LOCUS       sequence-244827-        2660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P9
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1025..1059)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1130..1789)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1704..1723
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1971..2559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2460..2479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtgtacaa attatggtat taggtaatga taattaactg aaacggctct
       61 tttcgcagaa aaatcggagt gcaaaaactt gtttcgcctt cattttccat tgtttttttt
      121 tttttttata tttgtgccat gcattccatt ctgcaggtgg tttccttttt ggaacctttg
      181 gtgtagttac ggtgagcctc tccgactgaa gctagatatc attgactcca cgaatttagt
      241 gtcaaaaaga aatccgtttc cttgggatcc ccacttttac cttgtagcga cttaagattc
      301 acagttatcg gctgtgtacc gaccggtcta tagggatgag gctgtagtac tagcttttct
      361 gctaataatg ttcttccgtg tctcttattt caacgtttgg ctggttattt ctccagttac
      421 ggtaatcgga gaggggaagt ttgagtgcaa gagaggggca gccgtaagat gtgttggttg
      481 cgaaagaagg cactgagggg taattgtaaa atggttatgc gaactatcgg ggtctagtaa
      541 agccaaggtc acacatactg cccgtttgta atcagattga gaatttggac gtaataacgt
      601 agagatacat atctatctac gtcagtcggt ccaaagcaaa cagccgtgtt gttttttatt
      661 ttattcctac ttacctcatc cttgaacttc aatatcaaca tatataaatg aatgtttctc
      721 tctaagtgct tcctcgtttt gatacgttgt gagtctggag cctagtctaa actaaccatt
      781 gtctgcaaaa tcgtcaagca attgctaaaa acccccattg cgtcattctt tttgagtaga
      841 gtgtgttaag tttattcgca agatttattc gcaactggtc attttagaca atacatcttc
      901 atttggttta ttcagcttta aggggaacgg tcttaatagt tatcggatct ttacctaaaa
      961 aaaaaaaacg gattttgtaa attcctagaa tcacacgtgc acaaaactat gtgagaccag
     1021 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1081 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1141 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1201 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1261 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1321 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1381 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1441 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1501 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1561 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1621 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1681 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1741 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1801 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1861 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     1921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2641 gattctgtgg ataaccgtag
//
