LOCUS       sequence-244828-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus centromere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.C3
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1205..1239)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1310..1969)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1884..1903
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2151..2739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2640..2659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agttgatcca agtctgaagg ttggtttggc actaacttta ctcttgttat
       61 attcagaatt gtatcaagtt tatttgttag agtggagcct ttttttatcc gtaacacttt
      121 ttccctgctc cattttgaaa aacgatttaa ggccatcttg gctattccga atgaatttgg
      181 aatatgttta aattaataaa aaaaaataaa ataaaataaa ataaaataaa aattaaatca
      241 aattaaatta aattaaatta aattaaatta aataaaaata aatacaacca atacaaccaa
      301 tacaacatgg tagtattctt gcgtcgtaat gaatattaaa tatcacttta ttaatctcat
      361 cgtgttttat tgtttttgta aggactttaa tatatttgaa tcaatattct ttcaattact
      421 agtacttttt ttatatgact aaaattgtta cacattggac tgacagttat ttttaaaatt
      481 tatgatttat tcttacttta tatctttaaa agtagaaata ttatacggac gctttgaatg
      541 aaattgacaa cttatcttac tagtgtgaat caaccctatc gatgtagtac tctcaaaata
      601 cggccttctt gataaagtgt taaattcatt tgggtaatga tttttcgaaa accacattga
      661 atgaacgatt taaataaata taggatgcaa gagcatttta ataattcaga aacaaacaaa
      721 ttattaaaca ggagcagttg aacggtatgt tagcgagttt tgtaaagggt gagtacattt
      781 atagctctat tgaacataat aaatacatat aaatagtatt ttttgaccct ctatgaagat
      841 ggcttaccag caacttatgt gttttaattc acgtgactac taaacaaaaa aatatgttat
      901 ttaaaaaata tttatttaaa tttttaaact attatagatt atttgtgaat acattatttt
      961 ttaatttatt aattaaaaga attgctattt aattaaaata agaataaaag ctttytattt
     1021 ttttaaaaga aaaatatatt aaaaacactt ttccgaaagt taaaataatt ttatacttat
     1081 cggtagctgc aatttataga cataatattt tatatttttt taaaatttat tattattttg
     1141 tttgaaataa taacgttggt gagtgtttaa ggtgaactaa gactgaaccg atgagaccag
     1201 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1261 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1321 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1381 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1441 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1501 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1561 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1621 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1681 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1741 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1801 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1861 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1921 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1981 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2041 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     2101 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2161 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2221 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2281 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2341 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2401 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2461 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2521 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2581 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2641 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2701 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2761 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2821 gattctgtgg ataaccgtag
//
