LOCUS       sequence-244826-        2666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P5
FEATURES             Location/Qualifiers
     source          1..2666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1031..1065)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1136..1795)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1710..1729
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2466..2485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtcattat tattattcat tattccactt ctcgttatta cctcagatat
       61 acaccgtttg tacgtgagaa tgagagctta gccccaatct tagtaaatca agagtaagta
      121 aaaaaaacac taaaaaaaaa taattacagt gccattagta ctagtagtac tagtaattag
      181 tgggtgtatg cggaatagta agtacgagca agagccctac ctactaatgc cgctaccact
      241 attatgctat tccgccttgg gttgccaagg ccctggggcc cggtggattt ctacttccta
      301 cctcactcac acgcccgcag caacgacttc actacccgaa taagaatgtc gatgtcatag
      361 gtaaatacca tagcgacaac ctactgccat tgcaacaagc aaatatcacc ctctaatggt
      421 tgtggctttt gcaagaattc ggatccaaac cgaattgatt tcgatttgat ttgctcatct
      481 aactattcct taagaagtaa agaaatgatt ggaatcagga accgaaggcg ttccccatta
      541 acaacctcgc acaaagatgt aaaaaaattc tttgaatgat cctactttat tttttttttt
      601 tttttgattt gtgcttctgc ttcctcttat tctgcggcct ttttctcgag attttctcat
      661 tttgttccta tttggatccc acttagttct caattcggaa aagctattgg caaagaacag
      721 gtgagagggg aaggttttgg caggaaagtt atttttagaa cggtttatcc acaccatacc
      781 atagtatcta ccattagtat tgtttgctcg atgccccaaa gcaaagaaag gattagagat
      841 gttgaaaggt tttcgaaatt gtatataaag agaggatgga tgagatgtta atttggaatc
      901 tgtttgtctt gtatgtatct atatctgttg tagattatat cttgaataac actcaaaaca
      961 gtaatcaaca gatccaatcg tttttaccaa cacacacata ccccgattta tactatgtga
     1021 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1081 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1141 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1201 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1261 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1321 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1381 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1441 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1501 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1561 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1621 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1681 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1741 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1801 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1861 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtag
//
