)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1642..1661
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1909..2497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2398..2417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acggagcgac aaacacactc ccacatacat ctttcccgga ggcagaaaca
       61 aaataagaac aacaacaaca caccaacacg ccaaccatag taacccacac gcttaaacaa
      121 ccagtatcag accggtaccc aacttctacg gccggcggcg gctggcagcg gcctcgagca
      181 tcctctattc cctcacctct tctctcgctg ttgtacaact tacaggaatt cccctctctc
      241 ctcgaactgg aagccgtgtt tcacgtgagt ggtggtacga tacccggtgg gaaccctgtg
      301 ttgtgatatt tcgccttctg gtttttgttt ttgtctctcc cctctggctt ccaataccgg
      361 aaaatttgga aattttggaa aaacaggaaa tcacaaaaaa acaggagaac ctggaaaacg
      421 taacgaaacc aaatagtgga acccaactga atccgccgta gaccagatca acccacatga
      481 ttaaacgtcg ccctcgactt ccagtggtac ttccagtacc aattccagta ccactttgaa
      541 caactctcca taacacttcc aattccactt acagtgtatg tgtgtgtatg tgtgtgcgtg
      601 tgtggacagg tttcttgcgc ttcttgtact tctggtgatg atagatggtg gattctcggt
      661 acaggaattg gctcaggctt tttttttttc acatcgattt cagtttgggc tttccctatg
      721 cattcattat attgtgattg cattgagctt tggaaatttt tcatactctt cgaaatcata
      781 tataataagt tacaatgtga gttattctct ccttcctggt tgttggtgta agcatcattt
      841 aactagtccc ttttttttaa agttatttaa tatatatttt tattttgtcc caaacaccaa
      901 gaagtaatct ttacttttag cacacaaata accaataaat tctaatatgt gagaccagac
      961 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1021 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1081 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1141 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1201 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1261 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
     1321 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1381 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1441 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1501 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1561 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1621 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1681 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1741 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1801 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     1861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2581 ttctgtggat aaccgtag
//
