LOCUS       sequence-244829-        1866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus terminator; type 4 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.T5
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(231..265)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(336..995)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     910..929
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1177..1765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1666..1685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgtgcatc attgaccttt ctaatgaaac cacacgtgaa ttaaaggaaa
       61 gcgcgcagca gcagcagcag cttttctttc cttcttcaaa tcataccttt tctctatttc
      121 aacgttcttt tcatttcacc atgaaatgag ttgaattgtt gctttgagtt tacgacgata
      181 cataaatatt aattaaaata cgaaaaatac acagctgtga gaccagacca ataaaaaacg
      241 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      301 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      361 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      421 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      481 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      541 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      601 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      661 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      721 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      781 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      841 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      901 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      961 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1021 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1081 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1321 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1741 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1801 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1861 ccgtag
//
